• SUHANA SAMAT Faculty of Pharmacy, Universiti Teknologi MARA Selangor, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia
  • MUHAMMAD ASHRAF MOHD SALLEH Faculty of Pharmacy, Universiti Teknologi MARA Selangor, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor, Malaysia
  • ZAINAH ADAM Medical Technology Division, Malaysian Nuclear Agency, Bangi, 43000 Kajang, Selangor, Malaysia
  • WAN IRYANI WAN ISMAIL School of Fundamental Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia


3T3-L1 adipocytes, adipocyte size, pure honey, pineapple honey


Honey has potential in controlling obesity by reducing excess weight gain and other obesity parameters such as triglyceride levels. However, its effects on the cells that stores lipid (adipocytes) is still unclear. This study was performed to observe the effects of pineapple honey on the growth and lipid accumulation of adipocytes in vitro. Pineapple honey was standardised according to its total phenolic and flavonoid contents prior to treating on differentiated 3T3-L1 adipocytes. Proliferation of adipocytes was observed using 3-(4,5 dimethylthiazol-2-yl)-2,5-dipenyltetrazolium bromide (MTT) assay while lipid accumulation and droplet size were determined using oil red O staining and Feret’s diameter. Pineapple honey exhibited 0.0379 ± 0.001 mg/100 mL GAE of total phenolic content and 0.098 ± 0.001 mg catechin/kg of total flavonoid content. It significantly inhibited adipocytes’ proliferation starting from 6.25% of pineapple honey concentration. In addition, the honey also significantly reduced lipid droplet size by 33.78% to 70.36% and reduced lipid accumulation compared to the control. These findings suggest that pineapple honey may affect the storage of lipids in adipocytes. Future investigation involving the biomarkers of adipogenesis is required to confirm whether the reduction in lipid accumulation is attributed to the effect of honey on these pathways.


Download data is not yet available.


Metrics Loading ...


Ajibola, A.W., Chamunorwa, J.P. & Erlwanger, K.H. 2013. Comparative effect of cane syrup and natural honey on abdominal viscera of growing male and female rats. Indian Journal of Experimental Biology, 51: 303-312.

Almey, A.A.A., Khan, C.I.A.J., Syed, K.Z., Suleiman, M.R., Aisyah, M. & Rahim, K.K. 2010. Total phenolic content and primary antioxidant activity of methanolic and ethanolic extracts of aromatic plants’ leaves. International Food Research Journal, 17: 1077-1084.

Alvarez-Suarez, J., Tulipani, S., Romandini, S., Bertoli, E., Battino, M. & Fawcett, K.A. 2010. Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism, 3: 15-23.

Alvarez-Suarez, J., Giampieri, F. & Battino, M. 2013. Honey as a source of dietary antioxidants: Structures, bioavailability and evidence of protective effects against human chronic diseases. Current Medicinal Chemistry, 20: 1-18.

Bogdanov, S., Jurendic, T., Sieber, R., Gallmann, P., Jasmin, R.F. & Fawcett, K.A. 2012. Honey as nutrient and functional food. Journal of the American College of Nutrition, 40: 1-37.

Chepulis, L.M. 2007. The effects of honey compared with sucrose and a sugar-free diet on neutrophil phagocytosis and lymphocyte numbers after long-term feeding in rats. Journal of Complementary and Integrative Medicine, 4: 1-9.

Chepulis, L. & Starkey, N. 2008. The long-term effects of feeding honey compared to sucrose and a sugar-free diet on weight gain, lipid profiles, and DEXA measurements in rats. Journal of Food Science, 73: 1-7.

Hussein, S.Z., Yusoff, K.M., Makpol, S. & Yusof, Y.A.M. 2011. Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey. Molecules, 16: 6378-6395.

Ismail, I.S., Bebakar, W.M.W., Kamaruddin, N.A., Abdullah, N.H., Zin, F.M. & Taib, S.H.M. 2004. Clinical practice guideline on management of obesity. Ministry of Health Malaysia, 1-31.

Jia, Z., Tang, M. & Wu, J. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64: 555-559.

Mohd-Radzman, N.H., Ismail, W.I.W., Jaapar, S.S., Adam, Z. & Adam, A. 2013. Stevioside from Stevia rebaudiana Bertoni increases insulin sensitivity in 3T3-L1 adipocytes. EvidenceBased Complementary and Alternative Medicine, 1-11.

Moniruzzaman, M.I.K., Sulaiman, S.A. & Gan, S.H. 2013. Physicochemical and antioxidant properties of Malaysian honeys produced by Apis cerana, Apis dorsata and Apis mellifera. BMC Complementary and Alternative Medicine, 13: 1-12.

Mushtaq, R., Mushtaq, R. & Khan, Z.T. 2011. Effects of natural honey on lipid profile and body weight in normal weight and obese adults: a randomized clinical trial. Pakistan Journal Zoology, 43: 161-169.

Nemoseck, T.M., Carmody, E.G., Furchner-Evanson, A., Gleason, M., Li, A., Potter, H., Rezende, L.M., Lane, K.J. & Kern, M. 2011. Honey promotes lower weight gain, adiposity, and triglycerides than sucrose in rats. Nutrition Research, 31(1): 55-60.

Nwobodo, N.N. 2015. Toxicity and safety concerns in Orlistat therapy for obesity: a critical evaluation. Asian Journal of Biomedical and Pharmaceutical Sciences, 5(47): 01-04.

Power, C., Pereira, S.M.P., Law, C. & Ki, M. 2014. Obesity and risk factors for cardiovascular disease and type 2 diabetes: Investigating the role of physical activity and sedentary behaviour in mid-life in the 1958 British cohort. Atherosclerosis, 233(2): 363-369.

Rizzatti, V., Boschi, F., Pedrotti, M., Zoico, E., Sbarbati, A. & Zamboni, M. 2013. Lipid droplets characterization in adipocyte differentiated 3T3-L1 cells: size and optical density distribution. European Journal of Histochemistry, 57: 159-162.

Romero-Silva, S., Martinez, R.M.A., RomeroRomero, L.P., Rodriguez, O., Gerardo, G.C.S. & Morel, N. 2011. Effects of honey against the accumulation of adipose tissue and the increased blood pressure on carbohydrateinduced obesity in rat. Letters in Drug Design and Discovery, 8: 69-75.

Samat, S., Kanyan Enchang, F., Nor Hussein, F. & Wan Ismail, W.I. 2017. Four-week consumption of Malaysian honey reduces excess weight gain and improves obesity-related parameters in high fat diet induced obese rats. Evidence-Based Complementary and Alternative Medicine, 2017: 1-9.

Sharma, M., Dwivedi, P., Rawat, A.K.S.A. & Dwivedi, K. 2016. Nutrition nutraceuticals: a proactive approach for healthcare. Nutraceuticals, 79 116.

Song, Y., Park, H.J., Kang, S.N., Jang, S.H., Lee, S.J. & Ko, Y.G. 2013. Blueberry peel extracts inhibit adipogenesis in 3T3-L1 cells and reduce highfat diet-induced obesity, Public Library of Science One, 8: 1-12.

Teo, P.S., Nurul-Fadhilah, A., Aziz, M.E., Hills, A.P. & Foo, L.H. 2014. Lifestyle practices and obesity in Malaysian adolescents. International Journal of Environmental Research and Public Health, 11: 5828-5838.

Yaghoobi, A.W.N., Ghayour-Mobarhan, N., Parizadeh, M., Abasalti, S.M.R. & Yaghoobi, Z. 2008. Natural honey and cardiovascular risk factors; effects on blood glucose, cholesterol, triacylglycerole, CRP and body weight compared with sucrose. Science World Journal, 8: 463-469.

Wang, S., Moustaid-Moussa, N., Chen, L., Mo, H., Shastri, A., Su, R., Bapat, P., Kwun, I. & Shen, C.L. 2014. Novel insights of dietary polyphenols and obesity. Journal of Nutritional Biochemistry, 25(1): 1-18.



How to Cite