PRODUCTION AND TOXICITY EVALUATION OF RHAMNOLIPIDS PRODUCED BY Pseudomonas STRAINS ON L6 AND HepG2 CELLS

Authors

  • ARULARASU MUTHALIAR TAMOTHRAN School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • VIGNESWARI SEVAKUMARAN School of Fundamental Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Institute Marine Biotechnology (IMB), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia
  • KESAVEN BHUBALAN School of Marine and Environmental Sciences, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Institute Marine Biotechnology (IMB), Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Malaysian Institute of Pharmaceuticals and Nutraceuticals (IPharm), NIBM, 11700 Gelugor, Pulau Pinang, Malaysia

Keywords:

Pseudomonas, biosurfactant, rhamnolipid, cytotoxicity, marine bacteria

Abstract

Biosurfactant rhamnolipid (RL) production using renewable resource is gaining attraction for commercial application. In this study, RL produced from three different strains of Pseudomonas using glycerol as a carbon source was used to evaluate toxicity towards rat skeletal muscle (L6) and liver cancer (HepG2) cells. In the present study, Pseudomonas aeruginosa PAO1 produced the highest concentration of RL (1.53 ± 0.28 g/L) and able to reduce the surface tension (ST) value of water the lowest (29.1 ± 0.5 mN/m). Toxicity evaluation using MTT assay indicated that RL produced does not have a cytotoxic effect towards both cell lines except where 50% inhibition concentration (IC50) was detected for HepG2 only at high concentration (100 µg/mL) for RL produced by P. aeruginosa PAO1. The RL produced by strains in this study is nontoxic with good ST reducing ability that has potential applications in food, cosmetics and pharmaceutical sector.

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Published

30-06-2019

How to Cite

TAMOTHRAN, A. M., SEVAKUMARAN, V. ., & BHUBALAN, K. (2019). PRODUCTION AND TOXICITY EVALUATION OF RHAMNOLIPIDS PRODUCED BY Pseudomonas STRAINS ON L6 AND HepG2 CELLS. Malaysian Applied Biology, 48(3), 149–156. Retrieved from https://jms.mabjournal.com/index.php/mab/article/view/1850

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Section

Research Articles