Novel Bioactive Peptides from Red Bigeye (Priacanthus macracanthus) Flesh Protein

Nor Salasiah Mohamed; Amiza Mat Amin; Fisal  Ahmad

doi:10.55230/mabjournal.v53i3.2790

Authors

Nor Salasiah Mohamed Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Food Science and Technology Research Centre, MARDI Kuala Terengganu, KM 10 Jalan Kelantan, Manir, 21200 Kuala Terengganu, Terengganu, Malaysia
Amiza Mat Amin
ama@umt.edu.my
Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Functional Food RIG, Food Security in Changing Climate SIG, Food Security Research Cluster, Universiti Malaysia Terengganu (UMT), 21030 Kuala Nerus, Terengganu, Malaysia
Fisal Ahmad Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia; Functional Food RIG, Food Security in Changing Climate SIG, Food Security Research Cluster, Universiti Malaysia Terengganu (UMT), 21030 Kuala Nerus, Terengganu, Malaysia

Keywords:

Red bigeye, Angiotensin-I-converting enzyme (ACE), Dipeptidyl peptidase-IV (DPP-IV), in-silico, bioactive peptides

Abstract

Red bigeye (Priacanthus macracanthus) is a common fish species in Malaysia. This study reported an in silico assessment of the main proteins in red bigeye flesh as precursors for bioactive peptides. Six major proteins were chosen as precursors from the proteomic profiles of red bigeye proteins. Analyses using the BIOPEP-UWM database found that Protein number 4 gave the highest total number of bioactive peptides (5052 peptides), with dominant bioactivity in angiotensin-I-converting enzyme (ACE) inhibition (1571 peptides) and dipeptidyl peptidase-IV (DPP-IV) inhibition (2238 peptides). The ACE inhibitors had a frequency of bioactive fragment occurrences (A) of 0.4098, while the DPP-IV inhibitors gave a frequency of 0.5805. In silico proteolysis using BIOPEP-UWM found that pepsin (pH > 2) was the most promising proteinase in releasing a high number of DPP-IV and ACE inhibitory peptides. A novel peptide with significant potential was identified as QYKF. This study shows that red bigeye is a potential source of antihypertensive and antidiabetic peptides.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Bao, Y., Wang, H., Li, X., Li, C., & Zhang, Y. 2020. Recent advances in the development of dipeptidyl peptidase IV (DPP-IV) inhibitors for the treatment of type 2 diabetes mellitus. Journal of Medicinal Chemistry, 63(4): 1466-1484.

Borawska-Dziadkiewicz, J., Darewicz, M. & Tarczyńska, A.S. 2021. Properties of peptides released from salmon and carp via simulated human-like gastrointestinal digestion described applying quantitative parameters. PLOS One, 16(8): 1-23. DOI: https://doi.org/10.1371/journal.pone.0255969

Department of Fisheries. 2021. Annual Fisheries Statistics. Department of Fisheries, Malaysia.

Erdmann, K., Cheung, B.W.Y. & Schröder, H. 2018. The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular disease. Journal of Nutritional Biochemistry, 31: 1-10.

Gupta, S., Kapoor, P., Chaudhary, K., Gautam, A., Kumar, R. & Raghava, G.P.S. 2013. In silico approach for predicting toxicity of peptides and proteins. PLOS One, 8(9): e73957. DOI: https://doi.org/10.1371/journal.pone.0073957

Kim, S. K. & Wijesekara, I. 2021. Development and biological activities of marine-derived bioactive peptides: A review. Journal of Functional Foods, 79: 104392.

Kinter, M. & Sherman, N.E. 2005. Protein sequencing and identification using tandem mass spectrometry (Vol. 9). John Wiley & Sons Inc, United States, America.

Kwan, S.H., Baie, S. & Ismail, M.N. 2016. Profiling of proteins and post translational modifications of Channa striatus dried meat. Current Proteomics, 13(1): 9-19. DOI: https://doi.org/10.2174/1570164613666160413123846

Lafarga, T., Connor, P.O. & Hayes, M. 2014. Peptides Identification of novel dipeptidyl peptidase-IV and angiotensin-I-converting enzyme inhibitory peptides from meat proteins using in silico analysis. Peptides, 59: 53-62. DOI: https://doi.org/10.1016/j.peptides.2014.07.005

Lin, K., Zhang, L.W., Han, X., Xin, L., Meng, Z.X., Gong, P.M. & Cheng, D.Y. 2018. Yak milk casein as potential precursor of angiotensin I-converting enzyme inhibitory peptides based on in silico proteolysis. Food Chemistry, 254: 340-347. DOI: https://doi.org/10.1016/j.foodchem.2018.02.051

Malagón-Rojas, J.N., Mantilla-Escalante, D.C. & Sánchez-Castañeda, E. 2020. Bioactive peptides from milk: Focus on the casein hydrolysates. Nutrients, 12(8): 2401.

Marciniak, A., Suwal, S., Naderi, N., Pouliot, Y. & Doyen, A. 2018. Enhancing enzymatic hydrolysis of food proteins and production of bioactive peptides using high hydrostatic pressure technology. Trends in Food Science and Technology, 80: 187-198. DOI: https://doi.org/10.1016/j.tifs.2018.08.013

Minkiewicz, P., Dziuba, J. & Michalska, J. 2011. Bovine meat proteins as potential precursors of biologically active peptides - A computational study based on the BIOPEP database. Food Science and Technology International, 17(1): 39-45. DOI: https://doi.org/10.1177/1082013210368461

Nasri, R., Jridi, M. & Nasri, M. 2019. Advances in seafood by-products valorization: Development of bioactive peptides and applications in food and health. Marine Drugs, 17(3): 124.

Ochiai, Y. & Ozawa, H. 2020. Biochemical and physicochemical characteristics of the major muscle proteins from fish and shellfish. Fisheries Science, 86: 729-740. DOI: https://doi.org/10.1007/s12562-020-01444-y

Paee, K.F., Razali, N., Sarbini, S.R., Nair, R., Nair, R., Tau Len, K.Y. & Norfahana, A.T. 2021. The production of collagen type I hydrolysate derived from Tilapia (Oreochromis sp.) skin using thermolase PC10F and its in silico analysis. Food Biotechnology, 35(1): 1-21. DOI: https://doi.org/10.1080/08905436.2020.1869040

Sampath Kumar, N.S., Nazeer, R.A. & Jaiganesh, R. 2020. Bioactive peptides: Applications in foods and health benefits. In Marine Nutraceuticals (pp. 243-272). Academic Press.

Shaviklo, A.R. 2013. Development of fish protein powder as an ingredient for food applications: A review. Journal of Food Science and Technology, 52(2): 648-661. DOI: https://doi.org/10.1007/s13197-013-1042-7

Sousa, G.T., Lira, F.S., Rosa, J.C., de Oliveira, E.P., Oyama, L.M. & Santos, R.V. 2019. The role of exercise training on immune response in obesity. Journal of Cellular Physiology, 234(12): 19488-19499.

Tejano, L.A., Peralta, J.P., Yap, E.E.S., Panjaitan, F.C.A. & Chang, Y.W. 2019. Prediction of bioactive peptides from Chlorella sorokiniana proteins using proteomic techniques in combination with bioinformatics analyses. International Journal of Molecular Sciences, 20; 1786. DOI: https://doi.org/10.3390/ijms20071786

Tu, M., Liu, H., Zhang, R. Chen, H., Fan, F., Shi, P., Xu, X., Lua, W. & Dua, M. 2018. Bioactive hydrolysates from casein: Generation, identification, and in silico aoxicity and allergenicity prediction of peptides. Journal of the Science of Food and Agriculture, 98(9): 3416-3426. DOI: https://doi.org/10.1002/jsfa.8854

Yu, Z., Wu, S., Zhao, W., Ding, L., Li, J. & Liu, J. 2019. Virtual screening and molecular docking for exploring ACE inhibitory peptides in Larimichthys crocea nebulin protein. International Food Research Journal, 26(5): 1417-1426.

Zhao, M., Su, G., Li, Y., Ma, Y. & Guo, L. 2020. Encapsulation of bioactive peptides: A strategy to improve stability, bioavailability, and functionality. Journal of Agricultural and Food Chemistry, 68(5): 1290-1300.