This is an outdated version published on 31-10-2023. Read the most recent version.

Isolation of Lactic Acid Bacteria (LAB) from Mimosa pudica (Semalu) for Production of Bacterial Cellulose

https://doi.org/10.55230/mabjournal.v52i4.a050

Authors

  • Lim Bei Min Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
  • Junaidi Zakaria Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
  • Siti Hatijah Mortan Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
  • Shahril Mohamad Faculty of Chemical and Process Engineering Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia
  • Mohd Hairul Ab Rahim Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang Al-Sultan Abdullah, Lebuh Persiaran Tun Khalil Yaakob, 26300, Kuantan, Pahang, Malaysia

Keywords:

Bacterial cellulose, lactic acid bacteria, medicinal plant, microfibril, Mimosa pudica

Abstract

Bacterial cellulose (BC) is a potential eco-friendly biopolymer. BC has higher crystallinity and purity compared to plant cellulose. Scientific studies on the production of BC from lactic acid bacteria (LAB) are minimal compared to other common bacteria such as Acetobacter xylinum. LAB was screened and isolated from different tissues of Mimosa pudica (medicinal plant) using MRS broth and agar as the selective medium. LAB isolates were subjected to 16S rRNA gene sequencing of all the bacterial isolates. BC was produced from all LAB isolates by incubating at 30 °C for 14 days in herbal tea medium (Strobilanthes crispus) and HS medium (control) with 130 r.p.m agitation. BC produced by two selected bacterial isolates was characterized using FESEM, FTIR, XRD, and TGA. Molecular analysis of the 16S rRNA gene of all the potential LAB isolates shows 99.86 - 100% identity to 16S rRNA sequences of other Lactobacillus plantarum strains. Two selected L. plantarum strains (LBM001 & LBM004) produce BC in sphere-like particles with a 1.4 to 2.2 µm diameter range of microfiber. FTIR analysis shows that BC produced by LBM001 and LBM004 have four similar cellulose regions identified in cellulose from other sources, which are O-H stretch (3400-330 cm-1), C-H stretch (2970-2800 cm-1), O-H bending (1620cm-1) and C-O-C stretch (1100-1073 cm-1). XRD analysis shows BC produced by the L. plantarum strains consists of two different XRD peaks at the 2θ angle of 21.53° and 21.85° instead of a single peak (22.76°) identified in the BC produced by A. xylinum and plant cellulose. A similar TG and DTG curved pattern was detected in the BC produced by the L. plantarum strains with the BC produced by A. xylinum and plant cellulose. The LAB isolates from M. pudica have potential in BC production based on the multiple characterization studies.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Andritsou, V., de Melo, E.M., Tsouko, E., Ladakis, D., Maragkoudaki, S., Koutinas, A.A. & Matharu, A.S. 2018. Synthesis and characterization of bacterial cellulose from citrus-based sustainable resources. ACS Omega, 3(8): 10365-10373. DOI: https://doi.org/10.1021/acsomega.8b01315

Baharuddin, N.S., Roslan, M., Bawzer, M., Azzeme, A.M., Rahman, Z.A., Khayat, M.E., Rahman, N. & Sobri, Z.M. 2021. Response surface optimization of extraction conditions and in vitro antioxidant and antidiabetic evaluation of an under-valued medicinal weed, Mimosa pudica. Plants, 10(8): 1692. DOI: https://doi.org/10.3390/plants10081692

Barud, H.S., Ribeiro, C.A., Crespi, M.S., Martines, M.A.U., Dexpert-Ghys, J., Marques, R.F.C., Messaddeq, Y. & Ribeiro, S.J.L. 2007. Thermal characterization of bacterial cellulose-phosphate composite membranes. Journal of Thermal Analysis and Calorimetry, 87: 815-818. DOI: https://doi.org/10.1007/s10973-006-8170-5

Chang, C.P., Wang, I.C., Hung, K.J., & Perng, Y.S. 2010. Preparation and characterization of nanocrystalline cellulose by acid hydrolysis of cotton linter. Taiwan Journal for Science, 25(3): 251-264.

Chua, G.K., Tan, F.H.Y., Chew, F.N. & Mohd-Hairul, A.R. 2019. Nutrients content of food wastes from different sources and its pre-treatment. AIP Conference Proceedings, 2124(1): 020031. DOI: https://doi.org/10.1063/1.5117091

Chua, G.K., Tan, F.H.Y., Chew, F.N., Mohd-Hairul, A.R. & Ahmad, M.A.A. 2020. Food waste hydrolysate as fermentation medium: Comparison of pre-treatment methods. Materials Today: Proceedings, 42: 131-137. DOI: https://doi.org/10.1016/j.matpr.2020.10.399

de Assis, C.A., Houtman, C., Phillips, R., Bilek, E.M.T., Rojas, O.J., Pal, L., Peresin, M.S., Jameel, H. & Gonzalez, R. 2017. Conversion economics of forest biomaterials: Risk and financial analysis of CNC Manufacturing. Biofuels, Bioproducts and Biorefining, 11: 682-700. DOI: https://doi.org/10.1002/bbb.1782

de France, K.J., Hoare, T. & Cranston, E.D. 2017. Review of hydrogels and aerogels containing nanocellulose. Chemistry of Materials, 29: 4609-4631. DOI: https://doi.org/10.1021/acs.chemmater.7b00531

de Melo, E.M., Clark, J.H. & Matharu, A.S. 2017. The Hy-MASS concept: hydrothermal microwave assisted selective scissoring of cellulose for in situ production of (meso)porous nanocellulose fibrils and crystals. Green Chemistry, 19: 3408-3417. DOI: https://doi.org/10.1039/C7GC01378G

Fang, T.T., Mortan, S.H., Mei, L.C., Adzahar, N.S. & Mohd-Hairul, A.R. 2019. Isolation and identification of lactic acid bacteria from selected local medicinal plants. Malaysian Journal of Biochemistry and Molecular Biology, 1: 106-109.

Fasoli, M., Dell'Anna, R., Dal Santo, S., Balestrini, R., Sanson, A., Pezzotti, M., Monti, F. & Zenoni, S. 2016. Pectins, hemicelluloses and celluloses show specific dynamics in the internal and external surfaces of grape berry skin during ripening. Plant Cell Physiology, 57(6): 1332-1349. DOI: https://doi.org/10.1093/pcp/pcw080

Gea, S., Reynolds, C.T., Roohpour, N., Wirjosentono, B., Soykeabkaew, N., Bilotti, E., & Peijs, T. 2011. Investigation into the structural, morphological, mechanical and thermal behaviour of bacterial cellulose after a two-step purification process. Bioresource Technology, 102(19): 9105-9110. DOI: https://doi.org/10.1016/j.biortech.2011.04.077

Gong, J., Li, J., Xu, J., Xiang, Z. & Mo, L. 2017. Research on cellulose nanocrystals produced from cellulose sources with various polymorphs. RSC Advances, 7: 33486-33493. DOI: https://doi.org/10.1039/C7RA06222B

Hashim, N.A.R.N.A., Zakaria, J., Mohamad, S., Mohamad, S.F.S. & Rahim, M.H.A. 2021. Effect of different treatment methods on the purification of bacterial cellulose produced from OPF juice by Acetobacter xylinum. IOP Conference Series: Materials Science and Engineering, 1092(1): 012058. DOI: https://doi.org/10.1088/1757-899X/1092/1/012058

Hospodarova, V., Singovszka, E. & Stevulova, N. 2018. Characterization of cellulosic fibers by FTIR spectroscopy for their further implementation to building materials. American Journal of Analytical Chemistry, 9: 303-310. DOI: https://doi.org/10.4236/ajac.2018.96023

Ismail, M.F., Sabri, N.A., Tajuddin, S.N., Mei, L.C., Mortan, S.H. & Mohd-Hairul, A.R. 2019. Isolation and identification of denitrifying bacteria from indigenous microorganisms. Malaysian Journal of Biochemistry and Molecular Biology, 1: 17-21.

Jagetia, G.C. & Vanlalhruaii, F. 2020. Anticancer potential of Mimosa pudica Linn. Lajwanti in cultured Dalton's ascites lymphoma cells. International Journal of Complementary and Alternative Medicine, 13(3): 91-94. DOI: https://doi.org/10.15406/ijcam.2020.13.00499

Javier-Astete, R., Jimenez-Davalos, J. & Zolla, G. 2020. Determination of hemicellulose, cellulose, holocellulose and lignin content using FTIR in Calycophyllum spruceanum (Benth.) K. Schum. and Guazuma crinita Lam. PLoS ONE, 16(10): e0256559. DOI: https://doi.org/10.1371/journal.pone.0256559

Khan, H., Kadam, A. & Dutt, D. 2020. Studies on bacterial cellulose produced by a novel strain of Lactobacillus genus. Carbohydrate Polymers, 229: 115513. DOI: https://doi.org/10.1016/j.carbpol.2019.115513

Klemm, D., Heublein, B., Fink, H.P. & Bohn, A. 2005. Cellulose: Fascinating biopolymer and sustainable raw material. Angewandte Chemie International Edition, 44: 3358-3393. DOI: https://doi.org/10.1002/anie.200460587

Kumar, A. & Kumar, D. 2014. Isolation and characterization of bacteria from dairy samples of Solan in Himachal Pradesh for identification of Lactobacillus spp. International Journal of Pharmaceutical Sciences Review and Research, 25(110): e114.

Lan, P.T., Huyen, N.T.N., Kim, S,Y., Hang, P.T.N. & Tung, B.T. 2021. Phytochemical analysis and protective effect of ethanolic extract of Mimosa pudica Linn. on methylglyoxal-induced glucotoxicity. Journal of Applied Pharmaceutical Science, 11(9): 93-101.

Manfredi, L.B., Rodríguez, E.S., Wladyka-Przybylak, M., & Vázquez, A. 2006. Thermal degradation and fire resistance of unsaturated polyester, modified acrylic resins and their composites with natural fibres. Polymer degradation and stability, 91(2): 255-261. DOI: https://doi.org/10.1016/j.polymdegradstab.2005.05.003

Sani, A. & Dahman, Y. 2010. Improvements in the production of bacterial synthesized biocellulose nanofibres using different culture methods. Journal of Chemical Technology & Biotechnology, 85(2): 151-164. DOI: https://doi.org/10.1002/jctb.2300

Shi, Z., Zhang, Y., Phillips, G.O. & Yang, G. 2014. Utilization of bacterial cellulose in food. Food Hydrocolloids, 35: 539-545. DOI: https://doi.org/10.1016/j.foodhyd.2013.07.012

Siró, I. & Plackett, D. 2010. Microfibrillated cellulose and new nanocomposite materials: A review. Cellulose, 17: 459-494. DOI: https://doi.org/10.1007/s10570-010-9405-y

Sumardee, N.S.J., Mohd-Hairul, A.R.& Mortan, S.H. 2020. Effect of inoculum size and glucose concentration for bacterial cellulose production by Lactobacillus acidophilus. IOP Conference Series: Materials Science and Engineering, 991(1): 012054. DOI: https://doi.org/10.1088/1757-899X/991/1/012054

Sun, Y., Lin, L., Deng, H., Li, J., He, B., Sun, R. & Ouyang, P. 2008. Structural changes of bamboo cellulose in formic acid. BioResources, 3(2): 297-315. DOI: https://doi.org/10.15376/biores.3.2.297-315

Tajima, K., Kusumoto, R., Kose, R., Kono, H., Matsushima, T., Isono, T., Yamamoto, T. & Satoh, T. 2017. One-step production of amphiphilic nanofibrillated cellulose using a cellulose-producing bacterium. Biomacromolecules, 18: 3432-3438. DOI: https://doi.org/10.1021/acs.biomac.7b01100

Tunna, T.S., Zaidul, I.S.M., Ahmed, Q.U., Ghafoor K., Al-Juhaimi, F.Y., Uddin, M.S., Hasan, M. & Ferdous, S. 2015. Analyses and profiling of extract and fractions of neglected weed Mimosa Pudica Linn. traditionally used in Southeast Asia to treat diabetes. South African Journal of Botany, 99: 144-152. DOI: https://doi.org/10.1016/j.sajb.2015.02.016

Umamaheswari, S., Malkar, O.S. & Naveena, K. 2017. FTIR spectral and microarchitectural analysis of cellulose produced by Lactococcus lactis under agitated condition. Journal of Pure and Applied Microbiology, 11: 1965-71. DOI: https://doi.org/10.22207/JPAM.11.4.38

Voon, W.W.Y., Rukayadi, Y. & Meor Hussin A.S. 2015. Isolation and identification of biocellulose-producing bacterial strains from Malaysian acidic fruits. Letters in Applied Microbiology, 62(5): 428-433. DOI: https://doi.org/10.1111/lam.12568

Published

31-10-2023

Versions

How to Cite

Min, L. B., Zakaria, J., Mortan, S. H., Mohamad, S. ., & Ab Rahim, M. H. (2023). Isolation of Lactic Acid Bacteria (LAB) from Mimosa pudica (Semalu) for Production of Bacterial Cellulose. Malaysian Applied Biology, 52(4), 85–95. https://doi.org/10.55230/mabjournal.v52i4.a050

Issue

Vol. 52 No. 4 (2023): Special Issue: International Conference on Industry-Academia Initiatives in Biotechnology and Chemistry 2021 (iCIABC2021)

Section

Research Articles
Copyright & Licensing
License

Any reproduction of figures, tables and illustrations must obtain written permission from the Chief Editor (wicki@ukm.edu.my). No part of the journal may be reproduced without the editor’s permission

Crossref
Scopus
Google Scholar
Europe PMC

Funding data