EFFECTS OF PALM OIL DERIVED TOCOTRIENOL RICH FRACTION AND VITAMIN E ISOMERS ON BIOMARKERS OF EARLY ATHEROGENESIS IN STIMULATED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS

https://doi.org/10.55230/mabjournal.v51i4.23

Authors

  • SUHAILA ABD MUID Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA Sungai Buloh, Selangor, Malaysia; Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia
  • GABRIELE ANISAH RUTH FROEMMING Faculty of Medicine and Health Sciences, UNIMAS, Sarawak, Malaysia.
  • ABD MANAF ALI Faculty of Biotechnology and Agriculture, UNiSZA, Terengganu, Malaysia
  • THUHAIRAH HASRAH ABDUL RAHMAN Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA Sungai Buloh, Selangor, Malaysia; Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia
  • ZALINA HAMID Sime Darby Plantations, Ara Damansara, Petaling Jaya, Selangor, Malaysia
  • HAPIZAH NAWAWI Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA Sungai Buloh, Selangor, Malaysia; Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia

Keywords:

Endothelial activation, inflammation, tocopherol, tocotrienols, vitamin E

Abstract

This study was conducted to investigate the effects of tocotrienol rich fraction (TRF), α-TOC, and pure TCT isomers (α-. γ- & δ-TCT) on inflammation, endothelial activation, nuclear factor kappa B (NFκB), endothelial nitric oxide synthase (eNOS) and monocyte binding activity (MBA) in vitro. Human umbilical vein endothelial cells (HUVECs) were incubated with various concentrations of α-TOC, pure TCT isomers and TRF (0.3-10 µM) together with lipopolysaccharides (LPS) for 16 h. Culture medium and cells were collected and measured for the protein and gene expression of IL-6, TNF-α, NFκB, ICAM-1, VCAM-1, e-selectin, and eNOS. Monocyte binding activity (MBA) was measured by Rose Bengal staining. Area under the curve (AUC) analysis revealed that TRF and pure TCT particularly γ- and δ- isomers, showed better inhibition of inflammation and endothelial activation, MBA and greater eNOS increment than α-TOC. These suggest that TRF and pure TCT isomers have potential as preventive anti-atherogenic agents by attenuating the release of early biomarkers of atherogenesis which is better than α-TOC in LPS-stimulated human endothelial cells.   

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Published

31-10-2022

How to Cite

ABD MUID, S., RUTH FROEMMING, G. A. ., ALI, A. M. ., ABDUL RAHMAN, T. H. ., HAMID, Z. ., & NAWAWI, H. . (2022). EFFECTS OF PALM OIL DERIVED TOCOTRIENOL RICH FRACTION AND VITAMIN E ISOMERS ON BIOMARKERS OF EARLY ATHEROGENESIS IN STIMULATED HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS. Malaysian Applied Biology, 51(4), 145–152. https://doi.org/10.55230/mabjournal.v51i4.23