COMPARISON OF GENETIC DIVERSITY OF LEPTIN GENE BETWEEN WILD GOAT AND DOMESTIC GOAT BREEDS IN IRAN

Authors

  • NARGES ESMAEILI ALIABAD VAZIR Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran
  • ALI ESMAEILIZADEH KOSHKOOIEH Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran
  • AHMAD AYTOLAHI MEHRJERDI Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran
  • MOHAMMADREZA MOHAMMADABADI Department of Animal Science, Shahid Bahonar University of Kerman, Kerman, Iran
  • OLENA IVANIVNA BABENKO Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
  • MARYNA VITALIIVNA BUSHTRUK Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
  • SERHII VASYLIOVYCH TKACHENKO Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
  • RUSLANA VOLODYMYRIVNA STAVETSKA Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine
  • NATALIIA IHORIVNA KLOPENKO Department of Animal Science, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine

Keywords:

Diversity, domestic goat, leptin gene, PCR-RFLP, wild goat

Abstract

Leptin is a polypeptide which is mostly secreted by the white adipose tissue and a little by gastrointestinal tract and placenta, plays an important role for controlling body weight, feed intake, immunity, milk production, and reproduction. The aim of this study was to investigate the exon 2 of the leptin gene polymorphism in Iranian wild and domestic goats using PCR- RFLP. Blood samples were collected from 14 wild and domestic goat breeds including (Cashmere Abadeh, Torki-Ghahghaei, Naeini, Robati, Nadoushani, Adani, Shahrbabaki, Birjandi, China goat, Sannen, Pakistani, Raeini cashmere, Najdi, and Wild goat) and then genomic DNA was extracted. A 152 bp fragment from exon 2 of the leptin gene was amplified. PCR products were digested with Hinf I restriction enzymes and was separated and visualized on the agarose gels. From possible three genotypes (TT, TC, and CC), only two genotypes TT and CC were observed in 14 studied domestic and wild populations with the genotype frequency of 95% and 5% respectively. The number of observed alleles, number of effective alleles, Nei’s Index and Shanon’s Index were 2, 1.10, 0.10 and 0.20 respectively. The studied populations were not found to be in Hardy- Weinberg equilibrium. Our investigation demonstrated that TT genotype and T allele had a very high frequency (0.95) in studied goats. Hence, it can be concluded that this finding can provide the basis for selection when considering evolution and differentiation among breeds, however, further studies should be carried out on a larger population of different domestic and wild breeds to verify the final conclusions.

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Published

30-06-2019

How to Cite

VAZIR, N. E. A. ., KOSHKOOIEH, A. E. ., MEHRJERDI, A. A., MOHAMMADABADI, M., BABENKO, O. I. ., BUSHTRUK, M. V., TKACHENKO, S. V., STAVETSKA, R. V., & KLOPENKO, N. I. (2019). COMPARISON OF GENETIC DIVERSITY OF LEPTIN GENE BETWEEN WILD GOAT AND DOMESTIC GOAT BREEDS IN IRAN. Malaysian Applied Biology, 48(3), 85–93. Retrieved from https://jms.mabjournal.com/index.php/mab/article/view/1843

Issue

Section

Research Articles