SOYBEAN AS AN ALTERNATIVE NUTRIENT MEDIUM FOR Bacillus subtilis GROWTH

NURUL HANISAH  ABDUL MALIK; KHANOM  SIMARANI; MUHAMAD AFIQ  AZIZ

doi:10.55230/mabjournal.v51i4.12

Authors

NURUL HANISAH ABDUL MALIK Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
KHANOM SIMARANI Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia
MUHAMAD AFIQ AZIZ
afiqaziz@um.edu.my
Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia

Keywords:

alternative, Bacillus subtilis, growth, soybean, nutrient agar

Abstract

Nutrient agar is a commonly used medium for the isolation and growth of a broad range of microorganisms. The feasibility of using soybean as a base medium in the development of alternative growth media was assessed in this study. Nutrient agar was used as a standard guide to evaluating the performance of the formulated soybean agar. Bacillus subtilis was inoculated and allowed to grow on nutrient agar and soybean agar. Their growth was compared within 24 h after inoculation based on the morphology of individual colonies formed on both media and the pattern of bacterial growth. Our results showed that soybean agar had comparable performance to nutrient agar as the morphological characteristics of B. subtilis colonies formed on both media are generally identical in terms of texture, margin, optical properties, colour, elevation, and shape. However, due to the similar appearance of the bacterial colonies and the soybean agar, the colonies formed on the soybean agar were slightly larger than those formed on nutrient agar. In addition, our findings also revealed that agar strips formed the best soybean agar compared to gelatin and agar powder. Ultimately, this study has shown that locally available soybeans and agar strips can be easily formulated as an alternative to commercial nutrient agar and have great potential for bacteriological research.

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References

Al-Azzauy, A.A.M. & Hassan, A.M. 2011. The beetroot juice as a bacterial growth and maintenance medium for many pathogenic bacteria. Iraqi Journal of Market Research and Consumer Protection, 3(5): 147–161.

Anbu, S., Saranraj, P., Padma, J. & Punithavalli, K. 2017. Fruits peel waste as a novel media for the growth of economically important Fungi. Journal of Pharmacognosy and Phytochemistry, 6(6): 426–428.

Arnarson,A. 2019. Soybeans 101:Nutrition Facts and Health Effects [WWW Document]. URL https://www.healthline.com/nutrition/foods/soybeans#plant-compounds (accessed 11.6.21).

Balan, S.S., Nethaji, R., Sankar, S. & Jayalakshmi, S. 2012. Production of gelatinase enzyme from Bacillus spp isolated from the sediment sample of Porto Novo Coastal sites. Asian Pacific Journal of Tropical Biomedicine, 2(3): S1811–S1816. DOI: https://doi.org/10.1016/S2221-1691(12)60500-0

Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M. & Khurana, S. 2015. Evolution of bacterial and fungal growth media. Bioinformation, 11(4): 182–184. DOI: https://doi.org/10.6026/97320630011182

Bonnet, M., Lagier, J.C., Raoult, D. & Khelaifia, S. 2020. Bacterial culture through selective and non-selective conditions: the evolution of culture media in clinical microbiology. New Microbes and New Infections, 34: 100622. https://doi.org/10.1016/j.nmni.2019.100622 DOI: https://doi.org/10.1016/j.nmni.2019.100622

Borah, A., Mahanta, J. & Narzary, D. 2020. Formulation of cost-effective alternative fungal culture media using local resources. Journal of Advanced Plant Sciences, 10(2): 31–36.

Brufau, G., Boatella, J. & Rafecas, M. 2006. Nuts: source of energy and macronutrients. British Journal of Nutrition, 96(S2): S24–S28. DOI: https://doi.org/10.1017/BJN20061860

Carota, E., Petruccioli, M., D’Annibale, A., Gallo, A.M. & Crognale, S. 2020. Orange peel waste–based liquid medium for biodiesel production by oleaginous yeasts. Applied Microbiology and Biotechnology, 104(10): 4617–4628. DOI: https://doi.org/10.1007/s00253-020-10579-y

Cruz, C.H. da S., Santos, J.B. dos, Santos, F.P. dos, Silva, G.M.M., Cruz, E.F.N. da, Ramos, G.L. de P.A. & Nascimento, J. dos S. 2020. Texturized soy protein as an alternative low-cost media for bacteria cultivation. Bacterial Empire, 3(4): 74–76. DOI: https://doi.org/10.36547/be.2020.3.4.74-76

Darmady, E.M., Hughes, K.E.A., Jones, J.D., Prince, D. & Tuke, W. 1961. Sterilization by dry heat. Journal of Clinical Pathology, 14(1): 38–44. DOI: https://doi.org/10.1136/jcp.14.1.38

Das, N., Triparthi, N., Basu, S., Bose,C., Maitra, S. & Khurana,S. 2015. Progress in the development of gelling agents for improved culturability of microorganisms. Frontiers in Microbiology, 6: 698. DOI: https://doi.org/10.3389/fmicb.2015.00698

Deasy, G.F. 1939. The Soya Bean in Manchuria’. Economic Geography, 15(3): 303–310. DOI: https://doi.org/10.2307/141549

Deivanayaki, M. & Iruthayaraj, A.P. 2012. Alternative vegetable nutrient source for microbial growth. International Journal of Biosciences, 2(5): 47–51.

Haddish, K. 2015. Production of single cell protein from fruit of beles (Opuntia ficus-Indica L.) peels using saccharomyces cerevisiae. Journal of Microbiology & Experimentation, 2(7): 73. DOI: https://doi.org/10.15406/jmen.2015.02.00073

Hanond, P., Samerpitak, K., Kongthawon, A. & Thrakarathai, K. 2007. Application of food grade agar for preparation as media using in microbiological laboratory learning. Srinagarind Medical Journal, 22(4): 376–383.

Hasanin, M.S. & Hashem, A.H. 2020. Eco-friendly, economic fungal universal medium from watermelon peel waste. Journal of Microbiological Methods, 168: 105802. DOI: https://doi.org/10.1016/j.mimet.2019.105802

Iqbal, A., Khalil, I.A., Ateeq, N. & Sayyar Khan, M. 2006. Nutritional quality of important food legumes. Food Chemistry, 97(2): 331–335. DOI: https://doi.org/10.1016/j.foodchem.2005.05.011

Iram, N., Shakir, H.A., Irfan, M., Khan, M., Ali, S., Anwer, A., Saeed, S. & Qazi, J.I. 2021. Statistical optimization of amylase production using grape fruit peels in submerged fermentation. Acta Scientiarum-Technology, 43: 1–11. DOI: https://doi.org/10.4025/actascitechnol.v43i1.50538

Jadhav, P., Sonne, M., Kadam, A., Patil, S., Dahigaonkar, K. & Oberoi, J.K. 2018. Formulation of cost effective alternative bacterial culture media using fruit and vegetables waste. International Journal of Current Research and Review, 10: 2.

Joshi,V. & Kumar,S. 2015. Meat analogues: Plant based alternatives to meat products-A review. International Journal of Food and Fermentation Technology, 5(2): 107. DOI: https://doi.org/10.5958/2277-9396.2016.00001.5

Kouris-Blazos, A. & Belski, R. 2016. Health benefits of legumes and pulses with a focus on Australian sweet lupins. Asia Pacific Journal of Clinical Nutrition, 25(1): 1–17.

Kumbhar, J.V., Rajwade, J.M. & Paknikar, K.M. 2015. Fruit peels support higher yield and superior quality bacterial cellulose production. Applied Microbiology and Biotechnology, 99(16): 6677–6691. DOI: https://doi.org/10.1007/s00253-015-6644-8

Lagier, J.C., Edouard, S., Pagnier, I., Mediannikov, O., Drancourt, M. & Raoult, D. 2015. Current and past strategies for bacterial culture in clinical microbiology. Clinical Microbiology Reviews, 28(1): 208–236. DOI: https://doi.org/10.1128/CMR.00110-14

Ledward, D.A. 2000. Gelatin. In: Handbook of hydrocolloids. G.O. Phillips and P.A. Williams (Eds.). Woodhead Publishing Ltd. pp. 67–86.

Liu, K. 1997. Chemistry and nutritional value of soybean components. In: Soybeans. Springer, Boston, MA. pp. 25–113. DOI: https://doi.org/10.1007/978-1-4615-1763-4_2

Mad-Ali, S., Benjakul, S., Prodpran,T. & Maqsood, S. 2017. Characteristics and gelling properties of gelatin from goat skin as affected by drying methods. Journal of Food Science and Technology, 54(6): 1646–1654. DOI: https://doi.org/10.1007/s13197-017-2597-5

Maphosa, Y. & Jideani, V.A. 2017. The Role of Legumes in Human Nutrition. In: Functional Food - Improve Health through Adequate Food. Intech London. pp. 13. DOI: https://doi.org/10.5772/intechopen.69127

Mohammed, A.A., Al-Mugdadi, S.F.H., Al-Sudani, B.T. & Kamel, J.K. 2018. Beetroot juice as an alternative growth and maintenance medium for six isolates of mycobacterium spp. Journal of Pharmaceutical Sciences and Research, 10(8): 1881–1884.

Mohammed, B.B., Shatti, Z.O., Jasim, E.I., Dari,W.A. & Nasam Alfraji, M.D. 2020. Local culture medium from the legumes mixture as a novel media for the growth and stimulation of prodigiosin pigment which production from Serratia marcescens that isolated environmentally. Plant Archives, 20(1): 991–1000.

Monte Singer, W., Zhang, B., Rouf Mian, M.A. & Huang, H. 2020. Soybean Amino acids in health, genetics, and evaluation. In: Soybean for Human Consumption and Animal Feed. IntechOpen. pp. 14. DOI: https://doi.org/10.5772/intechopen.89497

Mosher, R., Crawford, K. & Lukowiak, A. 2009. A soy-based alternative to traditional bacterial nutrient media. American Biology Teacher, 71(1): 49–51. DOI: https://doi.org/10.2307/27669363

Neal, E. 2019. Organisms That grow on a nutrient agar plate [WWW Document]. Sciencing. URL https://sciencing.com/organisms-grow-nutrient-agar-plate-8094992.html (accessed 30.5.21).

Okpalauwaekwe, E.O., Umedum, C.U., Iheukwumere, I .H. & Akakuru, L.C. 2020. Optimization of mycological media using agro waste for the production of antimicrobial substance. Microbiology Research Journal International, 30(8): 67–73. DOI: https://doi.org/10.9734/mrji/2020/v30i830252

Palaniraj,A. & Jayaraman,V. 2011. Production, recovery and applications of xanthan gum by Xanthomonas campestris. Journal of Food Engineering, 106(1): 1–12. DOI: https://doi.org/10.1016/j.jfoodeng.2011.03.035

Petrovski,S. & Tillett,D. 2012. Back to the kitchen: Food-grade agar is a low-cost alternative to bacteriological agar. Analytical Biochemistry, 429(2): 140–141. DOI: https://doi.org/10.1016/j.ab.2012.07.011

Putri,C.H., Janica,L., Jannah,M. & Ariana,P.P. 2018. Utilization of Dragon Fruit Waste as Microbial Growth Media, in: Proceeding of the 10th SISAK, Daejeon, Korea, pp. 91–95.

Raina, R.J. & Babbar, S.B. 2011. Evaluation of Blends of Alternative Gelling Agents with Agar and Development of Xanthagar, A Gelling Mix, Suitable for Plant Tissue Culture Media. Asian Journal of Biotechnology, 3(2): 153–164. DOI: https://doi.org/10.3923/ajbkr.2011.153.164

Ravimannan, N., Arulanantham, R., Pathmanathan, S. & Niranjan, K. 2014. Alternative culture media for fungal growth using different formulation of protein sources. Annals of Biological Research, 5(1): 36–39.

Ravimannan, N. & Pathmanathan, S. 2016. Soy flour as alternative culture media for yeasts. Global Journal of Science Frontier Research: C Biological Science, 16(3): 75–78.

Sandle, T. 2011. History and development of microbiological culture media. Journal of Institute of Science and Technology, 10–14.

Sandle, T. 2017. Sterilization of Microbiological Culture Media. Journal of GxP Compliance, 21(5): 13–22.

Shareef,S. 2019. Formulation of Alternative Culture Media from Natural Plant Protein Sources for Cultivation of Different Bacteria and Fungi. Zanco Journal of Pure and Applied Sciences, 31(4): 61-69. DOI: https://doi.org/10.21271/zjpas.31.4.7

Umesh, M., Thazeem, B. & Preethi, K. 2019. Valorization of pineapple peels through single cell protein production using saccharomyces cerevisiae NCDC 364. Applied Food Biotechnology, 6(4): 255–263.

Uthayasooriyan, M., Pathmanathan, S., Ravimannan, N. & Sathyaruban, S. 2016. Formulation of alternative culture media for bacterial and fungal growth. Der Pharmacia Lettre, 8(1): 444–449.

Valdez-Calderón, A., Barraza-Salas, M., Quezada-Cruz, M., Islas-Ponce, M.A., Angeles-Padilla, A.F., Carrillo-Ibarra, S., Rodríguez, M., Rojas-Avelizapa, N.G., Garrido-Hernández, A. & Rivas-Castillo, A.M. 2020. Production of polyhydroxybutyrate (PHB) by a novel Klebsiella pneumoniae strain using low-cost media from fruit peel residues. Biomass Conversion and Biorefinery, 12: 1–14. DOI: https://doi.org/10.1007/s13399-020-01147-5

Vieira, I.M.M., Santos, B.L.P., Silva, L.S., Ramos,L.C., de Souza, R.R., Ruzene, D.S. & Silva, D.P. 2021. Potential of pineapple peel in the alternative composition of culture media for biosurfactant production. Environmental Science and Pollution Research, 28(48): 1–15. DOI: https://doi.org/10.1007/s11356-021-15393-1