Anti-microbial Mechanism of Lonicera japonica and Andrographis paniculata Extract in Inhibiting Pseudomonas sp.

https://doi.org/10.55230/mabjournal.v52i3.2679

Authors

  • Wong Kok Kee Faculty of Health and Life Science, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia
  • Wong Rui Rui Faculty of Health and Life Science, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia https://orcid.org/0000-0002-0028-0159
  • Yuka Hara Faculty of Health and Life Science, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia https://orcid.org/0000-0002-5044-1942

Keywords:

biofilm, ethanol extract, membrane disruption, phenolic compound, swarming

Abstract

Antibiotic resistance reported in Pseudomonas sp. is associated with various opportunistic infections and is a concern in the public health system. The present study investigated the inhibitory effects of ethanol extracts of Lonicera japonica and Andrographis paniculata on Pseudomonas sp. The total phenolic compound (TPC) calculated in gallic acid equivalent (GAE) was 508.6±117.96 mg GAE/100 g dried weight (DW) in L. japonica while A. paniculata contained 129.0 ± 21.00 mg GAE/100 g DW. Both herbs significantly reduced the swarming zone diameters of Pseudomonas sp., even at a concentration as low as 5 mg/mL when compared to the controls. The anti-swarming effect of L. japonica and A. paniculata may be one of the mutual mechanisms underlying their anti-microbial properties. However, only the extract of L. japonica showed cell anti-adhesion activity, which can prevent biofilm formation. Andrographis paniculata extract on the other hand shows the highest activity in disrupting the cell membrane of Pseudomonas sp.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Abdelhady, M.I.S., Motaal, A.A. & Beerhues, L. 2011. Total phenolic content and antioxidant activity of standardized extracts from leaves and cell cultures of three callistemon species. American Journal of Plant Sciences, 2: 847-850. DOI: https://doi.org/10.4236/ajps.2011.26100

Bandara, M.B.K., Zhu, H., Sankaridurg, P.R. & Willcox, M.D.P. 2006. Salicylic acid reduces the production of several potential virulence factors of Pseudomonas aeruginosa associated with microbial keratitis. Investigative Ophthalmology & Visual Science, 47: 4453-4460. DOI: https://doi.org/10.1167/iovs.06-0288

Banerjee, M., Moulick, S., Bhattacharya, K.K., Parai, D., Chattopadhyay, S. & Mukherjee. S.K. 2017. Attenuation of Pseudomonas aeruginosa quorum sensing, virulence and biofilm formation by extracts of Andrographis paniculata. Microbial Pathogenesis, 113: 85-93. DOI: https://doi.org/10.1016/j.micpath.2017.10.023

Bouyahya, A., Dakka, N., Et-Touys, A., Abrini, J. & Bakri, Y. 2017. Medicinal plant products targeting quorum sensing for combating bacterial infections. Asian Pacific Journal of Tropical Medicine, 10: 729-743. DOI: https://doi.org/10.1016/j.apjtm.2017.07.021

Chaves, T.P., Clementino, E.L.C., Felismino, D.C., Rômulo, R.N., Vasconcellos, A.A., Coutinho, H.D.M. & Medeiros, A.C.D. 2014. Antibiotic resistance modulation by natural products obtained from Nasutitermes corniger (Motschulsky, 1855) and its nest. Saudi Journal of Biological Sciences. 22: 404-408. DOI: https://doi.org/10.1016/j.sjbs.2014.12.005

Chen, Q.Z., Lin, R.C., Wang, G.L. & Li, F.M. 2010. Studies on chemical constituents of the extract of Lonicera japonica. Journal of Chinese Medicinal Materials, 33(6): 920-922.

Clementi, E.A., Marks, L.R., Roche-Håkansson, H. & Håkansson, A.P. 2014. Monitoring changes in membrane polarity, membrane integrity, and intracellular ion concentrations in Streptococcus pneumoniae using fluorescent dyes. Journal of Visualized Experiments, 84: 1-8. DOI: https://doi.org/10.3791/51008-v

Fujii, A., Seki, M., Higashiguchi, M., Tachibana, I., Kumanogoh, A. & Tomono, K. 2014. Community-acquired, hospital-acquired, and healthcare-associated pneumonia caused by Pseudomonas aeruginosa. Respiratory Medicine Case Reports, 12: 30–33. DOI: https://doi.org/10.1016/j.rmcr.2014.03.002

Geetha, I. & Catherine, P.A.S. 2017. Antibacterial activity of Andrographis paniculata extracts. The Pharma Innovation Journal, 6: 1-4.

Gukui, C., Jiashen, Z., Yili, Z., Weiping, H., Juan, P., Meng, L., Yani, Z., Tietao, W., Lin, Z., Liang, Z. & Haihua, L. 2021. Structural basis for diguanylate cyclase activation by its binding partner in Pseudomonas aeruginosa. eLife, 10: 1-21.

Jarukamjorn, K. & Nemoto, N. 2008. Pharmacological aspects of Andrographis paniculata on health and its major diterpenoid constituent andrographolide. Journal of Health Sciences, 54: 370-381. DOI: https://doi.org/10.1248/jhs.54.370

Jayanthi, B., Emenike, C.U., Agamuthu, P., Simarani, K., Mohamad, S. & Fauziah, S.H. 2016. Selected microbial diversity of contaminated landfill soil of Peninsular Malaysia and the behavior towards heavy metal exposure. Catena, 147: 25-31. DOI: https://doi.org/10.1016/j.catena.2016.06.033

Kaur, C. & Kapoor, H.C. 2002. Anti-oxidant activity and total phenolic content of some Asian vegetables. The International Journal of Food Science & Technology, 37: 153-161. DOI: https://doi.org/10.1046/j.1365-2621.2002.00552.x

Kok-Kee, W., Brid, Q., Ainon, H. & Salmijah, S. 2015. Phenol biodegradation and metal removal by a mixed bacterial consortium. Bioremediation Journal, 19(2): 104-112. DOI: https://doi.org/10.1080/10889868.2014.995368

Kwil, I., Kaźmierczak, D. & Różalski, A. 2013. Swarming growth and resistance of Proteus penneri and Proteus vulgaris strains to normal human serum. Advances in Clinical and Experimental Medicine, 22: 165-75.

Moore, L.S.P., Cunningham, J., & Donaldson, H. 2016. A clinical approach to managing Pseudomonas aeruginosa infection. British Journal of Hospital Medicine, 77(4): 50-54. DOI: https://doi.org/10.12968/hmed.2016.77.4.C50

Myszk, K., Schmid, M., Bialas, W., Olkowicz, M., Leja, K. & Czaczyk, K. 2015. Role of gallic and p -coumaric acids in the AHL-dependent expression of flgA gene and in the process of biofilm formation in food-associated Pseudomonas fluorescens KM120. Journal of the Science of Food and Agriculture, 96: 4037-4047. DOI: https://doi.org/10.1002/jsfa.7599

Praveen, N., Naik, P.M. & Nayeem, A. 2014. Polyphenol composition and antioxidant activity of Andrographis paniculata L. Nees. Mapana Journal of Sciences, 13: 33-46. DOI: https://doi.org/10.12723/mjs.31.4

Rol, C.K., Joon, T.Y., Yoke, C M., Shun, T.J., Abbasiliasi, S., Kee, W.K. & Hock, O.G. 2022. Preliminary assessment of Polytrichum commune extract as an antimicrobial soap ingredient. Journal of Experimental Biology and Agricultural Sciences, 10(4): 894–901. DOI: https://doi.org/10.18006/2022.10(4).894.901

Rufino, R.D., Luna, J.M., Sarubbo, L.A., Rodrigues, L.R.M., Teixeira, J.A.C. & Campos-Takaki, G.M. 2011. Antimicrobial and anti-adhesive potential of a biosurfactant Rufisan produced by Candida lipolytica UCP 988. Colloids and Surfaces B: Biointerfaces, 84(1): 1-5, DOI: https://doi.org/10.1016/j.colsurfb.2010.10.045

Shang, X., Pan, H., Li, M., Miao, X. & Ding, H. 2011. Lonicera japonica Thunb.: ethnopharmacology, phytochemistry and pharmacology of an important traditional Chinese medicine. Journal of Ethnopharmacology, 138: 1–21. DOI: https://doi.org/10.1016/j.jep.2011.08.016

Subramanian, K., Selvakkumar, C., Vinaykumar, K.S., Goswami, N., Meenakshisundaram, S., Balakrishnan, A. & Lakshmi, B.S. 2009. Tackling multiple antibiotic resistance in enteropathogenic Escherichia coli (EPEC) clinical isolates: a diarylheptanoid from Alpinia officinarum shows promising antibacterial and immunomodulatory activity against EPEC and its lipopolysaccharide-induced inflammation. International Journal of Antimicrobial Agents, 33: 244–250. DOI: https://doi.org/10.1016/j.ijantimicag.2008.08.032

Tong F.S., Jayanthi, B., Chang, S.K. & Wong, K.K. 2022. Characterisation and toxicity evaluation of a biosurfactant produced from Pseudomonas sp. Current topics in toxicology, 18: 141-145.

Truchado, P., Tomás-Barberán, F., Larrosa, M. & Allende, A. 2012. Food phytochemicals act as Quorum Sensing inhibitors reducing production and/or degrading autoinducers of Yersinia enterocolitica and Erwinia carotovora. Food Control, 24: 78-85. DOI: https://doi.org/10.1016/j.foodcont.2011.09.006

Ugurlu, A., Yagci, A.K., Ulusay, S., Aksu, B. & Bosgelmez-Tinaz, G. 2016. Phenolic compounds affect production of pyocyanin, swarming motility and biofilm formation of Pseudomonas aeruginosa. Asian Pacific Journal of Tropical Biomedicine, 6: 698-701. DOI: https://doi.org/10.1016/j.apjtb.2016.06.008

Vasavi, H.S., Arun, A.B. & Rekhab, P. 2014. Anti-quorum sensing activity of Psidium guajava L. flavonoids against Chromobacterium violaceum and Pseudomonas aeruginosa PAO1. Medical Microbiology and Immunology, 58(5): 286-293. DOI: https://doi.org/10.1111/1348-0421.12150

Wang, Y., Yu, J., Xiao, Y., Cheng, L. & Guan, X.Z. 2000. Studies on the elimination of resistance plasmid from P. aeruginosa in vitro and vivo with Lonicera japonica. Journal of Norman Bethune University of Medical Science, 26: 139–141.

Zaid, O.I., Abd Majid, R., Sabariah, M.N., Hasidah, M.S., Al-Zihiry, K., Yam, M.F. & Basir, R. 2015. Andrographolide effect on both Plasmodium falciparum infected and non infected RBCs membranes, Asian Pacific Journal of Tropical Medicine, 8(7): 507-512. DOI: https://doi.org/10.1016/j.apjtm.2015.06.007

Published

30-09-2023

How to Cite

Kee, W. K., Rui, W. R., & Hara, Y. (2023). Anti-microbial Mechanism of Lonicera japonica and Andrographis paniculata Extract in Inhibiting Pseudomonas sp. Malaysian Applied Biology, 52(3), 29–35. https://doi.org/10.55230/mabjournal.v52i3.2679

Issue

Section

Research Articles

Funding data

Most read articles by the same author(s)