Antioxidant Properties of Leaves Extracts of Acanthaceae Species


  • Anisa Saeed Al-Hakimi Biology Department, Faculty of Applied Science, Taiz University, Taiz, Yemen
  • Seham Saeed Faculty of Pharmacy, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
  • Naser Omar Department of Botany, Faculty of Arts and Sciences, University of Benghazi- Alabear Branch, Libya
  • Abdul Latiff Department of Biological Science and Biotechnology, Universiti Kebangsaan Malaysia, 43600 Bangi,Selangor, Malaysia


Acanthaceae, antioxidant properties, phenolic content, radical-scavenging effect, reducing power


The objective of this study is to determine the antioxidant properties of leaves extracts of 24 Acanthaceae species: Asystasia guttata, Ballochia amoena, Barleria aculeata, B. orbicularis, B. parviflora, B. prionitis, B. ventricosa, Blepharis cayaniense, B. maderaspatensis, Crossandra johanninae, Dicliptera effusa, D. paniculata, D. verticillata, Ecbolium gymnostachyum, Hypoestes pubescens, H. triflora, Justicia caerulea, J. heterocarpa, J. odora, Megalochalmyas violacea, Phaulopsis imbricata, Trichocalyx orbicular, Ruellia grandiflora and R. paulayana. The radical scavenging activities of 2,2 diphenyl-1-picrylhydrazyl, ferric-reducing antioxidant power, and total phenolic compounds of species were determined using the Folin-Ciocalteau reagent. Blepharis cayaniense has the highest phenolic compounds (995.5 mg of gallic acid per g of sample) while Hypoestes pubescens, and Ruellia paulayana (10 mg/mL gallic acid equivalent) have the lowest concentration of phenolic acid but less radical scavenging activities (DPPH) and reducing ferric power. Only Trichocalyx orbiculatus has the highest concentration in phenolic content, radical-scavenging activities, and reducing power. Interestingly, the antioxidant activities of different Acanthaceae species exhibit unique medicinal properties.


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Abubacker, M.N. & Kamala, D.P. 2017. Lepidagathis cristata willed. (Acanthaceae): A review of its phytochemical and pharmacology activities. Medical Research Archive, 5(5): 1-8.

Athar, A., Kosmulalage, S. & Kalhari, R. S. 2009. Chemical constituents of Barleria prionitis and their enzyme inhibitory and free radical scavenging activities. Phytochemistry Letters, 2(1): 37-40.

Babu, B.H., Shylesh, B.S. & Padikkala, J. 2002. Tumour reducing and anticarcinogenic activity of Acanthus ilicifolius in mice. Journal of Ethnopharmacology, 79: 27-33.

Bader, A., Martini, F., Schinella, G.R., Rios, J.L. & Prieto, J.M. 2015. Modulation of cox-1, 5-, 12- and 15-lox by popular herbal remedies used in southern Italy against psoriasis and other skin diseases. Phytotherapy Research, 29: 108-113.

Bency, A., Lohidas, J. & Murugan, M. 2018. Phytochemical studies and antibacterial activity of Barleria acuminata Nees., (Acanthaceae). Journal of Pharmacognosy and Phytochemistry, 7(4): 1909-1911.

Beyi, M.W. 2018. Ethnobotanical investigation of traditional medicinal plants in Dugda District, Oromia Regio. Journal of Medicinal Plants Studies, 2(1): 1007.

Bhatt, A., Naidoo, Y. & Nicholas, A. 2010. The foliar trichomes of Hypoestes aristata (Vahl) Sol. ex Roem. & Schult var aristata (Acanthaceae) a widespread medicinal plant species in tropical sub-Saharan Africa: with comments on its possible phylogenetic significance. Biology Research, 43: 403-409.

Bremner, P., Rivera, D., Calzado, M.A., Obon, C., Inocencio, C., Beckwith, C., Fiebich, B.L., Munoz, E. & Heinrich, M. 2009. Assessing medicinal plants from South-Eastern Spain for potential anti-inflammatory effects targeting nuclear factor-Kappa B and other pro-inflammatory mediators. Journal of Ethnopharmacology, 124: 295-305.

Dhanalakshmi, M. & Thangadurai, S.A. 2021. Antioxidant and Anticancer Activities of Whole Plant Extracts of Lepidagathis pungens Nees: In vitro: Evaluation. Pharmacognosy Magazine, 17(1): S63-S67.

Farombi, E.O. & Fakoya, A. 2005. Free radical scavenging and antigenotoxic activities of natural phenolic compounds in dried flowers of Hibiscus sabdariffa L. Molecular Nutrition & Food Research, 49: 1120-1128.

Finkel, T. & Holbrook, N.J. 2000. Oxidants, oxidative stress and the biology of ageing. Nature, 408(6809): 239-247.

Giorgi, M., Capocasa, F., Scalzo, J., Murri, G., Battino, M. & Mezzetti, B. 2005. The rootstock effects on plant adaptability, production, fruit quality, and nutrition in the peach (cv. 'Suncrest'). Scientia Horticulturae,107: 36-42.

Govindasamy, C. & Arulpriya, M. 2013. Antimicrobial activity of Acanthus ilicifolius: Skin infection pathogens. Asian Pacific Journal of Tropical Disease, 3: 180-183.

Hee, S.P., So, R.K., Ju, O.K. & Yong, C.L. 2010. The roles of phytochemicals in bronchial asthma. Molecules, 15: 6810-6834.

Huang, S.S., Chiu, C.S., Lin, T.H., Lee, M.M., Lee, C.Y., Chang, S.J., Hou, W.C., Huang, G.J. & Deng, J.S. 2013. Antioxidant and anti-inflammatory activities of aqueous extract of Centipeda minima. Journal of Ethnopharmacology, 147(2): 395-405.

Ibrahim, K., Sohail, A. J., Zabta, K.S., Muhammad, A., Yasmin, K. & Taweer, Kumar. 2017. Ethnobotany and medicinal uses of folklore medicinal plants belonging to family acanthaceae: An updated review. MOJ Biology and Medicine, 1(2): 34- 38.

Jara, C., Leyton, M., Osorio, M., Silva, V., Fleming, F., Paz, M., Madrid, A. & Mellado, M. 2017. Antioxidant, phenolic and antifungal profiles of Acanthus mollis (Acanthaceae). Natural Product Research, 31(19): 2325-2328.

Khlifi, D., Sghaier, R.M., Amouri, S., Laouini, D., Hamdi, M. & Bouajila, J. 2013. Composition and anti-oxidant, anti-cancer and anti-inflammatory activities of Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L. Food and Chemical Toxicology, 55: 202-208.

Madhu, V., Chinnaiah, B. & Swamy, T.N. 2010. Traditional herbal remedies to cure asthma in Adilabad District. International Journal of Pharmacy and Life Sciences, 1(4): 217-221.

Mani Senthil Kumar, K.T., Gorain, B., Roy, D.K., Samanta, S.K., Pal, M., Biswas, P., Roy, A., Adhikara, D. & Karmakar, S. 2008. Anti-inflammatory activity of Acanthus ilicifolius. Journal of Ethnopharmacology, 120: 7-12.

Manjula, M.S. & Anjalai, E. 2019. Evaluation of minerals and phytochemicals present in aerial parts of Barleria buxifolia L. (Acanthaceae). The Pharma Innovation Journal, 8(4): 309-311.

Manjula, M.S. & Saravana, G.A. 2018a. Nephroprotective activity of Barleria longiflora L. (Acanthaceae) against gentamicin induced nephrotoxicity in male albino wistar rats. Journal of Pharmacognosy and Phytochemistry, 7(2): 2835-2837.

Manjula, M.S. & Saravana, G.A. 2018b. In In-vitro antioxidant and anti-inflammatory potential of ethanol extracts (root and aerial parts) of Barleria noctiflora. Annals of Plant Sciences, 7(2):1997-2001.

Manjula, M.S. & Saravana, G.A. 2018c. In-vitro anti-diabetic activity of root and aerial parts of Barleria noctiflora L. f. (Acanthaceae) The Pharma Innovation Journal, 7(4): 1073-1075.

McDonald, S., Prenzler, P.D., Autolovich, M. & Robards, K. 2001. Phenolic content and antioxidant activity of olive oil extracts. Food Chemistry, 73: 73-84.

Miller, A.G. 2004. Ethnoflora of the Soqotra Archipelago. Royal Botanic Garden Kew. pp. 400-414.

Neena, A., Mahesh, Mohanan, P. & Tbomas, B. 2019. Phytochemical and antimicrobial evaluations of some selected plants in family Acanthaceae. Journal of Pharmaceutical Advance Research, 2(2): 479-483.

Phumthum, M. & Sadgrove, N.J. 2020. High-value plant species used for the treatment of "fever" by the Karen hill tribe people. Antibiotics, 9(5): 220.

Ponnusamy, S. & Balakrishnan, S. 2023. Genus Lepidagathis (Acanthaceae): Review of its ethnobotany, phytochemistry and pharmacological potential. International Journal of Pharmacy and Pharmaceutical Sciences, 15(5): 1-7.

Renjini, H., Sumathi, P., Sangeeth, T. & Binu, T. 2017. Qualitative phytochemical analysis of Barleria pratensis Sant. (Acanthaceae). International Journal of Research in Ayurveda and Pharma, 8(1): 97-100.

Scalzo, J., Politi, A., Pellegrini, N., Mezzetti, B. &Battino, M. 2005. Plant genotype affects total antioxidant capacity and phenolic contents in fruit. Nature, 21: 207-213.

Siriwatanametanon, N., Fiebich, B.L., Efferth, T., Prieto, J.M. & Heinrich, M. 2010. Traditionally used Thai medicinal plants: in vitro anti-inflammatory, anticancer and antioxidant activities. Journal of Ethnopharmacology, 130(2): 196-207.

Thaipong, K., Crosby, K., Cisneros-zevallos, L., Boonprakob, U. & Byrne, D.H. 2006. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. Journal of Food Composition and Analysis, 19: 669-675.

Thirunavukkarasu, P., Amanathan, T.& Ramkumar, L. 2011. Hemolytic and antimicrobial effect in the leaves of Acanthus ilicifolius. Journal of Pharmacology and Toxicology, 6: 196-200.

Wakuma, W., Solomon, A. & Wubayehu, K. 2021. Evaluation of antidiabetic and antioxidant activity of leaf extract and solvent fractions of Hypoestes forskaolii (Val) (Acanthaceae) in Mice. Journal of Experimental Pharmacology, 13: 859-872

Wood, J.R.I. 1997. A Hand Book of the Yemen Flora. Royal Botanic Gardens Kew.

Yahaufai, J., Siripong, P. and Limpanasithkul, W. 2010. Immunomodulatory Effect of Acanthus ebracteatus Val. Aqueus Extract on Macrophage Function. Thai Cancer Journal, 30: 55-67.

Yemane, B., Andebrhan, M. & Surender, R.K. 2017. Traditional medicinal plants used by Tigrigna ethnic group in central region of Eritrea. IOSR Journal of Pharmacy and Biological Sciences, 12(3): 40-46.


30-09-2023 — Updated on 08-10-2023


How to Cite

Al-Hakimi, A. S. ., Saeed, S., Omar, N., & Latiff, A. . (2023). Antioxidant Properties of Leaves Extracts of Acanthaceae Species. Malaysian Applied Biology, 52(3), 97–103. (Original work published September 30, 2023)



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