EXPRESSION OF BCL-2 AND BAX GENES INDUCED BY AN OVARIAN EXTRACT OF SINGKARAK LAKE PUFFERFISH (Tetraodon leiurus) IN BREAST CANCER CELL

MONICA MULNIA HANIF; DJONG  HON TJONG; SYAIFULLAH; PUTRA  SANTOSO; EFRIZAL; DEWI IMELDA ROESMA

doi:10.55230/mabjournal.v51i3.2283

Authors

MONICA MULNIA HANIF Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia
DJONG HON TJONG Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia
SYAIFULLAH Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia
PUTRA SANTOSO Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia
EFRIZAL Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia
DEWI IMELDA ROESMA
dewiroesma@sci.unand.ac.id
Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia

Keywords:

Cancer, chemoprevention, Jabuih fish, Lake Singkarak, MCF-7 cell line

Abstract

It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has the potential for chemoprevention in MCF-7 cells. However, the expression of Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze the Bcl-2 and Bax gene expression induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in MCF-7. The method in this study was an in vitro experiment using MCF-7 control cells and MCF-7 cells induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus), and Real-time PCR was used to examine the expression of Bcl-2 and Bax genes. The result showed that the expression of the Bcl-2 gene had an insignificant decrease (p>0.05) by 15%, and the expression of the Bax gene had a significant increase (p<0.05) by 489% compared to the control. These results support that ovarian extract Singkarak Lake Pufferfish (Tetraodon leiurus) can be alternative cancer chemoprevention.

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Author Biographies

DJONG HON TJONG, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia

Biology Department

EFRIZAL, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University, Limau Manis, Padang, West Sumatra, 25163, Indonesia

Biology Department

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