ANTIFUNGAL ACTIVITY OF DICHLOROMETHANE AND HEXANE EXTRACTS OF FOUR MALAYSIAN SEAWEED SPECIES AGAINST Ganoderma boninense

Abstract

Ganoderma boninense is a major oil palm fungal pathogen that caused basal stem rot disease and serious efforts are required to identify alternative methods to control this disease. To date, little attempt was done to explore the antifungal potential of bioactive compounds in seaweeds. This study reported the antifungal activity of seaweed extracts against G. boninense. Seaweeds _cfSargassum oligocystum, Caulerpa racemosa var. lamourouxii, Caulerpa racemosa and _cfHalimeda macrophysa were collected from Port Dickson, Malaysia and extracted using dichloromethane and hexane. The antifungal activity assay towards G. boninense was carried out using the poisoned food technique followed by gas chromatography-mass spectrometry (GC-MS) to screen the compounds in the seaweed extracts. Our findings revealed that C. racemosa var. lamourouxii- dichloromethane extract exhibited the highest antifungal activity at a concentration of 0.25 mg/mL with 46.82% inhibition of

1. boninense’s growth followed by C. racemosa var. lamourouxii-hexane extract with 36.43% inhibition. Phytol and tetradecanoic acid were found to be the dominant compounds in the extracts and further analysis of phytol standard proved its antifungal activity. This study highlights the potential of local Malaysian seaweed species as a source of natural and powerful antifungal compounds which could be useful for alternative oil palm disease control in Malaysia.