Cytotoxic Effect of Bajong LN Rice Methanol Extract on Human Squamous Cell Carcinoma, ORL-48
Keywords:
Bajong LN rice, cisplatin, cytotoxicity, MTS assay, ORL-48 cellsAbstract
Oral squamous cell carcinoma (OSCC), a major oral cancer, significantly challenges treatments and impacts patient quality of life; current therapies often cause severe side effects, highlighting the urgent need for gentler alternatives. Rice stands as one of the primary cereal grains providing the daily caloric intake for more than half of the global population. Extensive research has demonstrated the significant health benefits derived from rice, attributed to its abundance of bioactive compounds. This study endeavours to explore the potential cytotoxic effects of Bajong LN rice, a pigmented purple rice indigenous to Sarawak, on human squamous cell carcinoma, ORL-48 cells. Cells were cultured in complete DMEM/F-12 media and incubated under standard culture conditions. Upon reaching 80% confluency, the cells were treated to varied concentrations (ranging from 0 μg/ml to 2000 μg/ml) of Bajong LN rice methanol extract (BLN-ME) and cisplatin. Subsequently, the cells were incubated for 48 and 72 hours, and their cytotoxicity was assessed using the MTS assay. Results demonstrated that cisplatin inhibited ORL-48 cells with an IC50 of 7.483 μg/ml and 3.877 μg/ml; and an IC80 of 40.649 μg/ml; and 17.543 μg/ml for 48 and 72 hours, respectively. Correspondingly, BLN-ME exhibited a notable cytotoxic effect against ORL-48 cells at 48- and 72-hour intervals, with an IC50 of 354.4 μg/ml and 342.0 μg/ml; and an IC80 of 450.3 μg/ml and 423.63 μg/ml, respectively. The cytotoxic activity of BLN-ME against ORL-48 cells was observed in both a time and dose-dependent manner. Morphological analysis and the Trypan blue exclusion assay corroborated the MTS assay's findings. Our preliminary findings provide the first scientific evidence of the cytotoxic effect of BLN-ME specifically against human squamous cell carcinoma, ORL-48 cells. This study suggests the potential of BLN-ME as a promising anti-cancer agent, presenting opportunities for further investigation into its underlying cytotoxic mechanisms.
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Asenye, G.E., Akinleye, T.E., George, U.E., Mgbeje, B.I., Adewumi, O.M., Adeniji, J.A., Ogbole, O.O. & Ebong, P.E. 2023. Cytotoxic action of the leaves of Uvaria chamae P. Beauv. and Dicliptera paniculata (Forssk.) I. Darbysh. from Nigeria mediated through intrinsic apoptotic pathway induction in four cancer cell lines. Phytomedicine Plus, 3(2): 100423. DOI: https://doi.org/10.1016/j.phyplu.2023.100423
Avanthi, P. & Vigasini, K.N. 2021. In vitro antioxidant and anticancer activity of ethanolic extract of defatted rice bran on MCF-7 (breast) and A549 (lung) cancer cell lines. Asian Journal of Biological and Life Sciences, 10: 210-216. DOI: https://doi.org/10.5530/ajbls.2021.10.30
Bahuguna, A., Khan, I., Bajpai, V.K. & Kang, S.C. 2017. MTT assay to evaluate the cytotoxic potential of a drug. Bangladesh Journal of Pharmacology, 12(2): 115-118. DOI: https://doi.org/10.3329/bjp.v12i2.30892
Banjerdpongchai, R., Wudtiwai, B. & Sringarm, K. 2013. Cytotoxic and apoptotic-inducing effects of purple rice extracts and chemotherapeutic drugs on human cancer cell lines. Asian Pacific Journal of Cancer Prevention, 14(11): 6541-6548. DOI: https://doi.org/10.7314/APJCP.2013.14.11.6541
Brown, T., Punyamurtula, U., Strandberg, J. & El-Deiry, W.S. 2022. Cell density-related variability in chemotherapeutic resistance patterns in human cancer cells. Cancer Research, 82(12): 1008-1008. DOI: https://doi.org/10.1158/1538-7445.AM2022-1008
Canga, I., Vita, P., Oliveira, A.I., Castro, M.Á. & Pinho, C., 2022. In vitro cytotoxic activity of African plants: A review. Molecules, 27(15): 4989. DOI: https://doi.org/10.3390/molecules27154989
Chen, C.F., Hwang, J.M., Lee, W., Chiang, H.C., Lin, J.C. & Chen, H.Y. 1988. Search for anti-tumor agents from Chinese herbs. I. Anti-tumor screening method. Zhonghua yi xue za zhi= Chinese Medical Journal; Free China ed, 41(3): 177-184.
Dolfi, S.C., Yang, Z., Lee, M.J., Guan, F., Hong, J. & Yang, C.S. 2013. Inhibitory effects of different forms of tocopherols, tocopherol phosphates, and tocopherol quinones on growth of colon cancer cells. Journal of agricultural and food chemistry, 61(36): 8533-8540. DOI: https://doi.org/10.1021/jf401076g
Falco, A., de Oliveira, T.B., Cacicedo, J., Ospina, A.V., Ticona, M.Á., Galindo, H., Pereira, M.D., Aguilar-Ponce, J.L., Rueda-Domínguez, A., Soria, T. & Taberna, M. 2021. Ibero-American expert consensus on squamous cell carcinoma of the head and neck treatment in patients unable to receive cisplatin: recommendations for clinical practice. Cancer Management and Research, 2021(13): 6689-6703. DOI: https://doi.org/10.2147/CMAR.S322411
Fallahi-Sichani, M., Honarnejad, S., Heiser, L.M., Gray, J.W. & Sorger, P.K. 2013. Metrics other than potency reveal systematic variation in responses to cancer drugs. Nature Chemical Biology, 9(11): 708-714. DOI: https://doi.org/10.1038/nchembio.1337
Geran, R.I., Greenberg, N.H, Mac Donald, M.M. & Schumacher, A. 1972. Protocols for screening chemical agents and natural products against animal tumors and other biological systems. Cancer Chemother Rept, 103: 1-13.
Ghasemi, M., Turnbull, T., Sebastian, S. & Kempson, I. 2021. The MTT assay: Utility, limitations, pitfalls, and interpretation in bulk and single-cell analysis. International Journal of Molecular Sciences, 22(23): 2827. DOI: https://doi.org/10.3390/ijms222312827
González-Ruiz, I., Ramos-García, P., Ruiz-Ávila, I. & González-Moles, M.Á. 2023. Early diagnosis of oral cancer: A complex polyhedral problem with a difficult solution. Cancers, 15(13): 3270. DOI: https://doi.org/10.3390/cancers15133270
Hartati, F.K. 2022. Black rice extract induced apoptosis and cell cycle arrest on human cancer cell lines. Current Research in Nutrition & Food Science, 10(3): 971-979. DOI: https://doi.org/10.12944/CRNFSJ.10.3.14
Hui, C., Bin, Y., Xiaoping, Y., Long, Y., Chunye, C., Mantian, M. & Wenhua, L. 2010. Anticancer activities of an anthocyanin-rich extract from black rice against breast cancer cells in vitro and in vivo. Nutrition and Cancer, 62(8): 1128-1136. DOI: https://doi.org/10.1080/01635581.2010.494821
Imbesi Bellantoni, M., Picciolo, G., Pirrotta, I., Irrera, N., Vaccaro, M., Vaccaro, F. Squadrito. & Pallio, G. 2023. Oral cavity squamous cell carcinoma: An update of the pharmacological treatment. Biomedicines, 11(4): 1112. DOI: https://doi.org/10.3390/biomedicines11041112
Ishikawa, K., Yoshida, K., Kanie, K., Omori, K. & Kato, R., 2019. Morphology-based analysis of myoblasts for prediction of myotube formation. SLAS DISCOVERY: Advancing Life Sciences R&D, 24(1): 47-56. DOI: https://doi.org/10.1177/2472555218793374
Kasiram, M.Z., Hapidin, H., Abdullah, H., Ahmad, A. & Sulong, S. 2021. Combination therapy of cisplatin and other agents for osteosarcoma: a review. Current Cancer Therapy Reviews, 17(2): 137-147. DOI: https://doi.org/10.2174/1573394716999201016160946
Kumarathilaka, P., Seneweera, S., Meharg, A. & Bundschuh, J. 2018. Arsenic speciation dynamics in paddy rice soil-water environment: sources, physico-chemical, and biological factors-a review. Water research, 140: 403-414. DOI: https://doi.org/10.1016/j.watres.2018.04.034
Molinari, B.L., Tasat, D.R., Palmieri, M.A., O'Connor, S.E. & Cabrini, R.L., 2003. Cell-based quantitative evaluation of the MTT assay. Analytical and Quantitative Cytology and Histology, 25(5): 254-262.
Nascimento, E. de A., Coutinho, L. de L., Silva, C. J. da., Lima, V. L. A. de. & Aguiar, J. dos S. 2022. In vitro anticancer properties of anthocyanins: A systematic review. Biochimica et Biophysica Acta (BBA)-Reviews on Cancer, 1877(4): 188748. DOI: https://doi.org/10.1016/j.bbcan.2022.188748
Pei, J., Panina, S.B. & Kirienko, N.V. 2020. An automated differential nuclear staining assay for accurate determination of mitocan cytotoxicity. JoVE (Journal of Visualized Experiments), (159): e61295. DOI: https://doi.org/10.3791/61295-v
Perrut, M. & Perrut, V. 2018. Towards ingredients by combining Supercritical Fluids with other processes. The Journal of Supercritical Fluids, 134: 214-219. DOI: https://doi.org/10.1016/j.supflu.2017.11.019
Piccinini, F., Tesei, A., Arienti, C. & Bevilacqua, A. 2017. Cell counting and viability assessment of 2D and 3D cell cultures: expected reliability of the trypan blue assay. Biological Procedures Online, 19: 1-12. DOI: https://doi.org/10.1186/s12575-017-0056-3
Ren, Z.W., Kopittke, P.M., Zhao, F.J. & Wang, P. 2023. Nutrient accumulation and transcriptome patterns during grain development in rice. Journal of Experimental Botany, 74(3): 909-930. DOI: https://doi.org/10.1093/jxb/erac426
Rukmana, R.M., Soesilo, N.P. & Rumiyati, P.R. 2016. The effect of ethanolic extract of black and white rice bran (Oryza sativa L.) on cancer cells. Indonesian Journal of Biotechnology, 21(1): 63-69. DOI: https://doi.org/10.22146/ijbiotech.26814
Sancho-Martínez, S.M., Piedrafita, F.J., Cannata-Andía, J.B., López-Novoa, J.M. & López-Hernández, F.J. 2011. Necrotic concentrations of cisplatin activate the apoptotic machinery but inhibit effector caspases and interfere with the execution of apoptosis. Toxicological sciences, 122(1): 73-85. DOI: https://doi.org/10.1093/toxsci/kfr098
Shammugasamy, B., Ramakrishnan, Y., Ghazali, H.M. & Muhammad, K. 2015. Tocopherol and tocotrienol contents of different varieties of rice in Malaysia. Journal of the Science of Food and Agriculture, 95(4): 672-678. DOI: https://doi.org/10.1002/jsfa.6742
Ude, A., Afi-Leslie, K., Okeke, K. & Ogbodo, E. 2022. Trypan blue exclusion assay, neutral red, acridine orange and propidium iodide. In: Cytotoxicity. A. Sukumaran and M.A. Mansour (Eds.). IntechOpen, pp.1-13. DOI: https://doi.org/10.5772/intechopen.105699
Uttama, S. & Ittharat, A. 2010. Cytotoxic activity of Hommali 105 rice bran extract against human cancer cells. Thai Journal of Pharmacology, 32(1): 102-105.
Webb, N.E., Montefiori, D.C. & Lee, B. 2015. Dose-response curve slope helps predict therapeutic potency and breadth of HIV broadly neutralizing antibodies. Nature Communications, 6(1): 8443. DOI: https://doi.org/10.1038/ncomms9443
Weinstein, J.N. & Lorenzi, P.L. 2013. Discrepancies in drug sensitivity. Nature, 504(7480): 381-383. DOI: https://doi.org/10.1038/nature12839
Wen, T.X. 2015. Extraction and Analysis of Antioxidant Capacity in Rice Bran Extracts from Different Sarawak Local Rice Varieties. Swinburne University of Technology.
Yang, C.S., Luo, P., Zeng, Z., Wang, H., Malafa, M. & Suh, N. 2020. Vitamin E and cancer prevention: Studies with different forms of tocopherols and tocotrienols. Molecular Carcinogenesis, 59(4): 365-389. DOI: https://doi.org/10.1002/mc.23160
Zhou, W., Lv, T.F., Chen, Y., Westby, A.P. & Ren, W.J. 2014. Soil physicochemical and biological properties of paddy-upland rotation: A review. The Scientific World Journal, 2014: 1-8. DOI: https://doi.org/10.1155/2014/856352
Zulkapli, R. & Abdul Razak, F. 2019. The preliminary cytotoxicity study: Anti-proliferative activity of aqueous extract of Clinacanthus nutans, Strobilanther crispa and Pereskia bleo on oral squamous carcinoma cells ORL-48. Proceeding Book of 9th Dental Students' Scientific Symposium, Volume 1, pp.20-21.
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