@article{MOHD AMIN_RUSLAN_ZULKIPLI_ZAKARIA_MAT JALIL_ARIS_2022, title={CYTOTOXIC EFFECT OF DILLAPIOLE ON HUMAN BREAST CANCER MCF-7 CELLS}, volume={51}, url={https://jms.mabjournal.com/index.php/mab/article/view/2502}, DOI={10.55230/mabjournal.v51i4.08}, abstractNote={<p><span style="font-weight: 400;">Plant secondary metabolites and their derivatives play a significant role in anticancer drug therapy since they are effective against specific characteristics while reducing side effects. Dillapiole is a phenylpropanoid that holds several bioactivities like anti-fungal, anti-inflammatory, anti-leishmanial, and anti-cancer. This study aims to investigate the possible cytotoxic effect of dillapiole on human breast cancer, MCF-7 cells. Cells were cultured in complete RPMI media and incubated at standard culture conditions. After the cells reached 80% confluency, cells were treated with various concentrations (ranging from 0 µM to 150 µM) of dillapiole and tamoxifen as a positive control. Cells were later incubated at 48 and 72 hours. Using WST-1 assay, the cytotoxic effect was determined for both incubation times. Results show tamoxifen inhibited the MCF-7 cells with the IC</span><span style="font-weight: 400;">50</span><span style="font-weight: 400;"> at 75.9 µM and 39.8 µM for 48 and 72 hours respectively. Parallel with the positive control results, there was a significant cytotoxic effect of dillapiole against MCF-7 cells at 48- and 72-hours incubation with the IC</span><span style="font-weight: 400;">50 </span><span style="font-weight: 400;">at 92.1 µM and 63.1 µM respectively. Based on these results, dillapiole was cytotoxic against MCF-7 cells and its cytotoxic activity was both in a time and dose-dependent manner (<0.05). The morphological analysis supported the WST-1 assay. Our preliminary finding is in agreement with other previous studies, suggesting that dillapiole appears to be a promising anti-cancer agent and opens a wider possibility of downstream analysis on its underlying cytotoxicity mechanism.</span></p>}, number={4}, journal={Malaysian Applied Biology}, author={MOHD AMIN, INDAH and RUSLAN, NUR BATRISYIA and ZULKIPLI, ZULINDA AYU and ZAKARIA, NURUL AILI and MAT JALIL, MOHD TAUFIQ and ARIS, FARIZAN}, year={2022}, month={Oct.}, pages={29–35} }