EXPRESSION PATTERNS OF THE HUMAN RIBOSOMAL PROTEIN GENES eL14 AND uS19 IN COLON CANCER IS DEPENDENT ON THE TYPE AND STAGE OF THE CANCER CELL

https://doi.org/10.55230/mabjournal.v49i1.1652

Authors

  • EDMUND UI-HANG SIM Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • SELVAMALAR MUTSAMY Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
  • ZY-YING TEH Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia

Keywords:

Ribosomal protein genes, colon cancer, transcript level, eL14, uS19

Abstract

Although the association of some ribosomal protein genes with colorectal cancer is widely known, the detailed mechanisms and complete list of associated genes is lacking. More importantly, the behaviours of these genes in different types and stages of the cancer are poorly understood. Herein we report the study of two ribosomal protein genes in cell lines derived from different sites and stages of colon cancer. Specifically, we analysed the expression pattern of eL14 and uS19 in HCT116 and SW480 cell lines. These two genes, although associated with a wide variety of cancer types, are poorly or have not been studied in colorectal cancer. Semi-quantitative reverse transcription – polymerase chain reaction (RT-PCR) approach was used, together with Students’ t-test validation. We found a significantly (p < 0.05) differential eL14 and uS19 expression patterns between HCT116 and SW480 cell lines. Our findings suggest that eL14 and uS19 have higher activity in a poorly differentiated cell line derived from advanced (metastatic) stage (Duke’s Stage D) colorectal carcinoma tissues compared to the moderately differentiated cell line derived from a mid-stage (Duke’s Stage B) colorectal adenocarcinoma tumour. This will have important implications for both ribosomal protein genes as type and stage specific biomarkers for colon cancer

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References

Ban, N., Beckmann, R., Cate, J.H., Dinman, J.D., Dragon, F., Ellis, S.R., Lafontaine, D.L., Lindahl, L., Liljas, A., Lipton, J.M. & McAlear, M.A. 2014. A new system for naming ribosomal proteins. Current Opinions in Structural Biology, 24: 165-169. https://doi.org/10.1016/j.sbi.2014.01.002 DOI: https://doi.org/10.1016/j.sbi.2014.01.002

Bretones, G., Álvarez, M.G., Arango, J.R., Rodríguez, D., Nadeu, F., Prado, M.A., ValdesMas, R., Puente, D.A., Paulo, J.A., Delgado, J., Villamor, N., Lopez-Guillermo, A., Finley, D.J., Gygi, S.P., Campo, E., Quesada, V. & LopezOtin, C. 2018. Altered patterns of global protein synthesis and translational fidelity in RPS15- mutated chronic lymphocytic leukemia. Blood, The Journal of the American Society of Hematology, 132(22): 2375-2388. https://doi.org/10.1182/blood-2017-09-804401 DOI: https://doi.org/10.1182/blood-2017-09-804401

Chomczynski, P. & Sacchi, N. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry, 162(1): 156-159. https://doi.org/10.1016/0003-2697(87)90021-2 DOI: https://doi.org/10.1016/0003-2697(87)90021-2

Daftuar, L., Zhu, Y., Jacq, X. & Prives, C. 2013. Ribosomal proteins RPL37, RPS15 and RPS20 regulate the Mdm2-p53-MdmX network. PLoS One, 8(7): e68667. https://doi.org/10.1371/journal.pone.0068667 DOI: https://doi.org/10.1371/journal.pone.0068667

Fang, W., Li, X., Jiang, Q., Liu, Z., Yang, H., Wang, S., Xie, S., Liu, Q., Liu, T., Huang, J. & Xie, W. 2008. Transcriptional patterns, biomarkers and pathways characterizing nasopharyngeal carcinoma of Southern China. Journal of Translational Medicine, 6(32): 1-13. https://doi.org/10.1186/1479-5876-6-32 DOI: https://doi.org/10.1186/1479-5876-6-32

Flatmark, K., Maelandsmo, G.M., Martinsen, M., Rasmussen, H. & Fodstad, Ø. 2004. Twelve colorectal cancer cell lines exhibit highly variable growth and metastatic capacities in an orthotopic model in nude mice. European Journal of Cancer, 40(10): 1593-1598. https://doi.org/10.1016/j.ejca.2004.02.023 DOI: https://doi.org/10.1016/j.ejca.2004.02.023

Gou, Y., Shi, Y., Zhang, Y., Nie, Y., Wang, J., Song, J., Jin, H., He, L., Gao, L., Qiao, L. & Wu, K. 2010. Ribosomal protein L6 promotes growth and cell cycle progression through upregulating cyclin E in gastric cancer cells. Biochemical and Biophysical Research Communications, 393: 788-793. https://doi.org/10.1016/j.bbrc.2010.02.083 DOI: https://doi.org/10.1016/j.bbrc.2010.02.083

Huang, X.P., Zhao, C.X., Li, Q.J., Cai, Y., Liu, F.X., Hu, H., Xu, X., Han, Y.L., Wu, M., Zhan, Q.M. & Wang, M.R. 2006. Alteration of RPL14 in squamous cell carcinomas and preneoplastic lesions of the esophagus. Gene, 366(1): 161-168. https://doi.org/10.1016/j.gene.2005.09.025 DOI: https://doi.org/10.1016/j.gene.2005.09.025

Kawada, M., Mizuno, M., Nasu, J., Uesu, T., Okazaki, H., Okada, H., Shimomura, H., Yamamoto, K., Tsuji, T., Fujita, T. & Shiratori, Y. 2003. Release of decay-accelerating factor into stools of patients with colorectal cancer by means of cleavage at the site of glycosylphosphatidylinositol anchor. Journal of Laboratory and Clinical Medicine, 142(5): 306-312. https://doi.org/10.1016/S0022-2143(03)00137-9 DOI: https://doi.org/10.1016/S0022-2143(03)00137-9

Kondoh, N., Schweinfest, C.W., Henderson, K.W. & Papas, T.S. 1992. Differential expression of S19 ribosomal protein, laminutesin-binding protein, and human lymphocyte antigen class I messenger RNAs associated with colon carcinoma progression and differentiation. Cancer Research, 52(4): 791-796.

Kreunin, P., Yoo, C., Urquidi, V., Lubman, D.M. & Goodison, S. 2007. Differential expression of ribosomal proteins in a human model identified by coupling 2-D liquid chromatography and mass spectrometry. Cancer Genomics Proteomics, 4(5): 329-339.

Lai, M. & Xu, J. 2007. Ribosomal proteins and colorectal cancer. Genomics, 8: 43-49. https://doi.org/10.2174/138920207780076938 DOI: https://doi.org/10.2174/138920207780076938

Liu, X.Q., Chen, H.K., Zhang, X.S., Pan, Z.G., Li, A., Feng, Q.S., Long, Q.X., Wang, X.Z. & Zeng, Y.X. 2003. Alterations of BLU, a candidate tumour suppressor gene on chromosome 3p21.3, in human nasopharyngeal carcinoma. International Journal of Cancer, 106(1): 60-65. https://doi.org/10.1002/ijc.11166 DOI: https://doi.org/10.1002/ijc.11166

Ljungström, V. & Rosenquist, R. 2018. Not so lost in translation: RPS15 mutations in CLL. Blood, The Journal of the American Society of Hematology, 132(22): 2317-2319. https://doi.org/10.1182/blood-2018-09-875179 DOI: https://doi.org/10.1182/blood-2018-09-875179

Ljungström, V., Cortese, D., Young, E., Pandzic, T., Mansouri, L., Plevova, K., Ntoufa, S., Baliakas, P., Clifford, R., Sutton, L.A. Blakemore, S.J., Stavroyianni, N., Agathangelidis, A., Rossi, D., Höglund, M., Kotaskova, J. Juliusson, G., Belessi, C., Chiorazzi, N., Panagiotidis, P., Langerak, A.W., Smedby, K.E., Oscier, D., Gaidano, G., Schuh, A., Davi, F., Pott, C., Strefford, J.C., Trentin, L., Pospisilova, S., Ghia, P., Stamatopoulos, K., Sjöblom, T. & Rosenquist, R. 2016. Whole-exome sequencing in relapsing chronic lymphocytic leukemia: clinical impact of recurrent RPS15 mutations. Blood, The Journal of the American Society of Hematology, 127(8): 1007-1016. https://doi.org/10.1182/blood-2015-10-674572 DOI: https://doi.org/10.1182/blood-2015-10-674572

Mao, Q., Zhang, P.H., Yang, J., Xu, J.D., Kong, M., Shen, H., Zhu, H., Bai, M., Zhou, L., Li, G., Wang, Q. & Li, S. 2016. iTRAQ-based proteomic analysis of Ginsenoside F2 on human gastric carcinoma cells SGC7901. Evidence-Based Complementary and Alternative Medicine, 2016: Article ID 2635483. https://doi.org/10.1155/2016/2635483 DOI: https://doi.org/10.1155/2016/2635483

Niu, N., Zhang, J., Huang, T., Sun, Y., Chen, Z., Yi, W., Korteweg, C., Wang, J. & Gu, J. 2012. IgG expression in human colorectal cancer and its relationship to cancer behaviors. PLoS One, 7(11): 247362. https://doi.org/10.1371/journal.pone.0047362 DOI: https://doi.org/10.1371/journal.pone.0047362

Pogue-Geile, K., Geiser, J.R., Shu, M., Miller, C., Wool, I.G., Meisler, A.I. & Pipas, J.M. 1991. Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein. Molecular and Cellular Biology, 11(8): 3842- 3849. https://doi.org/10.1128/mcb.11.8.3842-3849.1991 DOI: https://doi.org/10.1128/mcb.11.8.3842-3849.1991

Rajput, A., San Martin, I.D., Rose, R., Beko, A., LeVea, C., Sharratt, E., Mazurchuk, R., Hoffman, R.M., Brattain, M.G. & Wang, J. 2008. Characterization of HCT116 human colon cancer cells in an orthotopic model. Journal of Surgical Research, 147: 276-281. https://doi.org/10.1016/j.jss.2007.04.021 DOI: https://doi.org/10.1016/j.jss.2007.04.021

Romano, P., Manniello, A., Aresu, O., Armento, M., Cesaro, B. & Parodi, B. 2009. Cell lines database: Structure and recent improvements towards molecular authentication of human cell lines. Nucleic Acid Research, 37: D925-D932. https://doi.org/10.1093/nar/gkn730 DOI: https://doi.org/10.1093/nar/gkn730

Shenoy, N., Kessel, R., Bhagat, T.D., Bhattacharyya, S., Yu, Y., Mcmahon, C. & Verma, A. 2012. Alterations in the ribosomal machinery in cancer and hematologic disorders. Journal of Hematological Oncology, 5(1): 32. https://doi.org/10.1186/1756-8722-5-32 DOI: https://doi.org/10.1186/1756-8722-5-32

Shriver, S.P., Shriver, M.D., Tirpak, D.L., Bloch, L.M., Hunt, J.D., Ferrell, R.E. & Siegfried, J.M. 1998. Trinucleotide repeat length variation in the human ribosomal protein L14 gene (RPL14): localization to 3p21. 3 and loss of heterozygosity in lung and oral cancers. Mutation Research/Mutation Research Genomics, 406(1): 9-23. https://doi.org/10.1016/S1383-5726(98)00006-5 DOI: https://doi.org/10.1016/S1383-5726(98)00006-5

Sim, E.U.H., Chee, C.S.M., Vasudevan, L., Ng, K.L. & Chan, S.L.L. 2018. Selective differential expression of the ribosomal protein genes eL14 and uS19 in a well-differentiated epithelial cell line of nasopharyngeal carcinoma. Malaysian Applied Biology, 47(1): 247-253.

Sulima, S.O., Hofman, I.J., De Keersmaecker, K. & Dinman, J.D. 2017. How ribosomes translate cancer. Cancer Discovery, 7(10): 1069-1087. https://doi.org/10.1158/2159-8290.CD-17-0550 DOI: https://doi.org/10.1158/2159-8290.CD-17-0550

Walter, R.B., Obermoeller, R.D., Moore, D.D., Lacson, J.M., Della Coletta, L., McEntire, B.B., Morizot, D.C. & Nairn, R.S. 1996. Characteristics and mapping of the Xiphophorus maculatus (Teleostei: Poeciliidae) RPS15 gene. Cytogenetics and Cell Genetics, 75: 140-144. https://doi.org/10.1159/000134465 DOI: https://doi.org/10.1159/000134465

Wang, Y., Cheong, D., Chan, S. & Hooi, S.C. 2000. Ribosomal protein L7a gene is up-regulated but not fused to the tyrosine kinase receptor as chimeric trk oncogene in human colorectal carcinoma. International Journal of Oncology, 16(4): 757-819. https://doi.org/10.3892/ijo.16.4.757 DOI: https://doi.org/10.3892/ijo.16.4.757

Yeung, T.M., Gandhi, S.C., Wilding, J.L., Muschel, R. & Bodmer, W.F. 2010. Cancer stem cells from colorectal cancer-derived cell lines. Proceedings of the National Academy of Sciences, 107(8): 3722-3727. https://doi.org/10.1073/pnas.0915135107 DOI: https://doi.org/10.1073/pnas.0915135107

Yoon, S.Y., Kim, J.M., Oh, J.H., Jeon, Y.J., Lee, D.S., Kim, J.H., Choi, J.Y., Ahn, B.M., Kim, S., Yoo, H.S. & Kim, Y.S. 2006. Gene expression profiling of human HBV- and/or HCV associated hepatocellular carcinoma cells using sequence tags. International Journal of Oncology, 29: 315-327. https://doi.org/10.3892/ijo.29.2.315 DOI: https://doi.org/10.3892/ijo.29.2.315

Yu, C., Hong, H., Zhang, S., Zong, Y., Ma, J., Lu, A., Sun, J. & Zheng, M. 2019. Identification of key genes and pathways involved in microsatellite instability in colorectal cancer. Molecular Medicine Reports, 19(3): 2065-2076. https://doi.org/10.3892/mmr.2019.9849 DOI: https://doi.org/10.3892/mmr.2019.9849

Published

30-06-2020

How to Cite

SIM, E. U.-H. ., MUTSAMY , S. ., & TEH, Z.-Y. . (2020). EXPRESSION PATTERNS OF THE HUMAN RIBOSOMAL PROTEIN GENES eL14 AND uS19 IN COLON CANCER IS DEPENDENT ON THE TYPE AND STAGE OF THE CANCER CELL. Malaysian Applied Biology, 49(1), 31–39. https://doi.org/10.55230/mabjournal.v49i1.1652

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Research Articles