EVALUATIONS OF ENTOMOPATHOGENIC FUNGI, Metarhizium anisopliae INOCULATE ON THE TREATED SOILS TOWARDS Paederus fuscipes

ANDY FU HAO TAN; HIDEYUKI NAGAO; WAN FATMA  ZUHARAH

doi:10.55230/mabjournal.v51i1.2264

Authors

ANDY FU HAO TAN School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
HIDEYUKI NAGAO School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
WAN FATMA ZUHARAH
wanfatma@gmail.com
School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; Vector Control Research Unit, School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia https://orcid.org/0000-0002-5789-2244

Keywords:

Entomopathogenic, Metarhizium anisopliae, Paederus fuscipes, pathogenicity

Abstract

Rove beetle, Paederus fuscipes Curtis is a natural predator of several crop pests in the agriculture ecosystem, however, their high intrusion into human settlements caused them to become public health concern due to Paedarus dermatitis infection among humans. The entomopathogenic effectiveness of Metarhizium anisopliae Mechnikov was tested as biological control towards adults Paederus fuscipes by inoculating on soils. The mortality of P. fuscipes was observed and data were subjected to analysis using ANOVA and Kaplan-Meier method. Results show that P. fuscipes tested with the highest concentration at 1.3 × 10¹⁰ conidia/mL exhibited the shortest mean mortality time at 11.0 ± 2.5 days and survival time of 7.6 ± 0.7 days, yet the second-highest concentration exhibited at 2.2 × 10⁹ showed mean mortality of 18.4 ± 4.2 days and survival time of 11.9 ± 0.8 days. Log Rank (Mantel-Cox) pairwise comparison indicated the significant differences between the highest concentration of 1.3 × 10¹⁰with the control (χ²= 62.3, df=1 p<0.0005). Both mean mortality time and survival time of P. fuscipes showed inconsistent trends from the highest concentration of M. anisopliae towards the lowest. Pathogenicity was observed at the concentrations of 10⁶, 10⁹, and 10¹⁰ after performing Koch’s postulates. The results were unexpected but could indicate that M. anisopliae has the potential to be a biocontrol agent at a higher concentration.

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