THERMAL DEATH EVALUATION OF MULTI-STRAINS PROBIOTIC INOCULANT FOR SHELF-LIFE PREDICTION

Authors

  • THANYARAT NAKSING Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • PRATCHAYA HATAWEE Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • APINAN WANLAPA Department of Agricultural Engineering, Faculty of Engineering Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • KITTIKOON TORPOL Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • CHIU-HSIA CHIU Department of Food Science, National Pingtung University of Science and Technology, Pingtung County 91201, Taiwan
  • JANTIMA TEEKA Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand; Center of Excellence in Nano-Biotechnology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand
  • ATSADAWUT AREESIRISUK Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand; Center of Excellence in Nano-Biotechnology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani 12110, Thailand

Keywords:

Arrhenius equation, probiotic, shelf-life prediction, thermal death

Abstract

This study aimed to determine the thermal death kinetic of Lactobacillus plantarum 7-40, Bacillus subtilis E20, and Saccharomyces cerevisiae P13 contained in probiotic product and to study the relation of specific death rate (kd) and temperature following the Arrhenius equation for shelf-life prediction. The result showed that the viability of probiotic strains was differently affected by storage temperature. The B. subtilis E20 could survival more than L. plantarum 7-40 and S. cerevisiae P13 under high storage temperature. It was also found that the lowest kd was provided by B. subtilis. This result demonstrated that the B. subtilis was more stable than L. plantarum and S. cerevisiae during keeping under high-temperature warehouse. Thereby, the storage temperature of the multi-strains probiotics inoculant should be considered carefully. Also, the natural logarithm of kd was well related to the storage temperature as the Arrhenius equation. The Arrhenius model could be applied for predicting the shelf-life of probiotic inoculant as well. This study could be used for managing the stock of a multi-strains probiotic product in the high-temperature warehouse.

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Published

30-11-2019

How to Cite

NAKSING, T., HATAWEE, P., WANLAPA, A., TORPOL, K., CHIU, C.-H., TEEKA, J., & AREESIRISUK, A. (2019). THERMAL DEATH EVALUATION OF MULTI-STRAINS PROBIOTIC INOCULANT FOR SHELF-LIFE PREDICTION. Malaysian Applied Biology, 48(4), 147–152. Retrieved from https://jms.mabjournal.com/index.php/mab/article/view/1889