The Performances of Hybrid Grouper, Epinephelus fuscoguttatus × E. lanceolatus Fed with Defatted Soybean Meal-Based Feeds with Supplementation of Phytase
Keywords:defatted soybean meal, fish performance, hybrid grouper, phytase
Fish meal is the primary protein ingredient in fish feed and this material is expensive and non-renewable. However, the use of plant protein in marine fish feed such as soybean meal products are limited due to anti-nutritional factor that hindered fish performances. Therefore, hybrid grouper, a cross between tiger grouper (Epinephelus fuscoguttatus) and giant grouper (E. lanceolatus) was evaluated using defatted soybean meal (DSM) based feed and phytase. DSM- based feeds (30% of protein replacement) with supplementation of phytase at 0 and 2000 FTU/kg dosages, as well as a control feed (CON) comprised completely of fish meal (FM) was given to the hybrid grouper with an initial body weight of 6.2±0.0g. Each experimental feed was formulated with 50% of crude protein and 12% of crude lipid. The fish were raised in 100L fiberglass tanks equipped with a flow-through water system for each triplicate treatment. The hybrid grouper was fed with the respective feeds twice a day until the perceived satiation level for 12 weeks. Hybrid grouper fed CON and DSM-based feed did not exhibit any significant difference in growth. However, hybrid grouper fed with DSM-based feed grew larger compared to that fed with CON. Hybrid grouper fed with DSM-based feed showed significantly higher feed intake, lower net protein utilization, hepatosomatic index, viscerosomatic index, and apparent digestibility coefficient (ADC) protein values (p<0.05). The value of ADC of phosphorus was slightly higher in DSM-based feed supplemented with phytase at a dosage of 2000 FTU/kg. No significant effect was observed on body proximate composition, morphological condition of the intestine, and ADC of lipids in all the treatments. The results show that hybrid grouper can effectively consume 30% DSM-based feed and supplementing phytase did not affect the fish performances.
Anthonius, A., Yong, A.S.K. & Ching F.F. 2017. Supplementation of duckweed diet and citric acid on growth performance feed utilization digestibility and phosphorus utilization of hybrid grouper, TGGG (Epinephelus fuscoguttatus x Epinephelus lanceolatus) juvenile. Songklanakarin Journal of Science and Technology, 40(5): 1009–1016.
AOAC (Association of Official Analytical Chemists). 1999. In: Official Methods of Analysis of AOAC International. 16th Ed. P. Cuniff (Ed.). Association of Office Analytical Chemists International, Washington.
Άvila, D.S., Sánchez, E.A., Hernández, L.H.H., Araiza, M.A.F & López, O.A. 2015. Addition of yeast and/or phytase to diets with soybean meal as main protein source: Effects on growth, P excretion and lysozyme activity in juvenile rainbow trout (Oncorhynchus mykiss Walbaum). Turkish Journal of Fisheries and Aquatic Sciences 15: 215-222.
Bancroft, J.D. 2008. Theory and practice of histological techniques. Elsevier Health Sciences. pp. 70.
Baruah, K., Sahu, N.P., Pal, A.K. & Debnath, D. 2004. Dietary phytase: An ideal approach for a cost effective and low-polluting aqua feed. NAGA, World Fish Center, 27: 15–19.
Biswas, A.K., Kaku, H., Ji, S.C., Seoka, M. & Takii, K. 2007. Use of soybean meal and phytase for partial replacement of fish meal in the diet of red sea bream, Pagrus major. Aquaculture, 267: 284–291. DOI: https://doi.org/10.1016/j.aquaculture.2007.01.014
Biswas, A., Araki, H., Sakata, T., Nakamori, T., Kato, K. & Takii, K. 2017. Fish meal replacement by soy protein from soymilk in the diets of red sea bream, Pagrus major. Aquaculture Nutrition, 23(6): 1379-1389. DOI: https://doi.org/10.1111/anu.12513
Cao, L., Wang, W., Yang, C., Yang, Y., Diana, J., Yakupitiyage, A., Luo, Z. & Li, D. 2007. Application of microbial phytase in fish feed. Enzyme Microbiology Technology, 40: 497-507. DOI: https://doi.org/10.1016/j.enzmictec.2007.01.007
Choi, S.M., Wang, X.J. & Bai, S.C. 2004. Dietary dehulled soybean meal as a replacement for fish meal in fingerling and growing olive flounder, Paralichthys olivaceus. Aquaculture Research, 35(4): 410-418. DOI: https://doi.org/10.1111/j.1365-2109.2004.01046.x
Chor, W.K., Lim, L.S., Chong, M., Lu, K.C., Sade, A. & Shapawi, R. 2015. Evaluation of tempeh as a potential alternative protein source in the diets for juvenile tiger grouper, Epinephelus fuscoguttatus. Malaysian Journal of Science, 34(1): 58–68. DOI: https://doi.org/10.22452/mjs.vol34no1.6
Daniel, N. 2018. A review on replacing fish meal in aqua feeds using plant protein sources. International Journal of Fisheries and Aquatic Science, 6(2): 164-179.
Dei, H.K. 2011. Soybean as a feed ingredient for livestock and poultry, recent trends for enhancing the diversity and quality of soybean products. In: Recent Trends for Enhancing the Diversity and Quality of Soybean Products. D. Krezhova (Ed.). pp. 215–226. DOI: https://doi.org/10.5772/17601
Deng, J., Mai, K., Ai, Q., Zhang, W., Wang, X., Xu, W. & Liufu, Z. 2006. Effects of replacing fish meal with soy protein concentrate on feed intake and growth of juvenile Japanese flounder, Paralichthys olivaceus. Aquaculture, 258: 503-513. DOI: https://doi.org/10.1016/j.aquaculture.2006.04.004
Ebi, I., Lal, M.T., Ransangan, J., Yong, A.S.K. & Shapawi, R. 2018. Susceptibility of hybrid grouper (Epinephelus fuscogutattus x Epinephelus lanceolatus) to Vibrio harveyi VHJR7. AACL Bioflux, 11(1): 37-42.
Egsgaard, J. 1948. On the calorimetric determination of phosphorus with ‘Amidol’. Acta Physiologica Scandinavica, 16(2-3): 179–182. DOI: https://doi.org/10.1111/j.1748-1716.1948.tb00537.x
Firdaus, R.F., Lim, L.S., Kawamura, G. & Shapawi, R. 2016. Assessment on the acceptability of hybrid grouper, Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ to soybean meal-based diets. AACL Bioflux, 9(2): 284–290.
Furukawa, A. & Tsukahara, H. 1966. On the acid digestion method for the determination of chromic oxide as an index substance in the study of digestibility of fish diet. Bulletin of the Japanese Society of Scientific Fisheries, 32: 502–506. DOI: https://doi.org/10.2331/suisan.32.502
García-Ortega, A., Kissinger, K.R. & Trushenski, J.T. 2016. Evaluation of fish meal and fish oil replacement by soybean protein and algal meal from Schizochytrium limacinum in diets for giant grouper, Epinephelus lanceolatus. Aquaculture, 452: 1–8. DOI: https://doi.org/10.1016/j.aquaculture.2015.10.020
Hernàndez, M.D., Martínez, F.J., Jover, M., & García, G.B. 2007. Effects of partial replacement of fish meal by soybean meal in sharpsnout seabream, Diplodus puntazzo diet. Aquaculture, 263: 159–167. DOI: https://doi.org/10.1016/j.aquaculture.2006.07.040
Hussain, S.M., Ahmad, N., Javid, A., Shahzad, M.M., Hussain, M., & Arsalan, M. 2017. Effects of phytase and citric acid supplemented corn gluten (30%) meal-based diets on the mineral digestibility of Cirrhinus mrigala fingerlings. Turkish Journal of Fisheries and Aquatic Sciences, 18: 501-507.
Kaushik, S.J., Cravedi, J.P., Lalles, J.P., Sumpter, J., Fauconneau, B. & Laroche, M. 1995. Partial or total replacement of fish meal by soybean protein on growth, protein utilization, potential estrogenic or antigenic effects, cholesterolemia and flesh quality in rainbow trout, Oncorhynchus mykiss. Aquaculture, 133: 257–274. DOI: https://doi.org/10.1016/0044-8486(94)00403-B
Kumar, V., Sinha, A.K., Makkar, H.P.S., Boeck, G.D. & Becker, K. 2012. Phytate and phytase in fish nutrition. Journal of Animal Physiology and Animal Nutrition, 96: 335–364. DOI: https://doi.org/10.1111/j.1439-0396.2011.01169.x
Li, X.Y., Zheng, S.X., Ma, X.K., Cheng, K.M. & Wu, G. 2020. Effects of dietary protein and lipid levels on growth performance, feed utilization, and liver histology of largemouth bass (Micropterus salmoides). Amino Acids, 52: 1043-1061. DOI: https://doi.org/10.1007/s00726-020-02874-9
Lim, S.J., Kim, S.S., Ko, G.Y., Song, J.W., Oh, D.H., Kim, J.D., Kim, J.U., & Lee, K.J. 2011. Fish meal replacement by soybean meal in diets for tiger puffer, Takifugu rubripes. Aquaculture, 313(1-4): 165-170. DOI: https://doi.org/10.1016/j.aquaculture.2011.01.007
Lim, S.R., Choi, S.M., Wang, X.J., Kim, K.W., Shin, I.S., Min, T.S. & Bai, S.C. 2004. Effects of dehulled soybean meal as fish meal replacer in diets for fingerling and growing Korean rockfish, Sebates schlegeli. Aquaculture, 231: 457-468. DOI: https://doi.org/10.1016/j.aquaculture.2003.09.008
Luo, Z., Liu, Y.J., Mai, K.S., Tian, L.X., Liu, D.H. & Tan, X.Y. 2004. Partial replacement of fish meal by soybean proteins in diets for grouper, Epinephelus coioides juvenile. Journal of Fisheries of China, 28: 175–181.
Luo, Z., Liu, Y.J., Mai, K.S., Tian, L.X., Yang, H.J. & Liu, D. 2005. Dietary L-methionine requirement of juvenile grouper, Epinephelus coioides at a constant dietary cystine level. Aquaculture, 249: 409–418. DOI: https://doi.org/10.1016/j.aquaculture.2005.04.030
Maas, R.M., Verdegem, M.C.J., Debnath, S., Marchal, L. & Schrama, J.W. 2021. Effect of enzymes (phytase and xylanase), probiotics (B. amyloliquefaciens) and their combination on growth performance and nutrient utilisation in Nile tilapia. Aquaculture, 533: 736226. DOI: https://doi.org/10.1016/j.aquaculture.2020.736226
Makkar, H.P.S., & Becker, K. 2009. Jatropha curcas, a promising crop for the generation of biodisel and value-added coproducts. European Journal of Lipid Science and Technology, 111: 773-787. DOI: https://doi.org/10.1002/ejlt.200800244
Mohd Faudzi, N., Yong, A.S.K., Shapawi, R., Senoo, S., Biswas, A.K. & Takii, K. 2017. Soy protein concentrate as an alternative in replacement of fish meal in the feeds of hybrid grouper, tiger grouper (Epinephelus fuscoguttatus) x giant grouper (E. lanceolatus) juvenile. Aquaculture Research, 49 (1): 431-441. DOI: https://doi.org/10.1111/are.13474
National Research Council. 2011. Nutrient requirements of fish. National Academy Press, Washington, D.C, USA. 144 pp.
Nguyen, H.P., Do, T.V. & Tran, H.D. 2020. Dietary replacement of fish meal by defatted and fermented soybean meals with taurine supplementation for pompano fish: effects on growth performance, nutrient digestibility, and biological parameters in a long-term feeding period. Journal of Animal Science, 98(12): skaa367. DOI: https://doi.org/10.1093/jas/skaa367
Olivia-Teles, A., Pereira, J.P., Gouveia, A. & Gomes, E. 2001. Utilisation of diets supplemented with microbial phytase by seabass (Dicentrarchus labrax) juveniles. Aquatic Living Resources, 11: 255-259. DOI: https://doi.org/10.1016/S0990-7440(98)80008-9
Ovissipour, M., Benjakul, S., Safari, R. & Motamedzadegan, A. 2010. Fish protein hydrolysates production from yellowfin tuna, Thunnus albacares head using Alcalase and Protamex. International Aquatic Research, 2: 87-95.
Pham, M.A., Lee, K.J., Lim, S.J. & Park, K.H. 2007. Evaluation of cottonseed and soybean meal as partial replacement for fishmeal in diets for juvenile Japanese flounder, Paralichthys olivaceus. Fisheries Science, 73: 760–769. DOI: https://doi.org/10.1111/j.1444-2906.2007.01394.x
Refstie, S., Storebakken, T., Baeverfjord, G. & Roem, A.J. 2001. Long-term protein and lipid growth of Atlantic salmon, Salmo salar fed diets with partial replacement of fish meal by soy protein products at medium or high lipid level. Aquaculture, 193: 91–106. DOI: https://doi.org/10.1016/S0044-8486(00)00473-7
Shapawi, R., Ebi, I. & Yong, A.S.K. 2013a. Soybean meal as a source of protein in formulated diets for tiger grouper, Epinephelus fuscoguttatus juvenile. Part I: Effects on growth, survival, feed utilization and body compositions. Agricultural Sciences, 4: 317–323. DOI: https://doi.org/10.4236/as.2013.47045
Shapawi, R., Ebi, I., Yong, A.S.K., Chong, M., Lu, K.C. & Sade, A. 2013b. Soybean meal as a source of protein in formulated diets for tiger grouper, Epinephelus fuscoguttatus juvenile. Part II. Improving diet performances with phytase supplementation. Agricultural Sciences, 4: 19–24. DOI: https://doi.org/10.4236/as.2013.46A003
Wang, Y.R., Wang, L., Zhang, C.X. & Song, K. 2017. Effects of substituting fishmeal with soybean meal on growth performance and intestinal morphology in orange-spotted grouper (Epinephelus coioides). Aquaculture Reports, 5: 52–57. DOI: https://doi.org/10.1016/j.aqrep.2016.12.005
Wu, M., Lu, S., Wu, X., Jiang, S., Luo, Y., Yao, W. & Jin, Z. 2017. Effects of dietary amino acid patterns on growth, feed utilization and hepatic IGFI, TOR gene expression levels of hybrid grouper, Epinephelus fuscoguttatus ♀ x Epinephelus lanceolatus ♂ juveniles. Aquaculture, 468: 508–514. DOI: https://doi.org/10.1016/j.aquaculture.2016.11.019
Yang, Y.H., Wang, Y.Y., Lu, Y. & Li, Q.Z. 2011. Effect of replacing fish meal with soybean meal on growth, feed utilization and nitrogen and phosphorus excretion on rainbow trout (Oncorhynchus mykiss). Aquaculture International, 19: 405–419. DOI: https://doi.org/10.1007/s10499-010-9359-y
Yong, A.S.K., Ooi, S.Y. & Shapawi, R. 2013. The utilization of soybean meal in formulated diet for marble goby, Oxyeleotris marmoratus. Journal of Agricultural Science, 5: 139–149. DOI: https://doi.org/10.5539/jas.v5n11p139
Yong, A.S.K., Mohd Faudzi, N., Senoo, S. & Shapawi, R. 2019. Optimum level of dietary protein and lipid for hybrid grouper, tiger grouper (Epinephelus fuscoguttatus) x giant grouper (E. lanceolatus). Journal of Sustainability Science and Management, 14(5): 1-15.
Yong, A.S.K., Abang Zamhari, D.N.J., Shapawi, R., Zhuo, L.C. & Lin, Y.H. 2020. Physiological changes of giant grouper (Epinephelus lanceolatus) fed with high plant protein with and without supplementation of organic acid. Aquaculture Reports, 18: 100499. DOI: https://doi.org/10.1016/j.aqrep.2020.100499
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