Seed Longevity of Soybean (Glycine max L.) Cultivars Under Lightless Storage


  • Kadapi Muhamad Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Indonesia
  • Kezia Nauli Agrotechnology Study Program, Faculty of Agriculture, Universitas Padjadjaran, Indonesia
  • Fiky Yulianto Wicaksono Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Indonesia
  • Anas Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Indonesia


deteriotation, seed quality, soybean, vigour, light


Soybean seeds in tropical countries are prone to rapid deterioration due to environmental condition during storage, leading to a decrease in seed quality and ultimately resulting in low potential yield. Longevity of seed during storage is influenced by genetic and environmental factors such as light. Therefore, this study aimed to determine the effect of light on the vigour of two soybean seeds cultivars (Demas 1 & Devon 1 cvs) stored for three months. The investigation was conducted from February to May 2022 at Seed Technology Laboratory, Faculty of Agriculture, Universitas Padjadjaran. A correlational method was employed to assess the relationship between seed quality of Demas 1 and Devon 1 in light and lightless storage. Furthermore, data were analyzed using correlation regression and t-test to compare the two populations. The results showed that there was a high correlation between the physical and physiological quality of seeds. There were also differences in seed response to light, and this was observed from the moisture content, weight of 100 seeds, germination rate, and simultaneous growth percentage traits. Considering the seed quality traits, Demas 1 had the best vigour value after storage in dark conditions. It is worth noting that both cultivars exhibited signs of deterioration during storage. However, the rate of deterioration was slowed down due to lightless condition.


Download data is not yet available.


Metrics Loading ...


Abdalla F.H. & Roberts E.H. 1968. Effects of temperature, moisture, and oxygen on the induction of chromosome damage in seeds of barley, broad beans, and peas during storage. Annals of Botany, 32: 119-136. DOI:

Alemu, G. 2019. Review on the effect of seed source and size on grain yield of bread wheat (Tritium aestivum L.). Journal of Ecology & Natural Resources, 3(1): 1-8. DOI:

Ballesteros, D., Pritchard, H.W., & Walters, C. 2020. Dry architecture: Towards the understanding of the variation of longevity in desiccation-tolerant germplasm. Seed Science Resources. 30:142-155. DOI:

BPS (Badan Pusat Statistik). 2017. Kajian Konsumsi Bahan Pokok [Staple Food Consumption Study].

BPS. 2020. Impor kedelai menurut negara asal utama [WWW Document]. BPS. URL (accessed 5.19.23).

Chandra, S., Taak, Y., Rathod, D.R., Yadav, R.R., Poonia, S., Sreenivasa, V. & Talukdar, A. 2020. Genetics and mapping of seed coat impermeability in soybean using inter-specific population. Physiology and Molecular Biology of Plants, 26(11): 2291-2299. DOI:

Chidananda, K.P., Chelladurai, V., Jayas, D.S., Alagusundaram, K., White, N.D.G. & Fields, P.G. 2014. Respiration of pulses stored under different storage conditions. Journal of Stored Products Research, 59(2014): 42-47. DOI:

De Vitis, M., Hay, F.R., Dickie, J. B., Trivedi, C. & Fiegener, R. 2020. Seed storage: Maintaining seed viability and vigour for restoration use. Restoration Ecology, 28(S3): S249-S255. DOI:

FAO. 2023. Soybean. [WWW Document]. FAO. URL databases-and-software/crop-information/soybean/en (accessed 6.19.23).

Groot, A.P.C., Surki, A.A., De Vos, R.C.H. & Kodde, J. 2012. Seed storage at elevated partial pressure of oxygen, a fast method for analysing seed ageing under dry conditions. Annals of Botany, 110 (6): 1149-1159. DOI:

Hampton, J. 2002. What is seed quality? Seed Science and Technology 30(1):1-10.

Harrington, J.F. 1972. Seed storage and longevity. In: Seed Biology. T.T. Kozlowski (Ed.). New York (US): Academic Press. DOI:

ISTA (International Seed Testing Association). 2015. Seed Science and Technology. International Rules for Seed Testing, Zurich (CH). International Seed Testing Association.

Jawak, G., Widajati, E., Liana, D. & Astuti, T. 2022. Pendugaan kemunduran benih dengan uji fisiologi dan biokimiawi. Savana Cendana, 7(04): 61-64. DOI:

Khan, F.A., Bhar, S.A., Narayan, S., Mawbool, R., Murtuza, I. & Khan, F.U. 2017. Seed deterioration and priming-an overview. SKUAST Journal of Research, 19(1):12-21.

Koskosidis, A., Khah, E.M., Pavli, O.I. & Vlaschostergios, D.N. 2022. Effect of storage conditions on seed quality of soybean (Glycine max L.) Germplasm. AIMS Agriculture and Food, 7(2): 387-402. DOI:

Mahjabin, Bilal, S. & Abidi, A.B. 2015. Physiological and biochemical changes during seed deterioration: A review. International Journal of Recent Scientific Research, 6(4). 3416-3422.

Marwanto, M. 2007. Hubungan antara kandungan lignin kulit benih dengan sifat-sifat khusus kulit benih kacang hijau. Jurnal Ilmu-Ilmu Pertanian Indonesia, 9(1): 6-11. DOI:

Mbofung, G.Y. 2012. Effects of maturity group, seed composition and storage conditions on the quality and storability of soybean (Glycine max L. Merrill) seed. Iowa State University.

Muhamad, K., Denny Sobardini, D., Helena, E., Hanindianingrum, H., Noor, F., Wicaksana, W. & Sumadi. 2021. Allelopathic effect of west java local black rice varieties on barnyard grass (Echinochloa crus-galli (L.) Beauv.) at germination stage. Current Applied Science and Technology, 21(4): 673-685.

Noviana, I., Qadir, A. & Suwarno F.C. 2016. Perilaku biokimia benih kedelai selama penyimpanan dalam kondisi terkontrol. Jurnal Agron Indonesia, 44(3). 255-260. DOI:

Qu, S., Kwon, S.J., Duan, S., Lim, Y.J. & Eom, S.H. 2021. isoflavone changes in immature and mature soybeans by thermal processing. Molecules, 26(24): 7471. DOI:

Rahajeng, W. & Hapsari, R.T. 2018. Kualitas mutu benih lima varietas kedelai pada beberapa periode simpan. In: Prosiding Membangun Kemandirian Korporasi Petani Indonesia Menuju Kedaulatan Pangan Berkelanjutan. Sekolah Tinggi Penyuluhan Pertanian, Malang. pp 15-26.

Rizaty, M.A. 2022. Proyeksi luas panen kedelai RI terus menurun sampai 2024. [WWW Document]. Databoks]. URL luas-panen-kedelai-ri-terus-menurun-sampai-2024 (accessed 5.6.23).

Scariot, M.A., Soares, G.C., Radünz, L.L. & Dionello, R.G. 2021. Physical and physiological quality ofrice seeds in function of drying temperature and storage. Revista Ceres, 68: 31-38. DOI:

Sundareswaran, S., Ray Choudhury, P., Vanitha, C. & Yadava, D.K. 2023. Seed quality: Variety development to planting-An overview. In: Seed Science and Technology. M. Dadlani and D.K. Yadava (Eds.). Springer, Singapore. DOI:

Tatipata, A., Yudono, P., Purwantoro, A. & Mangoendidjojo, W. 2004. Kajian aspek fisiologi dan biokimia deteriorasi benih kedelai dalam penyimpanan. Ilmu Pertanian, 11(2): 76-87.

Tefa, A. 2017. Uji viabilitas dan vigour benih padi (Oryza sativa L.) selama penyimpanan pada tingkat kadar air yang berbeda. Savana Cendana, 2(3): 48-50. DOI:

Vertucci, C.W. & Leopold, A.C. 1987. Oxidative processes in soybean and pea seeds: Effect of light, temperature and water content. Plant Physiology, 84: 1038-1043. DOI:

Wahyuni, A., Putri, R., Jumawati, R. & Prasinta, F.P. 2022. Evaluasi mutu fisiologis benih kedelai kuning (Glycine max L.) pada penyimpanan terbuka. Jurnal Agrotek Tropika, 10(4): 555-562. DOI:

Wu, Yu-Z., Lu, H-M., Chen, L., Yang, F-Y., Mou, C-C. & Luan, L-l. 2019. Effect of oxygen content and light on fat oxidation during storage of coix seeds. Modern Food Science and Technology, 35(5): 109-116.



How to Cite

Muhamad, K., Nauli, K., Wicaksono, F. Y., & Anas. (2024). Seed Longevity of Soybean (Glycine max L.) Cultivars Under Lightless Storage. Malaysian Applied Biology, 53(2), 47–54.



Research Articles

Funding data