Physicochemical, Microstructural, and Sensory Attributes of Frozen Buffalo Loins Treated with Sodium Tripolyphosphate and Sous-Vide Cooking

https://doi.org/10.55230/mabjournal.v54i2.3252

Authors

  • Ahmad Luthfi Puzelan Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia
  • Nur Raudhatul Syahindah Mohd Radzi Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia
  • Ishamri Ismail Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia

Keywords:

Buffalo, PCA, SEM, sensory evaluation, sodium tripolyphosphate, sous-vide

Abstract

This study examines the effects of sous-vide cooking with varying concentrations of sodium tripolyphosphate (STPP) on the physicochemical, microstructural, and sensory characteristics of frozen buffalo loins. Samples were prepared with 0.15% and 0.30% STPP, alongside a control group without STPP, and cooked using a two-stage sous-vide process at 45°C for 3 hr, followed by 60°C for an additional 3 h. The results demonstrated that STPP significantly influenced pH, cooking loss, water-holding capacity (WHC), colour properties, Warner-Bratzler shear force (WBSF), microstructure, and sensory characteristics. Raising STPP concentration improved WHC and lowered cooking loss. STPP generated a pH rise, which delayed the denaturation of myoglobin and resulted in the sous-vide-cooked buffalo loins having a redder appearance. Scanning electron micrograph (SEM) supported the notable increase in tenderness with STPP addition, by showing structural changes in the muscle fibres; 0.3% of STPP samples displayed noticeably looser fibres and a thinner perimysium. The 0.3% STPP samples had a higher overall acceptability, texture, juiciness, and appearance score than the other samples, as indicated by the sensory evaluation using generic descriptive analysis (GDA) and principal component analysis (PCA). Therefore, it is recommended to incorporate 0.3% STPP into the sous-vide cooking process to optimize the eating quality of the frozen buffalo loins.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Ahn, D.U. & Maurer, A.J. 1989. Effects of added pigments, salt, and phosphate on color, extractable pigment, total pigment, and oxidation-reduction potential in turkey breast meat. Poultry Science, 68(8): 1088-1099. DOI: https://doi.org/10.3382/ps.0681088

AMSA. 1995. American Meat Science Association in cooperation with National Live Stock and Meat Board: Research guidelines for cookery, sensory evaluation and instrumental tenderness measurements of fresh meat, Chicago, Illinois.

Ayub, H. & Ahmad, A. 2019. Physiochemical changes in sous-vide and conventionally cooked meat. International Journal of Gastronomy and Food Science, 17: 100145. DOI: https://doi.org/10.1016/j.ijgfs.2019.100145

Boland, M., Kaur, L., Chian, F.M. & Astruc, T. 2019. Muscle Proteins. Encyclopedia of Food Chemistry. L. Melton, F. Shahidi and P. Varelis. Academic Press, Oxford: 164-179. DOI: https://doi.org/10.1016/B978-0-08-100596-5.21602-8

Cheng, J.-H. & Ockerman, H.W. 2003. Effect of phosphate with tumbling on lipid oxidation of precooked roast beef. Meat Science, 65(4): 1353-1359. DOI: https://doi.org/10.1016/S0309-1740(03)00057-3

Chian, F.M., Kaur, L., Astruc, T., Vénien, A., Stübler, A.-S., Aganovic, K., Loison, O., Hodgkinson, S. & Boland, M. 2021. Shockwave processing of beef brisket in conjunction with sous vide cooking: Effects on protein structural characteristics and muscle microstructure. Food Chemistry, 343: 128500. DOI: https://doi.org/10.1016/j.foodchem.2020.128500

Dang, D.S., Bastarrachea, L.J., Martini, S. & Matarneh, S.K. 2021. Crystallization behavior and quality of frozen meat. Foods, 10(11): 2707. DOI: https://doi.org/10.3390/foods10112707

Food-Act-281. 2023. Law of Malaysia: Food Act and Regulations Act 281. MDC Publisher Sdn Bhd, Ulu Kelang, Kuala Lumpur.

Glorieux, S., Goemaere, O., Steen, L. & Fraeye, I. 2017. Phosphate reduction in emulsified meat products: impact of phosphate type and dosage on quality characteristics. Food Technology and Biotechnology, 55(3): 390-397. DOI: https://doi.org/10.17113/ftb.55.03.17.5089

Hunt, M.C., Sørheim, O. & Slinde, E. 1999. Color and heat denaturation of myoglobin forms in ground beef. Journal of Food Science, 64(5): 847-851. DOI: https://doi.org/10.1111/j.1365-2621.1999.tb15925.x

Ismail, I., Hwang, Y.-H. & Joo, S.-T. 2019. Interventions of two-stage thermal sous-vide cooking on the toughness of beef semitendinosus. Meat Science, 157: 107882. DOI: https://doi.org/10.1016/j.meatsci.2019.107882

Joo, S.T. 2018. Determination of water-holding capacity of porcine musculature based on released water method using optimal load. Korean Journal for Food Science of Animal Resources, 38: 823-828.

Lawrie, R.A. 2006. Lawrie’s Meat Science. Woodhead Publishing Limited, Royston Road, Duxford, United Kingdom. DOI: https://doi.org/10.1533/9781845691615

Li, C., Wang, D., Xu, W., Gao, F. & Zhou, G. 2013. Effect of final cooked temperature on tenderness, protein solubility and microstructure of duck breast muscle. LWT - Food Science and Technology, 51(1): 266-274. DOI: https://doi.org/10.1016/j.lwt.2012.10.003

Morris, C.S., Campbell, Y.L., Smith, B.S., Campano, S.G., Williams, J.B., Kim, T., Dinh, T.T. & Schilling, W. 2019. Utilization of phosphate alternatives in marinated chicken breast and chunked and formed deli ham. Meat and Muscle Biology, 3(1): 500-509. DOI: https://doi.org/10.22175/mmb2019.08.0038

Myhrvold, N., Young, C. & Bilet, M. 2011. Modernist cuisine: The art and science of cooking. The Cooking Lab, Bellevue, WA.

Naveena, B.M. & Kiran, M. 2014. Buffalo meat quality, composition, and processing characteristics: Contribution to the global economy and nutritional security. Animal Frontiers, 4(4): 18-24. DOI: https://doi.org/10.2527/af.2014-0029

Neath, K.E., Del Barrio, A.N., Lapitan, R.M., Herrera, J.R.V., Cruz, L.C., Fujihara, T., Muroya, S., Chikuni, K., Hirabayashi, M. & Kanai, Y. 2007. Difference in tenderness and pH decline between water buffalo meat and beef during postmortem aging. Meat Science, 75(3): 499-505. DOI: https://doi.org/10.1016/j.meatsci.2006.08.016

Nik Zainal Abidin, N.Z., Shamsuddin, N.A., Mohd Radzi, N.R.S., Yew Huat, J.T. & Ishamri, I. 2024. Phosphate and two-stage cooking: Impact on lipid oxidation and tenderness in sous-vide beef semitendinosus. Food Science and Technology International, 0(0). DOI: https://doi.org/10.1177/10820132241299840

Nowak, D. & Jakubczyk, E. 2020. The freeze-drying of foods-the characteristic of the process course and the effect of its parameters on the physical properties of food materials. Foods, 9(10): 1488. DOI: https://doi.org/10.3390/foods9101488

Önenç, A. & Kaya, A. 2004. The effects of electrical stunning and percussive captive bolt stunning on meat quality of cattle processed by Turkish slaughter procedures. Meat Science, 66(4): 809-815. DOI: https://doi.org/10.1016/S0309-1740(03)00191-8

Roldán, M., Antequera, T., Pérez-Palacios, T. & Ruiz, J. 2014. Effect of added phosphate and type of cooking method on physico-chemical and sensory features of cooked lamb loins. Meat Science, 97(1): 69-75. DOI: https://doi.org/10.1016/j.meatsci.2014.01.012

Ruslan, N.N., Tang, J.Y.H., Huda, N., Ismail-Fitry, M.R. & Ishamri, I. 2023. Effects of phosphate and two-stage sous-vide cooking on textural properties of the beef semitendinosus. Food Science of Animal Resources, 43(3): 491-501. DOI: https://doi.org/10.5851/kosfa.2023.e11

Sharma, I., Arora, K., Kumar, S., Bhoi, P. & Vatta, K. 2023. Global trade competitiveness of Indian buffalo meat: Trends and determinants. Buffalo Bulletin, 42(1): 105-124. DOI: https://doi.org/10.56825/bufbu.2023.4214974

Shi, H., Shahidi, F., Wang, J., Huang, Y., Zou, Y., Xu, W. & Wang, D. 2021. Techniques for postmortem tenderisation in meat processing: effectiveness, application and possible mechanisms. Food Production, Processing and Nutrition, 3(1): 21. DOI: https://doi.org/10.1186/s43014-021-00062-0

Trbovich, V., Garza, H., Wick, M., England, E. & Garcia, L. 2017. Investigating the effects of sous vide cooking on tenderness and protein concentration in young fed and cow semitendinosus muscles. Meat and Muscle Biology, 1: 34-34. DOI: https://doi.org/10.22175/rmc2016.033

Valin, C., Pinkas, A., Dragnev, H., Boikovski, S. & Polikronov, D. 1984. Comparative study of buffalo meat and beef. Meat Science, 10(1): 69-84. DOI: https://doi.org/10.1016/0309-1740(84)90032-9

Vaudagna, S.R., Pazos, A.A., Guidi, S.M., Sanchez, G., Carp, D.J. & Gonzalez, C.B. 2008. Effect of salt addition on sous vide cooked whole beef muscles from Argentina. Meat Science, 79(3): 470-482. DOI: https://doi.org/10.1016/j.meatsci.2007.11.001

Zhang, Y.-m., Hopkins, D.L., Zhao, X.-x., van de Ven, R., Mao, Y.-w., Zhu, L.-x., Han, G.-x. & Luo, X. 2018. Characterisation of pH decline and meat color development of beef carcasses during the early postmortem period in a Chinese beef cattle abattoir. Journal of Integrative Agriculture, 17(7): 1691-1695. DOI: https://doi.org/10.1016/S2095-3119(17)61890-2

Published

30-06-2025

How to Cite

Puzelan, A. L., Mohd Radzi , N. R. S., & Ismail, I. (2025). Physicochemical, Microstructural, and Sensory Attributes of Frozen Buffalo Loins Treated with Sodium Tripolyphosphate and Sous-Vide Cooking. Malaysian Applied Biology, 54(2), 39–45. https://doi.org/10.55230/mabjournal.v54i2.3252

Issue

Section

Research Articles

Funding data