BIOACTIVITY OF BLACK CUMIN OIL ON THE SENESCENCE OF HER-2-OVEREXPRESSING BREAST CANCER CELLS

FARADIBA NUR AHLINA; LISYARATIH ANGGRIANI; IRFANI AURA SALSABILA; RIRIS ISTIGHFARI JENIE

doi:10.55230/mabjournal.v51i1.2008

Authors

FARADIBA NUR AHLINA Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia https://orcid.org/0000-0002-4353-4200
LISYARATIH ANGGRIANI Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
IRFANI AURA SALSABILA Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
RIRIS ISTIGHFARI JENIE
riris_jenie@ugm.ac.id
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia https://orcid.org/0000-0002-0230-6260

Keywords:

black cumin oil, MCF7/HER2 cell, senescence, p-cymene, breast cancer

Abstract

Senescence-induced therapy has been improved to increase its cytotoxicity and reduce the resistance of breast cancer cells to chemotherapy agents. An example of a potential senescence-inducing agent is black cumin oil (BCO) because one of its major compounds, α-pinene, can induce senescent cells. This study aims to explore the senescence-inducing activity of BCO in HER2-overexpressing breast cancer cells (MCF7/HER2). The yield obtained from hydro-distillation of BCO was 0.54%, and the main compounds were p-cymene (48.03%), dihydrocarveol (11.39%), and α-pinene (11.29%). BCO exhibited a moderate cytotoxicity profile indicated by IC₅₀, which was >200 μg/mL in both cell lines. In combination with doxorubicin, BCO did not increase the cytotoxicity of doxorubicin. Moreover, BCO induced senescence by increasing 3% of the senescent cells compared with that of the control cells. However, this result was lower than that of the positive control on MCF7/HER2. BCO and doxorubicin combination increased the senescent cells by 3%–7% compared with the positive control on MCF7/HER2 cells. Therefore, the moderate cytotoxicity of BCO could be beneficial to the application of BCO as a supportive agent combined with a chemotherapy drug to increase cancer cells senescent and consequently inhibit cell proliferation.

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