Keberkesanan Ekstrak Akua Kaliks Hibiscus sabdariffa Sebagai Agen Anti-Obesiti dan Peningkat parameter Kesuburan Tikus Jantan Aruhan Obesiti
Effectiveness of Hibiscus sabdariffa Calyx Aqueous Extract as an Anti-Obesity Agent and Enhancer of Fertility Parameters in Obese-Induced Male Rats
Keywords:
Hibiscus sabdariffa, indeks Lee, kesuburan, obesitiAbstract
Obesiti meningkatkan risiko gangguan proses spermatogenesis yang boleh mengakibatkan penurunan kesuburan lelaki. Hibiscus sabdariffa merupakan tumbuhan yang mampu mengurangkan jumlah lipid dalam darah haiwan. Penggunaan ekstrak akua kaliks H. sabdariffa sebagai peningkat parameter kesuburan lelaki masih kurang jelas. Oleh itu, kajian ini menggunakan tikus jantan aruhan obesiti sebagai model haiwan bagi mengenal pasti kesan ekstrak akua kaliks H. sabdariffa dalam memulihkan masalah kesuburan jantan di samping bertindak sebagai agen anti-obesiti. Sebanyak 36 ekor tikus jantan Sprague Dawley dibahagikan kepada dua kumpulan utama iaitu kumpulan kawalan dan kumpulan rawatan yang setiapnya terdiri daripada enam ekor tikus. Kumpulan kawalan terdiri daripada kumpulan normal, negatif (tikus teraruh obesiti tanpa rawatan) dan positif (tikus teraruh obesiti yang diberi 10 mg/kg berat tubuh orlistat). Kumpulan rawatan ekstrak akua kaliks H. sabdariffa pula dibahagi kepada tiga iaitu Hs100 (100 mg/kg berat tubuh), Hs200 (200 mg/kg berat tubuh) dan Hs300 (300 mg/kg berat tubuh) selama 14 hari tempoh rawatan. Semua tikus kajian kecuali kumpulan normal diaruh obesiti dengan diberi diet tinggi lemak (HFD) sehingga mencapai nilai indeks Lee melebihi 310. Tikus kumpulan rawatan Hs100, Hs200 dan Hs300 yang diberi ekstrak akua kaliks H. sabdariffa mengikut dos masing-masing menunjukkan penurunan berat badan yang signifikan (p<0.05) berbanding kumpulan negatif. Analisis kuantiti sperma dalam kumpulan rawatan Hs100 menunjukkan peningkatan secara signifikan (p<0.05) berbanding kumpulan normal dan negatif. Analisis histologi testis juga menunjukkan ekstrak akua kaliks H. sabdariffa pada kumpulan rawatan membantu memperbaiki struktur tubul seminiferus dengan kepadatan spermatozoa yang tinggi pada lumen serta peningkatan berat testis dan diameter tubul seminiferus berbanding kumpulan kawalan normal dan kawalan negatif. Kesimpulannya, rawatan ekstrak akua kaliks H. sabdariffa iaitu Hs100 merupakan dos optimum yang mampu meningkatkan parameter kesuburan berbanding kumpulan rawatan normal dan kawalan negatif, serta mempunyai kesan anti-obesiti pada tikus jantan teraruh obesiti.
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