@article{MULNIA HANIF_HON TJONG_SYAIFULLAH_SANTOSO_EFRIZAL_IMELDA ROESMA_2022, title={EXPRESSION OF BCL-2 AND BAX GENES INDUCED BY AN OVARIAN EXTRACT OF SINGKARAK LAKE PUFFERFISH (Tetraodon leiurus) IN BREAST CANCER CELL}, volume={51}, url={https://jms.mabjournal.com/index.php/mab/article/view/2283}, DOI={10.55230/mabjournal.v51i3.2283}, abstractNote={<p class="p1">It is known that the ovarian extract of Singkarak Lake Pufferfish <em>(Tetraodon leiurus)</em> has the potential for chemoprevention in MCF-7 cells. However, the expression of <em>Bcl-2</em> and <em>Bax</em> genes was associated with cancer. Therefore, this study aimed to analyze the <em>Bcl-2</em> and <em>Bax</em> gene expression induced by the ovarian extract of Singkarak Lake Pufferfish <em>(Tetraodon leiurus)</em> in MCF-7. The method in this study was an in vitro experiment using MCF-7 control cells and MCF-7 cells induced by the ovarian extract of Singkarak Lake Pufferfish <em>(Tetraodon leiurus)</em>, and Real-time PCR was used to examine the expression of <em>Bcl-2</em> and <em>Bax</em> genes. The result showed that the expression of the <em>Bcl-2</em> gene had an insignificant decrease (<em>p</em>>0.05) by 15%, and the expression of the <em>Bax</em> gene had a significant increase (<em>p</em><0.05) by 489% compared to the control. These results support that ovarian extract Singkarak Lake Pufferfish <em>(Tetraodon leiurus)</em> can be alternative cancer chemoprevention. <span class="Apple-converted-space"> </span></p>}, number={3}, journal={Malaysian Applied Biology}, author={MULNIA HANIF, MONICA and HON TJONG, DJONG and SYAIFULLAH and SANTOSO, PUTRA and EFRIZAL and IMELDA ROESMA, DEWI}, year={2022}, month={Sep.}, pages={63–69} }