ANTIOXIDANT CAPABILITIES OF Litsea garciae BARK EXTRACTS AND THEIR RELATION TO THE PHYTOCHEMICAL COMPOSITIONS

SITI ZALEHA RADUAN; QAMAR UDDIN AHMED; ABDUL RAZAK KASMURI; MUHAMAD RUSDI AHMAD RUSMILI; MD ABDUR RASHID MIA; WAN MOHD AZIZI WAN SULAIMAN; MUHAMMAD HAMDI MAHMOOD; MOHD FAROOQ SHAIKH

doi:10.55230/mabjournal.v51i1.2038

Authors

SITI ZALEHA RADUAN Department of Basic Medical Sciences, Kulliyyah of Pharmacy, International Islamic University of Malaysia; Department of Paraclinical Sciences, Universiti Malaysia Sarawak https://orcid.org/0000-0003-1260-9537
QAMAR UDDIN AHMED
quahmed@iium.edu.my
Department of Pharmaceutical Chemistry, Kulliyyah of Pharmacy, International Islamic University of Malaysia https://orcid.org/0000-0003-0565-3222
ABDUL RAZAK KASMURI Department of Basic Medical Sciences, Kulliyyah of Pharmacy, International Islamic University of Malaysia https://orcid.org/0000-0003-4774-0535
MUHAMAD RUSDI AHMAD RUSMILI Department of Basic Medical Sciences, Kulliyyah of Pharmacy, International Islamic University of Malaysia
MD ABDUR RASHID MIA Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University of Malaysia https://orcid.org/0000-0002-0954-4501
WAN MOHD AZIZI WAN SULAIMAN Faculty of Health Sciences, PICOMS International University College
MUHAMMAD HAMDI MAHMOOD Department of Preclinical Sciences, Universiti Malaysia Sarawak
MOHD FAROOQ SHAIKH Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia https://orcid.org/0000-0001-9865-6224

Keywords:

hexane, chloroform, methanol, aqueous, antioxidant, Litsea garcia

Abstract

The plant species belonging to the Litsea genus are widely investigated due to their nutritional and medicinal purposes. In this regard, this study is another similar sincere effort in which the antioxidant property and phytochemical composition of Litsea garciae (L. garciae) bark’s hexane, chloroform, methanol, and aqueous extracts were evaluated to confirm its traditional benefits. The total flavonoid content (TFC) and total phenolic content (TPC) were determined first, followed by an assessment of in vitro antioxidant activity using the DPPH and FRAP assays. The composition of the secondary metabolites was determined using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UHPLC-MS). As a result, methanol extract was recorded to have the highest TPC value aligned with its positive appearance in phytochemical screening. Its antioxidant capacity indicated the least IC₅₀. The results indicated that the significant free radical scavenging activity was due to the methanolic extract's high phenolic content. The secondary metabolites found in the methanol extract varied significantly according to UHPLC-MS analysis. The major phenolic compounds were found including N-trans-feruloyl-4-O-methyldopamine, N-cis-feruloyltyramine, epicatechin-(4beta->6)-epicatechin-(2beta->7,4beta->8)-epicatechin, 7-Hydroxy-3-(4-methoxyphenyl)-4-propyl-2H-1-benzopyran-2-one and 9-O-Methylneodunol. In general, the results indicate that L. garciae bark may be a promising source of novel natural compounds with antioxidative properties.

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Author Biography

MD ABDUR RASHID MIA, Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, International Islamic University of Malaysia

Department of Pharmaceutical Technology, Kulliyyah of Pharmacy, IIUM

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