The Evaluation of Blast Resistance and Submergence Tolerance of New Breeding Rice (Oryza sativa L.) Lines Developed Through 4-Way Marker-Assisted Breeding
Keywords:
Blast resistance, fried keropok keping, Pi genes, submergence tolerance, Sub1 QTLsAbstract
This study aimed to create new rice lines with a strong resistance to blast disease and a high tolerance to submergence. This was achieved by introducing Pi and Sub1 QTLs into the popular local rice variety, Pulau Batu using a 4-way marker-assisted breeding technique. The progenies were evaluated both phenotypically and genotypically to identify those that have favorable traits. The 4-way-F3 rice breeding lines that showed exceptional performance were then assessed in both greenhouse and rice field nurseries from April to July 2023, corresponding to the dry season. The blast fungus, Magnaporthe oryzae (MoK19-28) isolated from a local rice field in West Sumatra was utilized as a fungal inoculum to assess the resistance level of established breeding lines against blast disease. Phenotypic blast resistance test was conducted according to the SES-blast-test standard. Consequently, a submergence tolerance test was carried out to assess the tolerance level of breeding lines to submergence over 14 days of vegetative development, following the submergence tolerance test standard. The results indicated that 11 breeding lines exhibited exceptional performance when exposed to blast disease and submergence stress. Blast resistance test showed that 60% of the breeding lines were categorized as resistant, 27% as moderately resistant, and 13% as susceptible. The submergence test indicated that 7% of the breeding lines were categorized as tolerant, 42% as moderately tolerant, 28% as moderately susceptible, and 23% as highly susceptible. Plants with a high survival rate (>70%) tend to have a low elongation percentage rate (<30%) and low changes in chlorophyll content (<30%). In the natural nursery, they exhibited superior performance in comparison to their parental lines, namely Pulau Batu, Inpari 48 Blas, and IR64-Sub1. This study proposed that the selected breeding lines combined Pi and Sub1A QTLs, which enhance phenotypic traits related to blast disease and submergence stress.
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Akos, I.S., Yusop, M.R., Ismail, M.R., Ramlee, S.I., Noraziyah, A.A.S., Ramli, A.B., Haliru, B.S., Ismai'la, M. & Chukwu, S.C. 2019. A review on gene pyramiding of agronomic, biotic and abiotic traits in rice variety development. International Journal of Applied Biology, 3(2): 65-84.
Ashkani, S., Yusop, M.R., Shabanimofrad, M., Harun, A.R., Sahebi, M. & Latif, M.A. 2015. Genetic analysis of resistance to rice blast: A study on the inheritance of resistance to the blast disease pathogen in an F3 population of rice. Journal of Phytopathology, 163(4): 300-309. DOI: https://doi.org/10.1111/jph.12323
Asibi, A.E., Chai, Q. & Coulter, J.A. 2019. Rice blast: A disease with implications for global food security. Agronomy, 9(8): 451-460. DOI: https://doi.org/10.3390/agronomy9080451
Bai, J., Pennill, L.A., Ning J., Lee, S.W. & Ramalingam, J. 2002. Diversity in nucleotide binding site-leucine-rich repeat genes in cereals. Genome Research, 12: 1871-1884. DOI: https://doi.org/10.1101/gr.454902
Boller, T. & He, S.Y. 2009. Innate immunity in plants: an arms race between pattern recognition receptors in plants and effectors in microbial pathogens. Science, 324(5928): 742-744. DOI: https://doi.org/10.1126/science.1171647
Bruce, A.K.K., Donkoh, S.A. & Ayamga, M. 2014. Improved rice variety adoption and its effects on farmers' output in Ghana. Journal of Development and Agricultural Economics, 6(6): 242-248. DOI: https://doi.org/10.5897/JDAE2013.0544
Dangl, J.L. & Jones, J.D. 2001. Plant pathogens and integrated defence responses to infection. Nature, 411(6839): 826-833. DOI: https://doi.org/10.1038/35081161
Dangl, J.L., Horvath, D.M. & Staskawicz, B.J. 2013. Pivoting the plant immune system from dissection to deployment. Science, 341(6147): 746-751. DOI: https://doi.org/10.1126/science.1236011
Daudi, A., Cheng, Z., O'Brien, J.A., Mammarella, N., Khan, S., Ausubel, F.M. & Bolwell, G.P. 2012. The apoplastic oxidative burst peroxidase in Arabidopsis is a major component of pattern-triggered immunity. The Plant Cell, 24(1): 275-287. DOI: https://doi.org/10.1105/tpc.111.093039
Filippi, M.C. & Prahbu, A.S. 2001. Phenotypic virulence analysis of Pyricularia grisea isolates from Brazilian upland rice cultivars. Pesqui Agropecu Bras, 36: 27-35. DOI: https://doi.org/10.1590/S0100-204X2001000100004
Fukuoka, S. & Okuno, K. 2019. Strategies for breeding durable resistance to rice blast using Pi21. Crop Breeding, Genetics, and Genomics, 1(2). DOI: https://doi.org/10.1270/jsbbr.19J15
Gramene Markers Database. URL https://archive.gramene.org/markers/ (accessed 04.14.20)
Hammond-Kosack, K.E. & Jones, J.D. 1997. Plant disease resistance genes. Annual Review of Plant Biology, 48(1): 575-607. DOI: https://doi.org/10.1146/annurev.arplant.48.1.575
Harborne, J.B. 1979. Phytochemical methods: A guide to modern techniques of plant analysis. Chapman and Hall Ltd, London.
Hartman, S., Sasidharan, R. & Voesenek, L.A. 2021. The role of ethylene in metabolic acclimations to low oxygen. New Phytologist, 229(1): 64-70. DOI: https://doi.org/10.1111/nph.16378
Hattori, Y., Nagai, K. & Ashikari, M. 2011. Rice growth adapting to deep water. Current Opinion in Plant Biology 14(1): 100-105. DOI: https://doi.org/10.1016/j.pbi.2010.09.008
IBM Corp. 2019. IBM SPSS Statistics for Windows, Version 26.0. IBM Corp., New York.
IRRI. 1996. International Network for Genetic Evaluation of Rice (Standard Evaluation System for Rice/SES). Los Banos (Philippines): International Rice Research Institute. http://bbi.irri.org
IRRI. 2002. Standard Evaluation System for Rice (SES). International Rice Research Institute, Los Banos (Philippines). http://bbi.irri.org
IRRI. 2014. Standard Evaluation System for rice (SES), 5th Edition. International Rice Research Institute, Los Banos (Philippines). http://bbi.irri.org
Jena, K.K. & Mackill, D.J. 2008. Molecular markers and their use in marker-assisted selection in rice. Crop Science, 48(4): 1266-1276. DOI: https://doi.org/10.2135/cropsci2008.02.0082
Jones, J.D. & Dangl, J.L. 2006. The plant immune system. Nature, 444(7117): 323-329. DOI: https://doi.org/10.1038/nature05286
McCouch, S.R., Kochert, G., Yu, Z.H., Wang, Z.Y., Khush, G.S., Coffman, W.R. & Tanksley, S.D. 1988. Molecular mapping of rice chromosomes. Theoretical and Applied Genetics, 76: 815-829. DOI: https://doi.org/10.1007/BF00273666
MINITAB. 2021. Version 21.1. Pennsylvania: Minitab, LLC.
Mohd Ikmal, A., Noraziyah, A.A.S. & Wickneswari, R. 2021. Incorporating drought and submergence tolerance QTL in rice (Oryza sativa L.)-The effect under reproductive stage drought and vegetative stage submergence stresses. Plants, 10: 225. DOI: https://doi.org/10.3390/plants10020225
Nachimuthu, V.V., Muthurajan, R., Duraialaguraja, S., Sivakami, R., Pandian, B.A., Ponniah, G. & Sabariappan, R. 2015. Analysis of population structure and genetic diversity in rice germplasm using SSR markers: an initiative towards association mapping of agronomic traits in Oryza sativa. Rice, 8(1): 1-25. DOI: https://doi.org/10.1186/s12284-015-0062-5
Neeraja, C.N., Maghirang-Rodriguez, R., Pamplona, A., Heuer, S., Collard, B.C., Septiningsih, E.M. & Mackill, D.J. 2007. A marker-assisted backcross approach for developing submergence-tolerant rice cultivars. Theoretical and Applied Genetics, 115: 767-776. DOI: https://doi.org/10.1007/s00122-007-0607-0
Ning, X.I.A.O., Yunyu, W. & Aihong, L. 2020. Strategy for use of rice blast resistance genes in rice molecular breeding. Rice Science, 27(4): 263-277. DOI: https://doi.org/10.1016/j.rsci.2020.05.003
Noraziyah, A.A.S., Swamy, B.P.M., Wickneswari, R., Sta. Cruz, M.T., Raman, A. & Kumar, A. 2016a. Marker assisted pyramiding of drought yield QTLs into a popular Malaysian rice cultivar, MR219. BMC Genetics, 17:1-14. DOI: https://doi.org/10.1186/s12863-016-0334-0
Noraziyah, A.A.S., Swamy, B.P.M., Wickneswari, R., Sta. Cruz, M.T., Sandhu, N., Raman, A.K. & Kumar, A. 2016b. Pyramiding of drought yield QTLs into a high quality Malaysian rice cultivar MRQ74 improves yield under reproductive stage drought. Rice, 9(1):1-13. DOI: https://doi.org/10.1186/s12284-016-0093-6
Oladosu, Y., Rafii, M.Y., Arolu, F., Chukwu, S.C., Muhammad, I., Kareem, I. & Arolu, I.W. 2020. Submergence tolerance in rice: Review of mechanism, breeding and, future prospects. Sustainability, 12(4): 1632. DOI: https://doi.org/10.3390/su12041632
Pradhan, S.K., Nayak, D.K., Mohanty, S., Behera, L. & Barik, S.R. 2015. Pyramiding of three bacterial blight resistance genes for broad-spectrum resistance in deepwater rice variety, Jalmagna. Rice 8(19): 1-14. DOI: https://doi.org/10.1186/s12284-015-0051-8
Prusty, N., Pradhan, B., Deepa, K., Chattopadhyay, B.C., Patra & Sarkar, R.K. 2018. Novel rice (Oryza sativa L.) genotypes tolerant to combined effect of submergence and salt stress. Indian Journal of Plant Genetic and Resources, 31(3): 260-269. DOI: https://doi.org/10.5958/0976-1926.2018.00030.X
R Studio Team. 2015. R Studio: Integrated Development Environment for R. Boston, MA. http://www.rstudio.com/
Ranjith, P., Sahu, S., Dash, S.K., Bastia, D.N. & Pradhan, B.D. 2018. Genetic diversity studies in rice (Oryza sativa L.). Journal of Pharmacognosy and Phytochemistry, 7(2): 2529-2531.
Septiningsih, E.M., Pamplona, A.M., Sanchez, D.L., Neeraja, C.N., Vergara, G.V. & Heuer, S. 2009. Development of submergence-tolerant rice cultivars: The Sub1 locus and beyond. Annals of Botany, 103: 151-160. DOI: https://doi.org/10.1093/aob/mcn206
Sharma, T.R., Madhav, M.S., Singh, B.K., Shanker, P., Jana, T.K. & Dalal, V. 2005. High-resolution mapping, cloning, and molecular characterization of the Pi-k(h) gene of rice, which confers resistance to Magnaporthe grisea. Molecular Genetics and Genomics, 274: 569-578. DOI: https://doi.org/10.1007/s00438-005-0035-2
Singh, R., Dangol, S., Chen, Y.F., Choi, J., Cho, Y.S., Lee, J.E., Choi, M.O. & Jwa, N.S. 2016. Magnaporthe oryzae effector AVR-Pii helps to establish compatibility by inhibition of the rice NADP-malic enzyme resulting in disruption of oxidative burst and host innate immunity. Molecular Cells, 39(5): 426-438. DOI: https://doi.org/10.14348/molcells.2016.0094
Takahashi, A., Hayashi, N., Miyao, A. & Hirochika, H. 2010. Unique feature of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging. BMC Plant Biology, 10: 175. DOI: https://doi.org/10.1186/1471-2229-10-175
Tan, S., Zhu, M. & Zhang, Q. 2010. Physiological responses of bermudagrass (Cynodon dactylon) to submergence. Actaphysiologiae Plantarum, 32:133- 140. DOI: https://doi.org/10.1007/s11738-009-0388-y
Travis, A.J., Norton, G.J., Datta, S., Sarma, R., Dasgupta, T., Savio, F.L. & Price, A.H. 2015. Assessing the genetic diversity of rice originating from Bangladesh, Assam and West Bengal. Rice, 8: 1-9. DOI: https://doi.org/10.1186/s12284-015-0068-z
Winkel, A., Colmer, T.D., Ismail, A.M. & Pedersen, O. 2013. Internal aeration of paddy field rice (Oryza sativa) during complete submergence-importance of light and floodwater O2. New Phytologist, 197:1193-1203. DOI: https://doi.org/10.1111/nph.12048
Wu, Y.Y., Yu, L., Pan, C.H., Dai, Z.Y., Li, Y.H., Xiao, N. & Li, A.H. 2016. Development of near-isogenic lines with different alleles of Piz locus and analysis of their breeding effect under Yangdao 6 background. Molecular Breeding, 36(2): 1-12. DOI: https://doi.org/10.1007/s11032-016-0433-7
Yu, Z.H., Mackill, D.J., Bonman, J.M. & Tanksley, S.D. 1991. Tagging genes for blast resistance in rice via linkage to RFLP markers. Theoretical and Applied Genetics, 81: 471-476. DOI: https://doi.org/10.1007/BF00219436
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