Enhanced Cell Harvesting for Lipid Production by Lipomyces maratuensis InaCC Y720 Using Flocculation Induction
Keywords:
biomass, flocculation, lipids, Lipomyces maratuensis, SEMAbstract
Lipomyces maratuensis InaCC Y720 is a yeast from Indonesia that is known to be able to produce lipids on a production medium that has been optimized in the previous study. One of the efforts to increase the harvesting of yeast cells needed on a production scale larger than the laboratory scale can be done by flocculation induction. This study showed that flocculation induction significantly increased the cell harvest of Lipomyces maratuensis InaCC Y720, with the biomass obtained increasing up to 2.7 times compared to the method without flocculation induction. The combination of pH 9 adjustment and the addition of Ca2+ ions proved to be the optimal conditions to induce flocculation, resulting in cell aggregations that appeared adherent based on scanning electron microscopy (SEM). These findings suggest that the flocculation induction not only improves cell harvesting efficiency but also has the potential to be applied in industrial-scale lipid production as a more effective harvesting method. Although the main composition of the fatty acids produced remained consistent, there were differences in the percentage of each fatty acid between harvesting methods with and without flocculation induction. However, to maximize the utilization of lipids from flocculated cells, further research is needed to develop more efficient extraction methods. Thus, this study provides a solid foundation for further exploration in the optimization of lipid production using oleaginous yeast.
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Audinah, L. 2022. Optimasi Medium untuk Produksi Lipid oleh Lipomyces maratuensis InaCC Y720 menggunakan Desain Eksperimen Statistikal (M.Sc.). Institut Teknologi Bandung.
Bligh, E.G. & Dyer, W.J. 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37(8): 911-917.
Jin, Y.L., Ritcey, L.L., Speers, R.A. & Dolphin, P.J. 2001. Effect of cell surface hydrophobicity, charge, and zymolectin density on the flocculation of Saccharomyces cerevisiae. Journal of the American Society of Brewing Chemists, 59(1): 1-9.
Kedong, M., Minato, W., Kenji, S. & Yoshihito, S. 2010. Flocculation phenomenon of a mutant flocculent Saccharomyces cerevisiae strain: Effects of metal ions, sugars, temperature, pH, protein-denaturants and enzyme treatments. African Journal of Biotechnology, 9(7): 1037-1045.
Li, Q., Du, W. & Liu, D. 2008. Perspectives of microbial oils for biodiesel production. Applied Microbiology and Biotechnology, 80(5): 749-756.
Louhasakul, Y., Cheirsilp, B., Maneerat, S. & Prasertsan, P. 2018. Potential use of flocculating oleaginous yeasts for bioconversion of industrial wastes into biodiesel feedstocks. Renewable Energy, 136: 1311-1319.
Miki, B.L., Poon, N.H., James, A.P. & Seligy, V.L. 1982. Possible mechanism for flocculation interactions governed by gene FLO1 in Saccharomyces cerevisiae. Journal of Bacteriology, 150(2): 878-889.
Pienasthika, M., Brahmanti, A.A., Purwatiningrum, I. & Wardani, A.K. 2020. A study of the effect of calcium chloride (CaCl2) and pH on the flocculation ability of Saccharomyces cerevisiae (NCYC 1195). In: IOP Conference Series: Earth and Environmental Science. IOP Publishing, Bristol. pp. 012082.
Sander, K. & Murthy, G.S. 2010. Life cycle analysis of algae biodiesel. The International Journal of Life Cycle Assessment, 15(7): 704-714.
Sitepu, I.R., Garay, A.L., Cajka, T., Fiehn, O. & Boundy-Mills, K.L. 2019. Laboratory screening protocol to identify novel oleaginous yeasts. In: Methods in Molecular Biology. A. Sigurdsson (Ed.). Springer, New York. pp. 33-50.
Sitepu, I.R., Garay, L.A., Sestric, R., Levin, D., Block, D.E., German, J.B. & Boundy-Mills, K.L. 2014. Oleaginous yeasts for biodiesel: current and future trends in biology and production. Biotechnology Advances, 32(7): 1336-1360.
Soares, E.V. 2011. Flocculation in Saccharomyces cerevisiae: A review. Journal of Applied Microbiology, 110(1): 1-18.
Speers, R.A., Wan, Y.Q., Jin, Y.L. & Stewart, R.J. 2006. Effects of fermentation parameters and cell wall properties on yeast flocculation. Journal of the Institute of Brewing, 112(3): 246-254.
Stewart, G.G. 2017. Harvesting and cropping yeast: Flocculation and centrifugation. In: Brewing and Distilling Yeasts. G.G. Stewart (Ed.). Springer International Publishing, Cham. pp. 259-308.
Stewart, G.G., Hill, A.E. & Russell, I. 2013. 125th Anniversary Review: Developments in brewing and distilling yeast strains. Journal of the Institute of Brewing, 119(4): 202-220.
Stratford, M. 1989. Yeast flocculation: calcium specificity. Yeast, 5(6): 487-496.
Stratford, M. 1994. Genetic aspects of yeast flocculation: in role of FLO genes in the flocculation of. Colloids and Surfaces B: Biointerfaces, 2(1-3): 151-158.
Verstrepen, K.J. & Klis, F.M. 2006. Flocculation, adhesion and biofilm formation in yeasts. Molecular Microbiology, 60(1): 5-15.
Wilcocks, K.L. & Smart, K.A. 1995. The importance of surface charge and hydrophobicity for the flocculation of chain-forming brewing yeast strains and resistance of these parameters to acid washing. FEMS Microbiology Letters, 134(2-3): 293-297.
Yamazaki, A., Kanti, A. & Kawasaki, H. 2017. Three novel lipomycetaceous yeasts, Lipomyces maratuensis sp. nov., Lipomyces tropicalis sp. nov., and Lipomyces kalimantanensis f.a., sp. nov. isolated from soil from the Maratua and Kalimantan Islands, Indonesia. Mycoscience, 58(6): 413-423.
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