DNA PROFILE OF COMMERCIAL PINEAPPLES IN MALAYSIA BY USING SSR MARKERS

Authors

  • SITI NORHAYATI ISMAIL Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), MARDI headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia
  • NURUL SHAMIMI ABDUL GHANI Horticulture Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), MARDI Pontian, KM 53, Jalan Johor, 82000 Pontian, Johor, Malaysia
  • SHAHRIL AB RAZAK Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), MARDI headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia
  • RABIATUL ADAWIAH ZAINAL ABIDIN Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), MARDI headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia
  • MOHD NIZAM ZUBIR Horticulture Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), MARDI Pontian, KM 53, Jalan Johor, 82000 Pontian, Johor, Malaysia
  • ROZLAILY ZAINOL Industrial Crop Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), MARDI headquarters, Persiaran MARDI-UPM, 43400 Serdang, Selangor, Malaysia

Keywords:

commercial pineapple varieties, genetic identification, heterozygosity, simple sequence repeat (SSR)

Abstract

Nine commercial varieties of pineapples in Malaysia (Josapine, Maspine, MD2, Sarawak, Gandul, N36, Moris, Crystal Honey and Yankee) were collected from various places in Peninsular Malaysia and analysed for cultivar identifications using nine simple sequence repeat (SSR) markers. A total of 27 alleles have been observed which ranged from 2 to 5 with an average of 3 alleles per locus. The polymorphic information content (PIC) value ranged from 0.3426 (Acom_82.8) to 0.6561 (Acom_67.2) with a mean of 0.4524 while the heterozygosity value ranged from 0.1097 (TsuAC021) to 0.8010 (TsuAC039) with a mean of 0.5481. The pairwise Nei’s genetic distances had also been calculated and the value ranged from 0.0562 (Gandul and Josapine) to 0.6383 (MD2 and Yankee) with an average value of 0.3169. The above data emphasised a moderate level of polymorphisms among the nine varieties. A dendrogram was constructed by using the unweighted pair group method with arithmetic mean (UPGMA) which showed all the nine successfully differentiated pineapple commercial varieties. A principal coordinate analysis (PCoA) was also had been generated which revealed an agreement with the dendrogram output. Therefore, these nine SSR markers can be used to identify the nine selected commercial varieties to ensure pure planting materials.

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Published

31-12-2021

How to Cite

ISMAIL, S. N., ABDUL GHANI, N. S. ., RAZAK, S. A., ABIDIN, R. A. Z., ZUBIR, M. N., & ZAINOL, R. (2021). DNA PROFILE OF COMMERCIAL PINEAPPLES IN MALAYSIA BY USING SSR MARKERS. Malaysian Applied Biology, 50(3), 15–22. Retrieved from https://jms.mabjournal.com/index.php/mab/article/view/2209

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Section

Research Articles