A PRELIMINARY EVALUATION ON BIVARIATE ALLOMETRY IN ACTIVE-FEEDING Chrysomya megacephala (FABRICIUS, 1794) (DIPTERA: CALLIPHORIDAE) LARVAE

RAJA MUHAMMAD ZUHA

doi:10.55230/mabjournal.v51i1.1999

Authors

RAJA MUHAMMAD ZUHA
rmzuha@ukm.edu.my
Forensic Science Program, Faculty of Health Sciences, Basement One, Perpustakaan Tun Seri Lanang, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia; Centre for Insect Systematics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia; Fraser’s Hill Research Centre, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia https://orcid.org/0000-0003-0291-1388

Keywords:

forensic entomology, development, blow fly, correlation

Abstract

The potential use of larval cephalopharyngeal skeleton as an alternative growth indicator in forensic entomology practice was assessed based on its developmental pattern, growth performance and allometric relationship with larval body. Chrysomya megacephala (Fabricius, 1794), was used as an experimental species and larval development were studied at ambient temperatures and relative humidity. Larval body size was measured from furthest part of the head to the last abdominal segment. Cephalopharyngeal skeleton was extracted from the body and measured from the tip of dorsal bridge to the left face of dorsal cornu. Daily progression of larval body length and cephalopharyngeal skeleton length showed the latter significantly had slower growth rates. The allometry of larval body and cephalopharyngeal skeleton showed they were correlated only because both variables increased across the three larval instars, which suggesting a spurious correlation. Separate bivariate correlations between the two variables showed only the first instar larvae had a significant (p<0.01) but weak correlation, r=0.33. However, removing the effect of larval instars still produced statistically significant correlation (p<0.001) albeit with moderate strength, r=0.57. From this study, there was not enough evidence to support cephalopharyngeal skeleton as an equal or a better alternative to larval body as growth indicator.

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References

Abdullah, S.S. & Zuha, R.M. 2020. Cephalopharyngeal skeletons of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) third instar larvae displayed resistance to hot-water killing method – implications in forensic entomology practice. Malaysian Applied Biology, 49(5): 125-131. DOI: https://doi.org/10.55230/mabjournal.v49i5.1644

Adams, Z.J.O. & Hall, M.J.R. 2003. Methods used for the killing and preservation of blowfly larvae, and their effect on post-mortem larval length. Forensic Science International, 138:50-61. DOI: https://doi.org/10.1016/j.forsciint.2003.08.010

Amendt, J., Campobasso, C.P., Gaudry, E., Reiter, C., LeBlanc, H.N. & Hall, M.J.R. 2007 Best practice in forensic entomology-standards and guidelines. International Journal of Legal Medicine, 121(2):90-104. DOI: https://doi.org/10.1007/s00414-006-0086-x

Arnott, S. & Tuner, B. 2008. Post-feeding larval behaviour in the blowfly, Calliphora vicina: Effects on post-mortem interval estimates. Forensic Science International, 177(2-3): 162-167. DOI: https://doi.org/10.1016/j.forsciint.2007.12.002

Badenhorst, R. & Villet, M.H. 2018. The uses of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) in forensic entomology. Forensic Sciences Research, 3(1): 2-15. DOI: https://doi.org/10.1080/20961790.2018.1426136

Bai, Y., Dong, J.J., Guan, D.L., Xie, J.Y. & Xu, S.Q. 2016. Geographic variation in wing size and shape of the grasshopper Trilophidia annulata (Orthoptera: Oedipodidae): Morphological trait variations follow an ecogeographical rule. Scientific Reports, 6: 32680. DOI: https://doi.org/10.1038/srep32680

Bunchu, N., Thaipakdee, C., Vitta, A., Sanit, S., Sukontason, K. & Sukontason, K.L. 2012. Morphology and developmental rate of the blow fly, Hemipyrellia ligurriens (Diptera: Calliphoridae): Forensic entomology applications. Journal of Parasitology Research, 2012: 371243. DOI: https://doi.org/10.1155/2012/371243

Byrd, J.H. 2001. Laboratory rearing of forensic insects. In: Forensic Entomology: The Utility of Arthropods in Legal Investigations. J.H. Byrd and J.L. Castner (Eds.). CRC Press, Boca Raton. pp. 121-142. DOI: https://doi.org/10.1201/9781420036947.ch4

Catts, E.P. & Haskell, N.H. 1990. Entomology & Death: A Procedural Guide. Joyce’s Print Shop Inc., Clemson. 182 pp.

Chaiwat, C., Nateeworanart, S. & Jiraviriyakul, A. 2012. Length of cephalopharyngeal skeleton of Chrysomya rufifacies, third instar larvae, collected from Naresuan University, Phitsanulok Province. Forensic Medicine Journal, 4: 127-134.

Chen, W., Yang, L., Ren, L., Shang, Y., Wang, S. & Guo, Y. 2019. Impact of constant versus fluctuating temperatures on the development and life history parameters of Aldrichina grahami (Diptera: Calliphoridae). Insects, 10(7): 184. DOI: https://doi.org/10.3390/insects10070184

Clarkson, C.A., Hobischak, N.R. & Anderson, G.S. 2004. A comparison of the development rate of Protophormia terraenovae (Robineau-Desvoidy) raised under constant and fluctuating temperature regimes. Canadian Society of Forensic Science Journal, 37(2): 95-101. DOI: https://doi.org/10.1080/00085030.2004.10757567

Dadour, I.R., Cook, D.F., Fissioli, J.N. & Bailey, W.J. 2001. Forensic entomology: application, education and research in Western Australia. Forensic Science International, 120(1-2): 48-52. DOI: https://doi.org/10.1016/S0379-0738(01)00420-0

Day, D.M. & Wallman, J.F. 2006. Width as an alternative measurement to length for post-mortem interval estimations using Calliphora augur (Diptera: Calliphoridae) larvae. Forensic Science International, 159(2-3): 158-167. DOI: https://doi.org/10.1016/j.forsciint.2005.07.009

Day, D.M. & Wallman, J.F. 2008. Effect of preservative solutions on preservation of Calliphora augur and Lucilia cuprina larvae (Diptera: Calliphoridae) with implications for post-mortem interval estimates. Forensic Science International, 179(1): 1-10. DOI: https://doi.org/10.1016/j.forsciint.2008.04.006

Eliza, P. & Zuha, R.M. 2018. Preliminary assessment of cephalopharyngeal skeleton length and body length of Hemipyrellia ligurriens (Wiedemann) (Diptera: Calliphoridae) larvae as potential parameters to estimate minimum post mortem interval. Egyptian Journal of Forensic Sciences, 8: 39. DOI: https://doi.org/10.1186/s41935-018-0070-x

Ferrar, P. 1987. A guide to the breeding habits and immature stages of Diptera Cyclorrhapha. Entomonograph Volume 8. EJ Brill/Scandinavian Science Press, Leiden/Copenhagen. 907 pp.

Gennard, D.E. 2007. Forensic Entomology: An Introduction. John, Wiley & Sons Ltd., West Sussex. 272 pp.

Gomes, L., Godoy, W.A.C. & Von Zuben, C.J. 2006. A review of postfeeding larval dispersal in blowflies: implications for forensic entomology. Naturwissenschaften, 93(5): 207-15. DOI: https://doi.org/10.1007/s00114-006-0082-5

Greenberg, B. & Kunich, J.C. 2002. Entomology and the Law: Flies as Forensic Indicators. Cambridge University Press, Cambridge. 306 pp.

Kurahashi H., Benjaphong, N. & Omar, B. 1997. Blow flies (Insecta: Diptera: Calliphoridae) of Malaysia and Singapore. Raffles Bulletin of Zoology, Supplement Series No.5: 1-88.

Lawrence, P.O. 1979. Immature stages of the Carribean fruit fly, Anastrepha suspensa. Florida Entomologist, 62(3): 214-219. DOI: https://doi.org/10.2307/3494059

Lee, H.L., Krishnasamy, M., Abdullah, A.G. & Jeffery, J. 2004. Review of forensically important entomological specimens in the period of 1972-2002. Tropical Biomedicine, 21(2): 69-75.

Manlove, J. 2010. Forensic entomology. In: A Dipterist’s Handbook. Chandler, P. (Ed.), 2nd Ed. The Amateur Entomologist Society, Brentwood. pp. 181.

Nateeworanart, S., Bunchu, N. & Vitta, A. 2010. Cephalopharyngeal skeleton measurement of Chrysomya megacephala, third instar larvae, collected from Naresuan University, Phitsanuk Province. Journal of the Medical Technologist Association of Thailand, 38(1): 3100-3105.

Niederegger, S., Pastuschek, J. & Mall, G. 2010. Preliminary studies of the influence of fluctuating temperatures on the development of various forensically relevant flies. Forensic Science International, 199(1-3): 72-78. DOI: https://doi.org/10.1016/j.forsciint.2010.03.015

Nuñez, J.A. & Liria, J. 2016. Cephalopharyngeal geometric morphometrics in three blowfly species (Diptera : Calliphoridae). Journal of Entomology and Zoology Study, 4(1): 338-341.

Petitt, F.L. 1990. Distinguishing larval instars of the vegetable leafminer, Liriomyza sativae (Diptera: Agromyzidae). Florida Entomologist, 73(2): 280-286. DOI: https://doi.org/10.2307/3494812

Rabbani, A. & Zuha, R.M. 2017. Cephalopharyngeal skeleton morphometry of Hypopygiopsis violacea (Macquart) (Diptera: Calliphoridae) - A preliminary assessment for its application in forensic entomology. Journal of Entomology and Zoology Study, 5(1): 777-782.

Richards, C.S., Rowlinson, C.C. & Hall, M.J.R. 2013. Effects of storage temperature on the change in size of Calliphora vicina larvae during preservation in 80% ethanol. International Journal of Legal Medicine, 127(1): 231–241. DOI: https://doi.org/10.1007/s00414-012-0683-9

Roberts, M.J. 1971. The structure of the mouthparts of some calypterate dipteran larvae in relation to their feeding habits. Acta Zoologica, 52(2):171-188. DOI: https://doi.org/10.1111/j.1463-6395.1971.tb00556.x

Robinson, L.A., Bryson, D., Bulling, M.T., Sparks, N. & Wellard, K.S. 2018. Post-feeding activity of Lucilia sericata (Diptera: Calliphoridae) on common domestic indoor surfaces and its effect on development. Forensic Science International, 286:177-184. DOI: https://doi.org/10.1016/j.forsciint.2018.03.010

Rosilawati, R., Omar, B., Syamsa, R.A., Lee, H.L. & Nazni, W.A. 2014. Effects of preservatives and killing methods on morphological features of a forensic fly, Chrysomya megacephala (Fabricius, 1794) larva. Tropical Biomedicine, 31(4): 785-791.

Sim, L.X. & Zuha, R.M. 2019. Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae) development by landmark-based geometric morphometrics of cephalopharyngeal skeleton: a preliminary assessment for forensic entomology application. Egyptian Journal of Forensic Sciences, 9: 55. DOI: https://doi.org/10.1186/s41935-019-0158-y

Simon, P.P., Krüger, R.F. & Ribeiro, P.B. 2011. Influence of diets on the rearing of predatory flies of housefly larvae. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63(6): 1414-1420. DOI: https://doi.org/10.1590/S0102-09352011000600019

Smith, K.G.V. 1986. A Manual of Forensic Entomology. British Museum (Natural History), New York. 205 pp.

Sukontason, K., Piangjai, S., Siriwattanarungsee, S. & Sukontason, K.L. 2008. Morphology and developmental rate of blowflies Chrysomya megacephala and Chrysomya rufifacies in Thailand: Application in forensic entomology. Parasitol Research, 102(6): 1207-1216. DOI: https://doi.org/10.1007/s00436-008-0895-6

Sukontason, K., Sukontason, K., Vichairat, K., Piangjai, S., Lertthamnongtham, S., Vogtsberger, R.C. & Olson, J.K. 2001. The first documented forensic entomology case in Thailand. Journal of Medical Entomology, 38(5): 746-748. DOI: https://doi.org/10.1603/0022-2585-38.5.746

Syamsa, R.A., Omar, B., Ahmad, F.M.S., Hidayatulfathi, O. & Shahrom, A.W. 2017. Comparative fly species composition on indoor and outdoor forensic cases in Malaysia. Journal of Forensic and Legal Medicine, 45: 41-46. DOI: https://doi.org/10.1016/j.jflm.2016.12.002

Tantawi, T.I. & Greenberg, B. 1993. The effect of killing and preservative solutions on estimates of maggot age in forensic cases. Journal of Forensic Sciences, 38(3): 702-707. DOI: https://doi.org/10.1520/JFS13458J

Teskey, H.J. 1981. Morphology and terminology - Larvae. In: Manual of Nearctic Diptera. J.F. McAlpine, B.V. Peterson, G.E. Shewell, H.J. Teskey, J.R. Vockeroth & D.M. Wood (Eds). Canadian Government Publishing Centre, Quebec. pp .65-88.

Thevan, K., Ahmad, A.H., Md Rawi, C.S. & Singh, B. 2010. Growth of Chrysomya megacephala (Fabricius) maggots in a morgue cooler. Journal of Forensic Sciences, 55(6): 1656-1658. DOI: https://doi.org/10.1111/j.1556-4029.2010.01485.x

Villet, M.H., Richards, C.S. & Midgley, J.M. 2010. Chapter 7 Contemporary, precision, bias and accuracy of minimum post-mortem intervals estimated using development of carrion-feeding insects. In: Current Concepts in Forensic Entomology. J. Amendt, C.P. Campobasso, M.L. Goff, M. Grassberger (Eds.). Springer, Dordrecht. pp. 109-137. DOI: https://doi.org/10.1007/978-1-4020-9684-6_7

Warren, J-A. & Anderson, G.S. 2013. Effect of fluctuating temperatures on the development of a forensically important blow fly, Protophormia terraenovae (Diptera: Calliphoridae). Environmental Entomology, 42(1): 167-172. DOI: https://doi.org/10.1603/EN12123

Zuha, R.M. 2021. Effects of preservative concentrations on larval cephalopharyngeal skelton of Chrysomya megacephala (Fabricius, 1794) (Diptera: Calliphoridae), an alternative indicator to larval body length for mPMI estimation. Journal of Clinical and Health Sciences, 6(1): 90–101. DOI: https://doi.org/10.24191/jchs.v6i1(Special).13992