TY - JOUR AU - ISMAIL, NABILAH PY - 2019/03/20 Y2 - 2024/03/28 TI - A PRELIMINARY STUDY OF PHYTOCHEMICAL CONTENTS IN COMMERCIAL MALAYSIAN BLACK TEA EXTRACT USING QUANTITATIVE AND QUALITATIVE TESTS JF - Malaysian Applied Biology JA - MAB VL - 48 IS - 1 SE - Research Articles DO - UR - https://jms.mabjournal.com/index.php/mab/article/view/2302 SP - 123-129 AB - <p><span class="fontstyle0">Black tea (</span><em><span class="fontstyle2">Camellia sinensis</span></em><span class="fontstyle0">) is a flavoured, functional, and therapeutic non-alcoholic drink that is consumed by two-thirds of the world’s population. In this study, the bioactive compounds in black tea leaves were extracted using two different solvents which are methanol and acetone before being further characterized with FTIR to identify the functional groups. The presence of active compounds such as gallic tannin, catecholin tannin, phenol and flavonoid compounds were further tested with qualitative and quantitative methods. Based on FTIR results, the best solvent to extract tea leaves was acetone. The frequency obtained for phenolic –OH stretch was 3390.5 cm</span><sup><span class="fontstyle0">-1</span></sup><span class="fontstyle0">, while alkane –CH</span><sub><span class="fontstyle0">3 </span></sub><span class="fontstyle0">stretch appeared at 2921.4 cm</span><sup><span class="fontstyle0">-1 </span></sup><span class="fontstyle0">and 2850.7 cm</span><sup><span class="fontstyle0">-1</span></sup><span class="fontstyle0">. The UV-Vis spectrum was obtained and the </span><span class="fontstyle3">λ </span><span class="fontstyle0">max from the spectrum is 270 nm. Qualitative analysis involving ferric chloride and lead acetate test showed positive results for the presence of gallic tannin, catecholin, flavonoid and phenols in the tea leaves extract. From the quantitative test, the total phenolic content was 0.9217 mg gallic acid equivalent (GAE)/L while the concentration of flavonoid content was 0.7792 mg quercetin/L and total tannin content 0.13 g/g respectively.</span></p> ER -