@article{ZAINOL HILMI_IDRIS_MAIZATUL-SURIZA_MADIHAH_NUR-RASHYEDA_2022, title={MOLECULAR PCR ASSAYS FOR DETECTION OF Ganoderma PATHOGENIC TO OIL PALM IN MALAYSIA }, volume={51}, url={https://jms.mabjournal.com/index.php/mab/article/view/2201}, DOI={10.55230/mabjournal.v51i1.2201}, abstractNote={<p><em>Ganoderma boninense</em> is a fungal pathogen that causes basal stem rot (BSR) disease in oil palm. Being a serious disease problem to the oil palm industry, monitoring and detecting the pathogen is of the utmost importance to reduce disease spread and facilitate effective management strategies. Because traditional culture-based assay is time-consuming, labour-intensive and required special skills in mycology, plant pathologists are turning to more accurate, sensitive and fast methods such as molecular techniques. In this study, polymerase chain reaction (PCR) assays were developed to detect pathogenic <em>Ganoderma</em> species causing BSR disease in oil palm using a primer designed based on the ribosomal DNA internal transcribed spacer (ITS) region. The effectiveness of conventional and real-time PCR assays was analysed compared to the traditional isolation-based assay. For artificially inoculated oil palm plantlets, consistent detection of <em>G. boninense</em> was observed. Real-time PCR assay has shown to be more sensitive and rapid in detecting <em>G. boninense</em> in field samples and could potentially serve as a validation tool to other detection techniques for implementation of effective disease control measures.</p>}, number={1}, journal={Malaysian Applied Biology}, author={ZAINOL HILMI, NUR HAILINI and IDRIS, ABU SEMAN and MAIZATUL-SURIZA, MOHAMED and MADIHAH, AHMAD ZAIRUN and NUR-RASHYEDA, RAMLI}, year={2022}, month={Mar.}, pages={171–182} }