EFFECTS OF CARBON SOURCES, PLANT GROWTH REGULATORS AND INOCULUM SIZE ON Citrus suhuiensis CELL SUSPENSION CULTURE GROWTH

NOOR ILLI  MOHAMAD PUAD; NUR ALIA  M. FATHIL; AZURA  AMID; AZLIN SUHAIDA  AZMI

doi:10.55230/mabjournal.v49i1.1658

Authors

NOOR ILLI MOHAMAD PUAD
illi@iium.edu.my
Bioprocess and Molecular Engineering Research Unit (BPMERU), Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10, 50728 Kuala Lumpur, Malaysia
NUR ALIA M. FATHIL Bioprocess and Molecular Engineering Research Unit (BPMERU), Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10, 50728 Kuala Lumpur, Malaysia
AZURA AMID International Institute for Halal Research and Training (INHART), International Islamic University Malaysia P.O. Box 10, 50728 Kuala Lumpur, Malaysia
AZLIN SUHAIDA AZMI Bioprocess and Molecular Engineering Research Unit (BPMERU), Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia (IIUM), P.O. Box 10, 50728 Kuala Lumpur, Malaysia

Keywords:

Citrus suhuiensis, cell suspension culture, carbon source, inoculum size, plant growth regulators (PGRs)

Abstract

Cell suspension culture of Citrus suhuiensis (C. suhuiensis) was established to determine the best growth condition based on the effects of carbon source, plant growth regulators (PGRs) and inoculum size. Friable callus from the cotyledon explant was used to initiate the cell suspension cultures. Murashige and Skoog (MS) medium supplemented with 50 g/L glucose achieved the highest cell dry weight (CDW) and µ_max of 23.2 g/L and 0.47 h-1 respectively compared to glucose at 30 g/L and other carbon sources. For PGRs effects, 0.5 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) gave the highest amount of CDW and µ_max; 11.91 g/L and 0.32 h-1. Furthermore, the inoculum size at 10% (v/v) gave the maximum CDW and µ_max of

15.45 g/L and 0.71 h-1 respectively compared to 20% (v/v) and 30% (v/v) of inoculum. The results achieved can be used for further statistical optimization of C. suhuiensis cell suspension culture growth parameters in which the interactions between those parameters can be further studied and understood.