BIO-DEGRADATION OF POLYHYDROXYALKANOATES (PHA) FILMS IN SOIL AND LAKE ENVIRONMENT

Authors

  • VIGNESWARI SEVAKUMARAN School of Fundamental Science, Universiti Malaysia Terengganu, 21300 Kuala Nerus, Terengganu, Malaysia
  • NURUL SHUHADA RASHID School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
  • AMIRUL AL-ASHRAF ABDULLAH School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia; Malaysian Institute of Pharmaceuticals and Nutraceuticals, NIBM, 11700 Gelugor, Pulau Pinang, Malaysia

Keywords:

PHA, P(3HB-co-4HB), degradation

Abstract

The use of petroleum-based synthetic plastics has led to a deleterious solid waste management especially in the form of marine debris and presents a major growing global pollution problem. In response to these issues, the application of biobased and biodegradable polymers as an alternative to synthetic plastics has been proposed. Polyhydroxyalkanoates (PHA) is a biodegradable microbial polymer. In this study, the biodegradation of this PHA both in soil and lake environment was evaluated. The percentage of degradation of the PHAs with various monomers such as poly (3-hydroxybutrate) [P(3HB)] and its copolymers poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB] in soil and lake was carried out. Besides, the modifications of these biopolymers into salt-leached films were also tested. Based on the results obtained, P(3HB-co-4HB) films showed the highest rate of degradation for both lake and soil environment. Also the degradation of PHA, mainly caused by microbial activity, isolation and identification microorganisms capable of degrading PHA was also carried out. Based on the degradation index, Pseudomonas species and Acidovorax species were isolated.

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References

Altaee, N., El-Hiti, G.A., Fahdil, A., Sudesh, K. & Yousif, E. 2016. Biodegradation of different formulations of polyhydroxybutyrate films in soil. SpringerPlus, 5(1): 762.

Amirul, A.A., Ahmad, R.M. Yahya, Sudesh, K., Azizan, M.N.M. & Majid, M.I.A. 2011. Isolation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) producer from Malaysian environment using gamma-butyrolactone as carbon source. World Journal of Microbiology and Biotechnology, 25: 1199-1206.

Debbarma, P., Raghuwanshi, S., Singh, J., Suyal, D.C., Zaidi, M.G.H. & Goel, R. 2017. Comparative in situ biodegradation studies of polyhydroxybutyrate film composites. Biotechnology, 7(3): 178.

Faezah, A.N., Rahayu, A., Vigneswari, S., Majid, M.I.A. & Amirul, A.A. 2011. Regulating the molar fraction of 4-hydroxybutyrate in Poly(3- hydroxybutyrate-co-4-hydroxybutyrate) by biological fermentation and enzymatic degradation. World Journal of Microbiology and Biotechnology, 27(10): 2455-2459.

Handrick, R., Reinhardt, S., Focarete, M.L., Scandola, M., Adamus, G., Kowalczuk, M. & Jendrossek, D. 2001. A new type of thermoalkalophilic hydrolase of Paucimonas lemoignei with high specificity for amorphous polyesters of short chain-length hydroxyalkanoic acids. Journal of Biological Chemistry, 276(39): 36215-36224.

Martínez-Tobón, D.I., Gul, M., Elias, A.L. & Sauvageau, D. 2018. Polyhydroxybutyrate (PHB) biodegradation using bacterial strains with demonstrated and predicted PHB depolymerase activity. Applied Microbiology and Biotechnology, 102(18): 8049-8067.

Numata, K., Abe, H. & Iwata, T. 2009. Biodegradability of poly (hydroxyalkanoate) materials. Materials, 2(3): 1104-1126.

Ong, S.Y., Chee, J.Y. & Sudesh, K. 2017. Degradation of Polyhydroxyalkanoate (PHA): a Review. Journal of Siberian Federal University, 10(2): 211-225.

Salim, Y.S., Sharon, A., Vigneswari, S., Mohamad Ibrahim, M.N. & Amirul, A.A. 2012. Environmental degradation of microbial polyhydroxyalkanoates and oil palm-based composites. Applied Biochemistry and Biotechnology, 167(2): 314-326.

Sridewi, N., Bhubalan, K. & Sudesh, K. 2006. Degradation of commercially important polyhydroxyalkanoates in tropical mangrove ecosystem. Polymer Degradation anda Stability, 91: 2931-2940.

Sudesh, K. & Doi, Y. 2005. Polyhydroxyalkanoates. In: Bastioli, C. (Ed.), Handbook of Biodegradable Polymers. RAPRA Technology Ltd., United Kingdom. 219-256.

Vigneswari, S., Lee, T.S., Bhubalan, K. & Amirul, A.A. 2015. Extracellular polyhydroxyalkanoate depolymerase by Acidovorax sp. DP5. Enzyme Research, 2015.

Published

20-03-2019

How to Cite

SEVAKUMARAN, V., RASHID, N. S. ., & ABDULLAH, A. A.-A. (2019). BIO-DEGRADATION OF POLYHYDROXYALKANOATES (PHA) FILMS IN SOIL AND LAKE ENVIRONMENT. Malaysian Applied Biology, 48(1), 193–198. Retrieved from https://jms.mabjournal.com/index.php/mab/article/view/2316