A PRELIMINARY STUDY OF PHYTOCHEMICAL CONTENTS IN COMMERCIAL MALAYSIAN BLACK TEA EXTRACT USING QUANTITATIVE AND QUALITATIVE TESTS
Keywords:Organic extracts, black tea, phytochemical, phenolic, flavonoid
Black tea (Camellia sinensis) is a flavoured, functional, and therapeutic non-alcoholic drink that is consumed by two-thirds of the world’s population. In this study, the bioactive compounds in black tea leaves were extracted using two different solvents which are methanol and acetone before being further characterized with FTIR to identify the functional groups. The presence of active compounds such as gallic tannin, catecholin tannin, phenol and flavonoid compounds were further tested with qualitative and quantitative methods. Based on FTIR results, the best solvent to extract tea leaves was acetone. The frequency obtained for phenolic –OH stretch was 3390.5 cm-1, while alkane –CH3 stretch appeared at 2921.4 cm-1 and 2850.7 cm-1. The UV-Vis spectrum was obtained and the λ max from the spectrum is 270 nm. Qualitative analysis involving ferric chloride and lead acetate test showed positive results for the presence of gallic tannin, catecholin, flavonoid and phenols in the tea leaves extract. From the quantitative test, the total phenolic content was 0.9217 mg gallic acid equivalent (GAE)/L while the concentration of flavonoid content was 0.7792 mg quercetin/L and total tannin content 0.13 g/g respectively.
Abidin, Z.H.Z., Naziron, N.N., Nasir, K.M., Rusli, M.S., Lee, S.V., Kufian, M.Z. & Yahya, R. 2013. Influence of curcumin natural dye colorant with PMMAacrylic polyol blended polymer. Pigment & Resin Technology, 42(2): 95-102.
Bizuayehu, D., Atlabachew, M. & Ali, M. 2016. Determination of some selected secondary metabolites and their invitro antioxidant activity in commercially available Ethiopian tea (Camellia sinensis). Springerplus, 5(1): 1-9.
Bhutia Pemba, H., Sharangi Baran, A., Lepcha, R. & Tamang, D. 2015. Bioactive Compounds and Antioxidant Properties of Tea: Status, Global Research and Potentialities. Journal of Tea Science Research, 5(11): 1-13.
Dwyer, J., Holden, J. & Peterson, J. 2018. Flavonoid composition of tea: Comparison of black and green teas. Agriculture food service. Indexmundi.com. 2018. Tea - Monthly Price (Malaysian Ringgit per Kilogram) - Commodity Prices - Price Charts, Data, and News -IndexMundi.
Kalita, P., Tapan, B., Pal, T. & Kalita, R. 2013. Estimation of Total Flavonoids Content (Tfc) and Anti Oxidant Activities of Methanolic Whole Plant Extract of Biophytum Sensitivum Linn. Journal of Drug Delivery and Therapeutics, 3(4): 34-37.
Liebert, M., Licht, U., Böhm, V. & Bitsch, R. 1999. Antioxidant properties and total phenolics content of green and black tea under different brewing conditions, Zeitschrift für Lebensmitteluntersuchung und-Forschung A, 208(3): 217-220.
Loto, C.A. 2011. Inhibition effect of tea (Camellia Sinensis) extract on the corrosion of mild steel in dilute sulphuric acid. Journal of Materials and Environmental Science, 2(4): 335-344.
Mohd Zain, S. & Wan Omar, W. 2018. Antioxidant Activity, Total Phenolic Content and Total Flavonoid Content of Water and Methanol Extracts of Phyllanthus species from Malaysia. Pharmacognosy Journal, 10(4): 677-681.
Nibir, Y., Sumit, A., Akhand, A., Ahsan, N. & Hossain, M. 2017. Comparative assessment of total polyphenols, antioxidant and antimicrobial activity of different tea varieties of Bangladesh. Asian Pacific Journal of Tropical Biomedicine, 7(4): 352-357.
Pisoschi, A.M. & Negulescu, G.P. 2011. Methods for total antioxidant activity determination: A review. Biochemistry and Analytical Biochemistry, 106: 1.
Qian, B., Hou, B. & Zheng, M. 2013. The inhibition effect of tannic acid on mild steel corrosion in seawater wet / dry cyclic conditions. 72: 1-9.
Sangeetha, J. & Vijayalakshmi, K. 2011. Determination of Bioactive Components of Ethyl Acetate Fraction of Punica granatum Rind Extract. International Journal of Pharmaceutical Sciences and Drug Research, 3(2): 116-122.
Sentilkumar, S., Sivakumar, T., Arulmozhi, K. & Mythili, N. 2017. FT-IR analysis and correlation studies on the antioxidant activity, total phenolics and total flavonoids of Indian commercial teas (Camellia sinensis L.) – A novel approach. International Research Journal of Biological Science, 6(3): 1-7.
Sharangi, A.B., Siddiqui, M.W. & Avina, J.D. 2014. Black tea magic: overview of global research on human health and therapeutic potentialities. Journal of Tea Science Research, 4(1): 1-10.
Stankovic, M.S. 2011. Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts. Kragujevac Journal of Sciences, 33(2011): 63-72.
Tariq, A.L. & Reyaz, A.L. 2012. Phytochemical analysis of Camellia sinensis Leaves. International Journal of Drug Development and Research, 4(4): 311-316.
Uddin, N., Siddiqui, B.S., Begum, S., Bhatti, H.A., Khan, A., Parveen, S. & Choudhary, M.I. 2011. Bioactive flavonoids from the leaves of Lawsonia alba (Henna). Phytochemistry Letters, 4(4): 454-458.
Velioglu, Y., Mazza, G., Gao, L. & Oomah, B. 1998. Antioxidant Activity and Total Phenolics in Selected Fruits, Vegetables, and Grain Products. Journal of Agricultural and Food Chemistry, 46(10): 4113-4117.
Verwoerd, R. & L. Facenda, V. 2013. Discovering Tea in Malaysia | World Tea News. [online] Worldteanews.com. Available at: https://worldteanews.com/tea-estate-and-garden-profiles/discovering-tea-malaysia.
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