EPIGENETIC MODIFIERS AND MINERALS AS TOOLS TO DIVERSIFY SECONDARY METABOLITE PRODUCTION IN FUNGI

SITI HAJAR SADIRAN; FATMAWATI LAMBUK; SITI NUR SARAH  ZUBIR; RASHA SAAD  SULIMAN; JEAN-FRÉDÉRIC FAIZAL  WEBER

doi:10.55230/mabjournal.v51i4.20

Authors

SITI HAJAR SADIRAN
hajarsadiran@uitm.edu.my
Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA (Selangor Branch), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia; School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, Malaysia. https://orcid.org/0000-0001-6699-4464
FATMAWATI LAMBUK Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA (Selangor Branch), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia; Faculty of Pharmacy, Universiti Teknologi MARA (Selangor Branch), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
SITI NUR SARAH ZUBIR Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA (Selangor Branch), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia; Faculty of Pharmacy, Universiti Teknologi MARA (Selangor Branch), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia.
RASHA SAAD SULIMAN Department of Pharmacy, Fatima College of Health Scienes, P.O. Box 42162, Al Maqam, Al Ain, United Arab Emirates
JEAN-FRÉDÉRIC FAIZAL WEBER Atta-ur-Rahman Institute for Natural Product Discovery (AuRIns), Universiti Teknologi MARA (Selangor Branch), Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia; Laboratoire de Pharmacognosie, UFR Sciences Pharmaceutiques, Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux cedex, France

Keywords:

epigenetic modifier, elicitors, secondary metabolites, soil fungi

Abstract

Secondary metabolite production of fungi can be modified by different approaches, including epigenetic modifiers, culture-dependent methods, and genomic-based methods. In this study, secondary metabolite production was explored in the presence of epigenetic modifiers and minerals using a microscale fermentation approach. Thirteen fungi originally isolated from mushrooms and soils were grown in 96-well microtiter plates (MTPs) using 70% of potato dextrose broth (PDB) with the addition of epigenetic modifiers and minerals in different combinations and concentrations. All cultures were fermented at 10 °C or 28 °C for 2, 3, or 5 weeks and extracted by solid phase extraction. The resulting extracts were subjected to high-performance liquid chromatography (HPLC) and the chromatograms were analyzed on a qualitative and quantitative basis. In addition, major secondary metabolites from four fungi were identified as penicillic acid, patulin, pseurotin A, and javanicin. Epigenetic modifiers and minerals induce significant changes in the profile of the secondary metabolites. Their usage combined with microscale fermentation provides a cost-efficient tool for exploring fungal secondary metabolism.

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