Isolation of Lactic Acid Bacteria (LAB) from Mimosa pudica (Semalu) for Production of Bacterial Cellulose

Lim Bei Min; Junaidi Zakaria; Siti Hatijah Mortan; Shahril  Mohamad; Mohd Hairul Ab Rahim

doi:10.55230/mabjournal.v52i4.a050

Authors

Lim Bei Min Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
Junaidi Zakaria
junaidibz@umpsa.edu.my
Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
Siti Hatijah Mortan Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
Shahril Mohamad Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
Mohd Hairul Ab Rahim Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia

Keywords:

Bacterial cellulose, lactic acid bacteria, medicinal plant, microfibril, Mimosa pudica

Abstract

Bacterial cellulose (BC) is a potential eco-friendly biopolymer. BC has higher crystallinity and purity compared to plant cellulose. Scientific studies on the production of BC from lactic acid bacteria (LAB) are minimal compared to other common bacteria such as Acetobacter xylinum. LAB was screened and isolated from different tissues of Mimosa pudica (medicinal plant) using MRS broth and agar as the selective medium. LAB isolates were subjected to 16S rRNA gene sequencing of all the bacterial isolates. BC was produced from all LAB isolates by incubating at 30 °C for 14 days in herbal tea medium (Strobilanthes crispus) and HS medium (control) with 130 r.p.m agitation. BC produced by two selected bacterial isolates was characterized using FESEM, FTIR, XRD, and TGA. Molecular analysis of the 16S rRNA gene of all the potential LAB isolates shows 99.86 - 100% identity to 16S rRNA sequences of other Lactobacillus plantarum strains. Two selected L. plantarum strains (LBM001 & LBM004) produce BC in sphere-like particles with a 1.4 to 2.2 µm diameter range of microfiber. FTIR analysis shows that BC produced by LBM001 and LBM004 have four similar cellulose regions identified in cellulose from other sources, which are O-H stretch (3400-330 cm^-1), C-H stretch (2970-2800 cm^-1), O-H bending (1620cm^-1) and C-O-C stretch (1100-1073 cm^-1). XRD analysis shows BC produced by the L. plantarum strains consists of two different XRD peaks at the 2θ angle of 21.53° and 21.85° instead of a single peak (22.76°) identified in the BC produced by A. xylinum and plant cellulose. A similar TG and DTG curved pattern was detected in the BC produced by the L. plantarum strains with the BC produced by A. xylinum and plant cellulose. The LAB isolates from M. pudica have potential in BC production based on the multiple characterization studies.

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References

Andritsou, V., de Melo, E.M., Tsouko, E., Ladakis, D., Maragkoudaki, S., Koutinas, A.A. & Matharu, A.S. 2018. Synthesis and characterization of bacterial cellulose from citrus-based sustainable resources. ACS Omega, 3(8): 10365-10373.

Baharuddin, N.S., Roslan, M., Bawzer, M., Azzeme, A.M., Rahman, Z.A., Khayat, M.E., Rahman, N. & Sobri, Z.M. 2021. Response surface optimization of extraction conditions and in vitro antioxidant and antidiabetic evaluation of an under-valued medicinal weed, Mimosa pudica. Plants, 10(8): 1692.

Barud, H.S., Ribeiro, C.A., Crespi, M.S., Martines, M.A.U., Dexpert-Ghys, J., Marques, R.F.C., Messaddeq, Y. & Ribeiro, S.J.L. 2007. Thermal characterization of bacterial cellulose-phosphate composite membranes. Journal of Thermal Analysis and Calorimetry, 87: 815-818.

Chang, C.P., Wang, I.C., Hung, K.J., & Perng, Y.S. 2010. Preparation and characterization of nanocrystalline cellulose by acid hydrolysis of cotton linter. Taiwan Journal for Science, 25(3): 251-264.

Chua, G.K., Tan, F.H.Y., Chew, F.N. & Mohd-Hairul, A.R. 2019. Nutrients content of food wastes from different sources and its pre-treatment. AIP Conference Proceedings, 2124(1): 020031.

Chua, G.K., Tan, F.H.Y., Chew, F.N., Mohd-Hairul, A.R. & Ahmad, M.A.A. 2020. Food waste hydrolysate as fermentation medium: Comparison of pre-treatment methods. Materials Today: Proceedings, 42: 131-137.

de Assis, C.A., Houtman, C., Phillips, R., Bilek, E.M.T., Rojas, O.J., Pal, L., Peresin, M.S., Jameel, H. & Gonzalez, R. 2017. Conversion economics of forest biomaterials: Risk and financial analysis of CNC Manufacturing. Biofuels, Bioproducts and Biorefining, 11: 682-700.

de France, K.J., Hoare, T. & Cranston, E.D. 2017. Review of hydrogels and aerogels containing nanocellulose. Chemistry of Materials, 29: 4609-4631.

de Melo, E.M., Clark, J.H. & Matharu, A.S. 2017. The Hy-MASS concept: hydrothermal microwave assisted selective scissoring of cellulose for in situ production of (meso)porous nanocellulose fibrils and crystals. Green Chemistry, 19: 3408-3417.

Fang, T.T., Mortan, S.H., Mei, L.C., Adzahar, N.S. & Mohd-Hairul, A.R. 2019. Isolation and identification of lactic acid bacteria from selected local medicinal plants. Malaysian Journal of Biochemistry and Molecular Biology, 1: 106-109.

Fasoli, M., Dell'Anna, R., Dal Santo, S., Balestrini, R., Sanson, A., Pezzotti, M., Monti, F. & Zenoni, S. 2016. Pectins, hemicelluloses and celluloses show specific dynamics in the internal and external surfaces of grape berry skin during ripening. Plant Cell Physiology, 57(6): 1332-1349.

Gea, S., Reynolds, C.T., Roohpour, N., Wirjosentono, B., Soykeabkaew, N., Bilotti, E., & Peijs, T. 2011. Investigation into the structural, morphological, mechanical and thermal behaviour of bacterial cellulose after a two-step purification process. Bioresource Technology, 102(19): 9105-9110.

Gong, J., Li, J., Xu, J., Xiang, Z. & Mo, L. 2017. Research on cellulose nanocrystals produced from cellulose sources with various polymorphs. RSC Advances, 7: 33486-33493.

Hashim, N.A.R.N.A., Zakaria, J., Mohamad, S., Mohamad, S.F.S. & Rahim, M.H.A. 2021. Effect of different treatment methods on the purification of bacterial cellulose produced from OPF juice by Acetobacter xylinum. IOP Conference Series: Materials Science and Engineering, 1092(1): 012058.

Hospodarova, V., Singovszka, E. & Stevulova, N. 2018. Characterization of cellulosic fibers by FTIR spectroscopy for their further implementation to building materials. American Journal of Analytical Chemistry, 9: 303-310.

Ismail, M.F., Sabri, N.A., Tajuddin, S.N., Mei, L.C., Mortan, S.H. & Mohd-Hairul, A.R. 2019. Isolation and identification of denitrifying bacteria from indigenous microorganisms. Malaysian Journal of Biochemistry and Molecular Biology, 1: 17-21.

Jagetia, G.C. & Vanlalhruaii, F. 2020. Anticancer potential of Mimosa pudica Linn. Lajwanti in cultured Dalton's ascites lymphoma cells. International Journal of Complementary and Alternative Medicine, 13(3): 91-94.

Javier-Astete, R., Jimenez-Davalos, J. & Zolla, G. 2020. Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.) K. Schum. and Guazuma crinita Lam. PLoS ONE, 16(10): e0256559.

Khan, H., Kadam, A. & Dutt, D. 2020. Studies on bacterial cellulose produced by a novel strain of Lactobacillus genus. Carbohydrate Polymers, 229: 115513.

Klemm, D., Heublein, B., Fink, H.P. & Bohn, A. 2005. Cellulose: Fascinating biopolymer and sustainable raw material. Angewandte Chemie International Edition, 44: 3358-3393.

Kumar, A. & Kumar, D. 2014. Isolation and characterization of bacteria from dairy samples of Solan in Himachal Pradesh for identification of Lactobacillus spp. International Journal of Pharmaceutical Sciences Review and Research, 25(110): e114.

Lan, P.T., Huyen, N.T.N., Kim, S,Y., Hang, P.T.N. & Tung, B.T. 2021. Phytochemical analysis and protective effect of ethanolic extract of Mimosa pudica Linn. on methylglyoxal-induced glucotoxicity. Journal of Applied Pharmaceutical Science, 11(9): 93-101.

Manfredi, L.B., Rodríguez, E.S., Wladyka-Przybylak, M., & Vázquez, A. 2006. Thermal degradation and fire resistance of unsaturated polyester, modified acrylic resins and their composites with natural fibres. Polymer degradation and stability, 91(2): 255-261.

Sani, A. & Dahman, Y. 2010. Improvements in the production of bacterial synthesized biocellulose nanofibres using different culture methods. Journal of Chemical Technology & Biotechnology, 85(2): 151-164.

Shi, Z., Zhang, Y., Phillips, G.O. & Yang, G. 2014. Utilization of bacterial cellulose in food. Food Hydrocolloids, 35: 539-545.

Siró, I. & Plackett, D. 2010. Microfibrillated cellulose and new nanocomposite materials: A review. Cellulose, 17: 459-494.

Sumardee, N.S.J., Mohd-Hairul, A.R.& Mortan, S.H. 2020. Effect of inoculum size and glucose concentration for bacterial cellulose production by Lactobacillus acidophilus. IOP Conference Series: Materials Science and Engineering, 991(1): 012054.

Sun, Y., Lin, L., Deng, H., Li, J., He, B., Sun, R. & Ouyang, P. 2008. Structural changes of bamboo cellulose in formic acid. BioResources, 3(2): 297-315.

Tajima, K., Kusumoto, R., Kose, R., Kono, H., Matsushima, T., Isono, T., Yamamoto, T. & Satoh, T. 2017. One-step production of amphiphilic nanofibrillated cellulose using a cellulose-producing bacterium. Biomacromolecules, 18: 3432-3438.

Tunna, T.S., Zaidul, I.S.M., Ahmed, Q.U., Ghafoor K., Al-Juhaimi, F.Y., Uddin, M.S., Hasan, M. & Ferdous, S. 2015. Analyses and profiling of extract and fractions of neglected weed Mimosa Pudica Linn. traditionally used in Southeast Asia to treat diabetes. South African Journal of Botany, 99: 144-152.

Umamaheswari, S., Malkar, O.S. & Naveena, K. 2017. FTIR spectral and microarchitectural analysis of cellulose produced by Lactococcus lactis under agitated condition. Journal of Pure and Applied Microbiology, 11: 1965-71.

Voon, W.W.Y., Rukayadi, Y. & Meor Hussin A.S. 2015. Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits. Letters in Applied Microbiology, 62(5): 428-433.