GENETIC DIVERSITY OF GREEN CHIRETA (Andrographis paniculata (Burm.f.) Wall. Ex Nees.) FROM INDONESIA BASED ON ISSR AND RAPD MARKERS

MUHAMMAD FAUZI  ARIF; DYAH  SUBOSITI; ANISKA NOVITA  SARI; GANIES RIZA  ARISTYA; INDRA  LESMANA; RINA  SRI KASIAMDARI

doi:10.55230/mabjournal.v49i1.1655

Authors

MUHAMMAD FAUZI ARIF Department of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan Street, Sekip Utara, Bulaksumur, Yogyakarta 55281, Indonesia
DYAH SUBOSITI Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional, Lawu Street No. 11, Tawangmangu, Karanganyar, Central Java 57792, Indonesia
ANISKA NOVITA SARI Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional, Lawu Street No. 11, Tawangmangu, Karanganyar, Central Java 57792, Indonesia
GANIES RIZA ARISTYA Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan Street, Sekip Utara, Bulaksumur, Yogyakarta 55281, Indonesia
INDRA LESMANA Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan Street, Sekip Utara, Bulaksumur, Yogyakarta 55281, Indonesia
RINA SRI KASIAMDARI
rkasiamdari@ugm.ac.id
Laboratory of Plant Systematics, Faculty of Biology, Universitas Gadjah Mada, Teknika Selatan Street, Sekip Utara, Bulaksumur, Yogyakarta 55281, Indonesia

Keywords:

Andrographis paniculata, genetic diversity, ISSR, medicinal plant, RAPD

Abstract

Green chireta or Andrographis paniculata (Burm.f.) Wall. Ex Nees. is a medicinal plant widely used by traditional communities in Indonesia. The purpose of this study was to analyze the genetic diversity of A. paniculata in five mainlands from Indonesia using ISSR and RAPD markers. A total of 50 accessions of A. paniculata were used, DNA was amplified using six ISSR and six RAPD primers, generating 61 loci and 43 loci respectively. Genotype data scoring resulted in the percentage of polymorphism, Polymorphic Information Content, Shannon’s Information Index and unbiased expected heterozygosity. The genetic variation between the locations was measured with Principal Coordinate Analysis (PCoA) and pairwise Nei’s unbiased genetic distance. The data from this study revealed that the variation and diversity from accessions within the same island were low. The genetic variation was higher between accessions from different islands. Analysis of molecular variance (AMOVA) was used to calculate the distribution of the variation between and within the A. paniculata population. The results showed the variation derived from the same location was 34% and from a different location was 66%. Genetic diversity provided information to help the further development and cultivation of A. paniculata in Indonesia