Optimizing In Vitro Surface Sterilization of Cyathea latebrosa Spore

Nurul Nadhirah; Haja Maideen; Zuraida Ab Rahman; Ayu Nazreena Othman

doi:10.55230/mabjournal.v51i5.2422

Authors

Nurul Nadhirah Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Haja Maideen
deen@ukm.edu.my
Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Zuraida Ab Rahman Biotechnology and Nanotechnology Research Centre, Malaysia Agricultural Research and Development Institute MARDI HQ, Persiaran MARDI-UPM, 43400 Serdang, Malaysia
Ayu Nazreena Othman Biotechnology and Nanotechnology Research Centre, Malaysia Agricultural Research and Development Institute MARDI HQ, Persiaran MARDI-UPM, 43400 Serdang, Malaysia

Keywords:

fern, spore, in vitro culture, surface sterilization

Abstract

Cyathea latebrosa is one of the lowland tree fern species found in Peninsular Malaysia. This fern species is highly demanded in ornamental landscaping. The in vitro cultures are an important tool for propagation which may contribute toward the reduction of over-exploitation. To overcome these problems, an effective spore surface disinfection protocol is crucial to allow the germination stage to be carried out. This studied had carried out three types of methods which are the packet method (PM), centrifuged method (CM), and soak method (SM) with difference percent of concentration (0, 0.1, 0.5, 1.0, 10 & 30) Mercury Chloride (HgCl₂) and Sodium hypochlorite (NaOCl). In contrast, the method and concentration of disinfection affect germination. Our results showed that the soak method in both types of disinfection is a significant difference due to statistical analysis (MANOVA) which gives a positive effect on the germination of the spore. This method is efficient for sterilizing which spore loss is kept to a minimum and has a higher rate of germination (HgCl₂-90% & NaOCl-80%). The optimum concentration of HgCl₂ was 0.1%, then followed by 0.5% and 1.0%, while for NaOCl was 30%, 20%, and 10%.

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Author Biography

Haja Maideen, Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

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