Genome Editing for Enhanced Abiotic Stress Tolerance in Selected Cereal (Poaceae) Crops: Current Applications, Tools, and Future Perspectives

Amin-Asyraf  Tamizi; Rabiatul-Adawiah Zainal-Abidin; Anis Afuza Md-Yusof; Nurul Asyikin Mohd-Zim; Mohd Syahmi Salleh; Nur Sabrina Ahmad Azmi; Zarina Zainuddin; Sarahani Harun; Rogayah Sekeli; Nurul Hidayah Samsulrizal

doi:10.55230/mabjournal.v54i4.3226

Authors

Amin-Asyraf Tamizi Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia; Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), 43400 Serdang, Selangor, Malaysia
Rabiatul-Adawiah Zainal-Abidin Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), 43400 Serdang, Selangor, Malaysia
Anis Afuza Md-Yusof Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia
Nurul Asyikin Mohd-Zim Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia; FGV R&D Sdn. Bhd., FGV Innovation Centre (Biotechnology), PT. 23417 Lengkuk Teknologi, 71760 Bandar Enstek, Negeri Sembilan, Malaysia
Mohd Syahmi Salleh Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia
Nur Sabrina Ahmad Azmi Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia; Plant Productivity and Sustainable Resource Unit, Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia
Zarina Zainuddin Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia; Plant Productivity and Sustainable Resource Unit, Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia
Sarahani Harun Centre for Bioinformatics Research, Institute of Systems Biology, Universiti Kebangsaan Malaysia (UKM), 43600 Bangi, Selangor, Malaysia
Rogayah Sekeli Biotechnology and Nanotechnology Research Centre, Malaysian Agricultural Research and Development Institute (MARDI), 43400 Serdang, Selangor, Malaysia
Nurul Hidayah Samsulrizal
hidayahsamsulrizal@iium.edu.my
Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia; Plant Productivity and Sustainable Resource Unit, Department of Plant Science, Kulliyyah of Science, International Islamic University Malaysia (IIUM), 25200 Kuantan, Pahang, Malaysia https://orcid.org/0000-0002-0019-0604

Keywords:

Abiotic stress, climate change, crop improvement, omics, plant biotechnology, staple food

Abstract

Recent progress in genome editing (GEd) technology offers an opportunity to accelerate the breeding of improved crops with enhanced resistance and high tolerance to drought and salinity. In this article, we highlight four programmable site-specific nucleases that are considered prominent GEd technologies: meganucleases, zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) nucleases. We then focus on the application of CRISPR/Cas9 system and access the transformation methods that have been used to deliver the system into major cereal crops including rice (Oryza sativa), maize (Zea mays), barley (Hordeum vulgare), sorghum (Sorghum bicolor), and wheat (Triticum aestivum). This review further emphasises the applications of the CRISPR/Cas9 system to impart tolerance to two major abiotic stresses, salinity and drought, in these selected crops. Finally, we summarise bioinformatics tools that are available for cereal genome editing works, including guide RNA (gRNA) design and post-editing analysis tools. This review provides an overview of current progress, identifies research gaps, and offers perspectives for prospective scientists embarking on genome editing in cereals and related crops.

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