HDL AND ITS SUBPOPULATION (HDL2 AND HDL3) PROMOTE CHOLESTEROL TRANSPORTERS EXPRESSION AND ATTENUATES INFLAMMATION IN 3T3-L1 MATURE ADIPOCYTES INDUCED BY TUMOR NECROSIS FACTOR ALPHA

SUHAILA ABD MUID; REMEE  AWANG JALIL; NOOR HANISA  HARUN; HAPIZAH  MOHD NAWAWI; GABRIELE ANISAH  RUTH FROEMMING

doi:10.55230/mabjournal.v51i4.24

Authors

SUHAILA ABD MUID
suhaila_muid@uitm.edu.my
Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA Sungai Buloh, Selangor, Malaysia; Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia
REMEE AWANG JALIL Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh, Selangor, Malaysia
NOOR HANISA HARUN Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh, Selangor, Malaysia
HAPIZAH MOHD NAWAWI Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA Sungai Buloh, Selangor, Malaysia; Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh, Selangor, Malaysia
GABRIELE ANISAH RUTH FROEMMING Faculty of Medicine and Health Sciences, UNIMAS, Sarawak, Malaysia.

Keywords:

Adipokines, cholesterol transporters (ABCA1 and SR-B1),, HDL, HDL2, HDL3, Inflamed adipocytes

Abstract

Obesity activates inflammation causing dysfunction of adipocytes. Increasing high-density lipoprotein (HDL) levels in obesity may be beneficial in overcoming this effect. However, not much data is available on the effects of HDL and its subpopulations in inflamed adipocytes. The objective of this study was to investigate the effects of total HDL (tHDL) and the comparison between its subpopulations (HDL2 & HDL3) on protein and gene expression of cholesterol transporters, inflammation, and adipokines in TNF-α stimulated 3T3-L1 mature adipocytes. TNFα alone had lower adiponectin and higher protein and gene expression of IL-6 and NF-ĸβ (p65) compared to unstimulated adipocytes and these effects were attenuated by HDLs especially HDL3 (in most of the biomarkers). HDL and its subpopulation had higher cholesterol transporters expression in 3T3-L1 mature adipocytes induced by TNF-α compared to unstimulated cells. Increment of cholesterol transporters expression by HDL leads to reduce secretion of inflammatory markers [IL-6 & NF-kB (p65)] and visfatin and increases adiponectin secretion in the inflamed mature adipocytes. HDL exhibits beyond its reverse cholesterol transporter property by exhibiting anti-inflammatory effects thru the deactivation of NF-ĸβ (p65). This may contribute to reducing the progression of obesity-related complications.

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