CHARACTERIZATION AND MYCOTOXIN ANALYSIS OF Fusarium spp. FROM HIGHLAND AREAS IN MALAYSIA

NURHAZRIATI  MANSHOR; NURUL FARIZAH  AZUDDIN; MASRATUL HAWA  MOHD; NIK MOHD IZHAM  MOHAMED NOR; LATIFFAH  ZAKARIA

doi:10.55230/mabjournal.v51i3.2237

Authors

NURHAZRIATI MANSHOR chool of Biological Sciences, Universiti Sains Malaysia, 11800 USM Penang, Malaysia
NURUL FARIZAH AZUDDIN School of Biological Sciences, Universiti Sains Malaysia, 11800 USM Penang, Malaysia
MASRATUL HAWA MOHD School of Biological Sciences, Universiti Sains Malaysia, 11800 USM Penang, Malaysia
NIK MOHD IZHAM MOHAMED NOR School of Biological Sciences, Universiti Sains Malaysia, 11800 USM Penang, Malaysia
LATIFFAH ZAKARIA
Lfah@usm.my
School of Biological Sciences, Universiti Sains Malaysia, 11800 USM Penang, Malaysia

Keywords:

Fusarium graminearum species complex, Fusarium venenatum, Fusarium avenaceum, Fusarium kyushuense, highland areas, mycotoxin

Abstract

Fusarium isolates from highland areas in Malaysia were mostly recovered from two species of grasses, Elyhordeum montanense and Paspalum conjugatum. The isolates were grouped into four morphological groups. Based on TEF-1α sequences, morphotype 1 isolates were molecularly identified as F. graminearum species complex, morphotype 2 as F. venenatum, morphotype 3 as F. avenaceum and morphotype 4 as F. kyushuense. Restriction analysis of the Intergenic Spacer region showed high levels of genetic diversity of isolates in F. graminearum species complex and F. venenatum. For mycotoxin analysis, only F. avenaceum and F. kyushuense produced beauvericin and moniliformin (0.869 & 0.321 µg/kg, respectively). Zearalenone was produced by 32 isolates of F. graminearum species complex (0.002 – 0.437 µg/kg), two isolates of F. venenatum (0.006 - 0.014 µg/kg) and F. kyushuense (0.006 µg/kg). Only F. avenaceum isolate produced fumonisin B1 (0.001 µg/kg). The present study indicates the occurrence of Fusarium species commonly reported in highland areas in Malaysia where the weather is cooler and the temperature is lower than in the lowland areas. To our knowledge, this is the first report on the occurrence of phylogenetic species within F. graminearum species complex, F. venenatum, F. avenaceum and F. kyushuense in Malaysia.

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