TY - JOUR AU - ROSLI, NUR SYUHADA AU - JOHN BAPTIST, ELVIE AU - HARUN, NOR OMAIMA PY - 2019/03/20 Y2 - 2024/03/29 TI - BACTERIA ISOLATION FROM SKIN, GILLS AND WATER OF Oreochromis sp. (RED TILAPIA) WHEN TREATED WITH Eisenia foetida (OLIGOCHAETE), Pleurotus sajor-caju (OYSTER MUSHROOM), AND Nepenthes gracilis (PITCHER PLANT) JF - Malaysian Applied Biology JA - MAB VL - 48 IS - 1 SE - Research Articles DO - UR - https://jms.mabjournal.com/index.php/mab/article/view/2292 SP - 67-72 AB - <p><span class="fontstyle0">Aquaculture farmers are focused on large intensive farming to fulfill the demand of protein source, thus increasing the probability of bacteria-related disease outbreak. Therefore, an alternative fish diet has to be introduced to reduce bacteria related infection while increasing the production. The aim of this study was to determine the microbial community on </span><em><span class="fontstyle2">Oreochromis </span></em><span class="fontstyle0">sp. when treated with modified diets; commercial pellet added with oligochaete (</span><em><span class="fontstyle2">Eisenia foetida</span></em><span class="fontstyle0">), oyster mushroom (</span><em><span class="fontstyle2">Peratus sajor</span></em><span class="fontstyle2">-</span><em><span class="fontstyle2">caju</span></em><span class="fontstyle0">), pitcher plant (</span><em><span class="fontstyle2">Nepenthes gracilis</span></em><span class="fontstyle0">) and combination of all ingredients with proportion of (90:10) and (70:10:10:10). One hundred ninety bacterial colonies from 11 species were identified using biochemical tests: Gram stain, Oxidase test, Catalase test, KOH String test, Carbohydrate (Glucose, Lactose and Sucrose) test, Indole test, Methyl Red Test and Voges-Proskauer Test (MRVP test), Citrate test and bacteria growth tested on Mannitol salt agar. </span><span class="fontstyle2">Oreochromis </span><span class="fontstyle0">sp. also secretes mucus that consists of protein to protect fish from pathogens with various environment conditions. Therefore, this study can be used to increase a better understanding of modified organic diet with higher protein content of microbial community in lower vertebrates.</span></p> ER -