Using Elicitors to Induce Curcuminoid Biosynthesis in Curcuma longa Via In Vitro Micropropagation

Parisatcha Sangsuwan; Oranual  Hanmeng; Wijit  Wisoram

doi:10.55230/mabjournal.v55i1.3663

Authors

Parisatcha Sangsuwan
parisatcha.s@lawasri.tru.ac.th
Science Department, Faculty of Science and Technology, Thepsatri Rajabhat University, Lopburi, 15000
Oranual Hanmeng Science Department, Faculty of Science and Technology, Thepsatri Rajabhat University, Lopburi, 15000
Wijit Wisoram Rajamangala University of Technology Tawan-ok, Department of Science and Mathematics, Faculty of Science and Technology, Chon Buri, Thailand, 20110

Keywords:

Curcuma longa, curcuminoid, somatic embryogenesis, elicitor, growth retardation, microrhizome

Abstract

Turmeric (Curcuma longa) is an important herbal plant used in the cosmetic and medical industries. It contains two important products: curcuminoids and volatile oils. Three types of curcuminoids exist: curcumin (the major compound), monodesmethoxycurcumin, and bisdes-methoxycurcumin. Curcuminoids can remedy various diseases such as peptic ulcers, gastric ulcers, dyspepsia, and metabolic and inflammatory conditions. However, monoculture cropping and the practice of rhizome propagation can lead to pathogen contamination, reducing the quality and quantity of curcuminoids. This study aimed to propagate in vitro C. longa plants and improve curcuminoid yield. Rhizomes were propagated using a micropropagation technique, and multiple shoot growth was induced on Murashige and Skoog media supplemented with 5 mgL^-1 kinetin and 1 mgL^-1 BA (8.00±0.58 shoots). Shoots grown on MS media with the addition of 5 µM phenylalanine, 5 µM malonic acid, and a growth retardant (ancymidol) exhibited the highest curcuminoid accumulation (2.31±0.061 µgg-1 dry weight). It can be concluded that the addition of phenylalanine and malonic acid can stimulate curcuminoid biosynthesis. Therefore, supplementing certain precursors, such as leguminous plants and green grasses, as planting material before cultivation can help increase curcuminoid levels.

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Author Biography

Oranual Hanmeng, Science Department, Faculty of Science and Technology, Thepsatri Rajabhat University, Lopburi, 15000

Science Department, Faculty of Science and Technology, Thepsatri Rajabhat University, Lopburi, 15000

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