@article{MD ZAHIR_IBERAHIM_IBRAHIM_SYED MOHAMAD_RUSMIN_JAILANI_ZULKIFLI_2022, title={ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF CUTTLEBONE CHITOSAN AGAINST Escherichia coli, Staphylococcus aureus AND Candida albicans}, volume={51}, url={https://jms.mabjournal.com/index.php/mab/article/view/2512}, DOI={10.55230/mabjournal.v51i4.17}, abstractNote={<p><span class="fontstyle0">The discovery of new antibiotics for infectious diseases has become challenging due to the rise of antimicrobial resistance.<br />Meanwhile, chitosan has been considerably used in many branches of research. It has been discovered to have some good benefits<br />in medicals, pharmaceuticals, and food technologies. In this study, chitosan was prepared from the cuttlebone of </span><span class="fontstyle2"><em>Sepia</em> </span><span class="fontstyle0">sp. by<br />chemical method and analyzed by using FT-IR spectrophotometer for the confirmed presence of its functional groups. There<br />are three types of reactive functional groups in the chitosan which are the amino group and primary and secondary hydroxyl<br />group attached to the C-2, C-3, and C-6 positions respectively. Chitosan has a high cationic property due to the presence of its<br />amino group. The bacteriostatic activity of chitosan occurs due to its positive charge in acidic concentration that interacts with<br />the negatively charged residue of carbohydrates, lipids, and proteins located on the cell surface of bacteria. The antioxidant<br />activity was conducted using DPPH radical scavenging assay with a chitosan concentration ranging from 0.1 to 10 mg/mL and<br />a hydrogen peroxide scavenging assay with a chitosan concentration ranging from 0.1 to 1.6 mg/mL. The antimicrobial activity<br />of chitosan from cuttlebone was analyzed against two different bacterial strains (</span><em><span class="fontstyle2">Escherichia coli </span></em><span class="fontstyle0">& </span><em><span class="fontstyle2">Staphylococcus aureus</span></em><span class="fontstyle0">)<br />and a fungal strain, </span><em><span class="fontstyle2">Candida albicans </span></em><span class="fontstyle0">by disc diffusion and minimum inhibitory concentration (MIC) method. The results show<br />that through the DPPH radical assay, the scavenging activity was 59.7% at the concentration of chitosan at 10mg/mL, while<br />through the hydrogen peroxide assay the scavenging activity was 56% at the concentration of chitosan at 1.6 mg/mL. Besides,<br />this chitosan from </span><em><span class="fontstyle2">Sepia </span></em><span class="fontstyle0">sp. has concentration-dependent antimicrobial activity with higher antifungal activity compared to<br />antibacterial activity against all tested organisms and may become a potential agent for antibiotic discovery</span> </p>}, number={4}, journal={Malaysian Applied Biology}, author={MD ZAHIR, MASNITA FATIHAH and IBERAHIM, RASHIDAH and IBRAHIM, NAZLINA and SYED MOHAMAD, SHARIFAH AMINAH and RUSMIN, RUHAIDA and JAILANI, NOOR ATIKAH and ZULKIFLI, NURUL ILLIYA ZAFIRAH}, year={2022}, month={Oct.}, pages={103–108} }