Unveiling the Diversity and Ecological Roles of Macrofungi in Ayer Hitam Forest Reserve, Selangor, Malaysia
Keywords:
Basidiomycetes, Macrofungi, Mycorrhizal, Parasites, Saprophytes, urbanAbstract
The biodiversity of macrofungi in remaining lowland dipterocarp forest in highly urbanization pressures is infrequently studied, even though their significant impact on the ecosystem of forested and non-forested habitats. Therefore, this research endeavours to unravel the diversity of macrofungi from different substrates in the Ayer Hitam Forest Reserve located in Selangor, Malaysia. Through a combination of field surveys, the study seeks to contribute valuable insights into the complex relationships between macrofungi and the surrounding habitats where macrofungal community structure was mainly influenced by substrate richness and microclimates. Sporocarps of macrofungi were collected from selected sites using opportunistic sampling methods. A total of 333 sporocarps were obtained and identified based on morphological and molecular analysis. Basidiomycota prevailed in the Ayer Hitam Forest Reserve where 27 species belonging to 14 families were successfully identified. Saprophytic fungi (22 species, 81.5%) dominated the areas, and a small number of mycorrhizal (1 species, 3.7%) and parasitic (4 species, 14.8%) fungi were found. Based on species diversity, order Polyporales is the highest occurrence species in the sampling areas with a Shannon-Weiner Index value of 2.103 and Simpson Index value of 0.954, Evenness Index value of 0.601, making it the most abundant order containing Microporus species, The findings are expected to enhance our knowledge of the biodiversity of macrofungi from different substrates in Ayer Hitam Forest Reserve, which can lead to conservation efforts and promote the ecological significance of macrofungi in tropical forest ecosystems.
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Alem, D., Dejene, T., Andr, J. & Martín-pinto, P. 2021. Survey of macrofungal diversity and analysis of edaphic factors influencing the fungal community of church forests in dry Afromontane areas of Northern Ethiopia. Forest Ecology and Management, 496: 119-391. DOI: https://doi.org/10.1016/j.foreco.2021.119391
Arenas, F., Navarro-Ródenas, A., Marqués-Gálvez, J.E., Ghignone, S., Mello, A. & Morte, A. 2021. Different patterns in root and soil fungal diversity drive plant productivity of the desert truffle Terfezia claveryi in plantation. Environmental Microbiology, 23(10): 5917-5933. DOI: https://doi.org/10.1111/1462-2920.15688
Bahari, M.N.A., Sakeh, N.M., Abdullah, S.N.A., Ramli, R.R. & Kadkhodaei, S. 2018. Transciptome profiling at early infection of Elaeis guineensis by Ganoderma boninense provides novel insights on fungal transition from biotrophic to necrotrophic phase. BMC Plant Biology, 18(1):1-25. DOI: https://doi.org/10.1186/s12870-018-1594-9
Bolhassan, M.H., Abdullah, N., Sabaratnam, V., Tsutomu, H., Abdullah, S., Mohd, N., Rashid, N. & Musa, Y. 2012. Diversity and distribution of Polyporales in Peninsular Malaysia. Sains Malaysiana, 41(2): 155-161.
Caboň, M., Eberhardt, U., Looney, B., Hampe, F., Kolařík, M., Jančovičová, S., Verbeken, A. & Adamčík, S. 2017. New insights in Russula subsect. Rubrinae: Phylogeny and the quest for synapomorphic characters. Mycological Progress, 16(9): 877-892. DOI: https://doi.org/10.1007/s11557-017-1322-0
Castaño, C., Alday, J.G., Lindahl, B.D., de Aragón, J.M., de-Miguel, S., Colinas, C., Parladé, Pera, J. & Bonet, J.A. 2018. Lack of thinning effects over inter-annual changes in soil fungal community and diversity in a Mediterranean pine forest. Forest Ecology and Management, 424: 420-427. DOI: https://doi.org/10.1016/j.foreco.2018.05.004
Chen, Y., Svenning, J., Wang, X., Cao, R. & Yuan, Z. 2018. Drivers of macrofungi community structure differ between soil and rotten-wood substrates in a temperate mountain forest in China. Frontiers in Microbiology, 9(1): 1-9. DOI: https://doi.org/10.3389/fmicb.2018.00037
Chong, K.P., Dayou, J. & Alexander, A. 2017. Pathogenic nature of Ganoderma boninense and basal stem rot disease. In: Detection and control of Ganoderma boninense in oil palm crop. K.P. Chong, J. Dayou and A. Alexander (Eds.). Springer. pp. 5-12. DOI: https://doi.org/10.1007/978-3-319-54969-9_2
Chua, L.S.L., Lee, S.S., Alias, S.A., Jones, E.G.B., Zainuddin, N. & Chan, H.T. 2012. Checklist of Fungi of Malaysia, Issue/No. 132. Forest Research Institute Malaysia (FRIM), Malaysia. pp. 556.
Couceiro, D.M. & Couceiro, S.R.M. 2022. Wood-inhabiting macrofungi Hymenochaetales and Polyporales (Basidiomycota) in the Amazon Forest: Relationship the abiotic factors and substrate colonization. Anais Da Academia Brasileira De Ciencias, 94(3): e20210554. DOI: https://doi.org/10.1590/0001-3765202220210554
Delavaux, C.S., LaManna, J.A., Myers, J.A., Phillips, R.P., Aguilar, S., Allen, D., Alonso, A., Anderson-Teixeira, K.J., Baker, M.E., Baltzer, J.L., Bissiengou, P., Bonfim, M., Bourg, N.A., Brockelman, W.Y., Burslem, D.F.R.P., Chang, L., Chen, Y., Chiang, J., Chu, C. & Averill, C. 2023. Mycorrhizal feedbacks influence global forest structure and diversity. Communications Biology, 6: 1066. DOI: https://doi.org/10.1038/s42003-023-05410-z
Dulay, R.M., Carandang, J.S., Kalaw, S. & Reyes, R. 2020. Distribution and species listing of wild macrofungi in sitio Canding, Barangay Maasin, San Clemente, Tarlac Province, Philippines. Journal Applied Biology and Biotechnology, 8(05): 7-15.
Gallo, A.L., Troncoso, O. & Greslebin, A.G. 2022. Ability of selected wood-inhabiting fungi to degrade in vitro sapwood and heartwood of Nothofagus pumilio. Lilloa, 59: 173-191. DOI: https://doi.org/10.30550/j.lil/2022.59.S/2022.09.24
Goldmann, K., Schöning, I., Buscot, F. & Wubet, T. 2015. Forest management type influences diversity and community composition of soil fungi across temperate forest ecosystems. Frontiers in Microbiology, 6: 1300-1300. DOI: https://doi.org/10.3389/fmicb.2015.01300
He, M.Q., Zhao, R.L., Liu, D.M., Denchev, T.T., Begerow, D., Yurkov, A., Kemler, M., Millanes, A.M., Wedin, M., McTaggart, A.R. & Hyde, K.D. 2022. Species diversity of Basidiomycota. Fungal Diversity, 114: 281-32. DOI: https://doi.org/10.1007/s13225-021-00497-3
Hyde, K.D., Norphanphoun, C., Chen, J., Dissanayake, A.J., Doilom, M., Hongsanan, S., Jayawardena, R.S., Jeewon, R., Perera, R.H., Thongbai, B., Wanasinghe, D.N., Wisitrassameewong, K., Tibpromma, S. & Stadler, M. 2018. Thailand's amazing diversity: up to 96% of fungi in northern Thailand may be novel. Fungal Diversity, 93(1): 215-239. DOI: https://doi.org/10.1007/s13225-018-0415-7
Ifo, S.A., Moutsamboté, J., Koubouana, F., Yoka, J., Ndzai, S.F., Bouetou-Kadilamio, L.N.O., Mampouya, H., Jourdain, C., Bocko, Y.E., Mantota, A.B., Mbemba, M., Mouanga-Sokath, D., Odende, R., Mondzali, L.R., Wenina, Y.E.M., Ouissika, B.C. & Joel, L.J. 2016. Tree species diversity, richness, and similarity in intact and degraded forest in the tropical rainforest of the Congo Basin: Case of the forest of Likouala in the Republic of Congo. International Journal of Forestry Research, 2016: 1-12. DOI: https://doi.org/10.1155/2016/7593681
Jang, S.K. & Hur, T.C. 2014. Relationship between climatic factors and the distribution of higher fungi in Byeonsanbando National Park, Korea. Mycobiology, 42:27-33. DOI: https://doi.org/10.5941/MYCO.2014.42.1.27
Karun, N., Bhagya, B. & Sridhar, K. 2018. Biodiversity of macrofungi in Yenepoya Campus, Southwest India. Microbial Biosystems, 3(1): 1-11. DOI: https://doi.org/10.21608/mb.2018.12354
Kinge, R.T., Apiseh Apalah, N., Mue Nji, T., Neh Acha, A. & Mathias Mih, A. 2017. Species richness and traditional knowledge of macrofungi (mushrooms) in the Awing Forest Reserve and Communities, Northwest Region, Cameroon. Journal of Mycology, 2017: 1-9. DOI: https://doi.org/10.1155/2017/2809239
Leonard, P.L. 2010. A Guide to Collecting and Preserving Fungal Specimens for the Queensland Herbarium. Queensland Herbarium, Department of Environment and Resource Management, Brisbane. 45 pp.
Lindahl, B.D. & Tunlid, A. 2015. Ectomycorrhizal fungi-potential organic matter decomposers, yet not saprotrophs. New Phytologist, 205(4): 1443-1447. DOI: https://doi.org/10.1111/nph.13201
Lynch, M.D. & Thorn, R.G. 2006. Diversity of basidiomycetes in Michigan agricultural soils. Applied and Environmental Microbiology, 72(11):7050-7056. DOI: https://doi.org/10.1128/AEM.00826-06
Min, B.W., Ahrendt, S., Lipzen, A., Toapanta-Alban, C. Blanchette, R.A., Cullen, D., Hibbett, D.S. & Grigoriev, I.V. 2023. Transcriptomics of temporal- versus substrate-specific wood decay in the brown-rot fungus Fibroporia radiculosa. Journal of Fungi, 9(10): 1029-1029. DOI: https://doi.org/10.3390/jof9101029
Mohammadzadeh, S. & Pirzad, A. 2021. Biochemical responses of mycorrhizal-inoculated Lamiaceae (Lavender, Rosemary and Thyme) plants to drought: a field study. Soil Science and Plant Nutrition, 67(1): 41-49. DOI: https://doi.org/10.1080/00380768.2020.1851144
Möller, E.M., Bahnweg, G., Sandermann, H. & Geiger, H.H. 1992. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Research, 20(22): 6115-6116. DOI: https://doi.org/10.1093/nar/20.22.6115
Nakamura, A., Kitching, R.L., Cao, M., Creedy, T.J., Fayle, T.M., Freiberg, M., Hewitt, C.N., Itioka, T., Koh, L.P., Ma, K., Malhi, Y., Mitchell, A., Novotny, V., Ozanne, C.M.P., Song, L., Wang, H. & Ashton, L.A. 2017. Forests and their canopies: Achievements and horizons in Canopy Science. Trends in Ecology and Evolution, 32(6): 438-451. DOI: https://doi.org/10.1016/j.tree.2017.02.020
Niego, A.G.T., Lambert C., Mortimer, P.E.,Thongklang, N., Rapior S., Große, M., Schrey H., Charria-Girón E., Walker A., Hyde K.D. & Stadler M. 2023. The contribution of fungi to the global economy. Fungal Diversity, 121:95-137. DOI: https://doi.org/10.1007/s13225-023-00520-9
Nur 'Aqilah, M.B., Norhidayah, K., Salleh, S., Thi, B.K., Ahmad Fitri, Z., Haja Maideen, K.M. & Nizam, M. 2020. Diversity of macrofungi in a logged-over forest at Bangi Forest Reserve, Selangor, Peninsular Malaysia. Malayan Nature Journal, 72(1): 1-5.
Parlucha, J.A., Soriano, J.K.R., Yabes, M.D., Pampolina, N.M. & Tadiosa, E.R. 2021. Species and functional diversity of macrofungi from protected areas in mountain forest ecosystems of Southern Luzon, Philippines. Tropical Ecology, 62(3): 359-367. DOI: https://doi.org/10.1007/s42965-021-00152-7
Paterson, R.R.M. 2019. Ganoderma boninense disease of oil palm to significantly reduce production after 2050 Sumatra if projected climate occurs. Microorganisms, 7(1): 24. DOI: https://doi.org/10.3390/microorganisms7010024
Pecoraro, L., Wang, X., Venturella, G., Gao, W., Wen, T. & Gafforov, Y. 2020. Molecular evidence supports simultaneous association of the achlorophyllous orchid Chamaegastrodia inverta with ectomycorrhizal Ceratobasidiaceae and Russulaceae. BMC Microbiology, 20:1-13. DOI: https://doi.org/10.1186/s12866-020-01906-4
Priyamvada, H., Akila, M., Singh, R.K., Ravikrishna, R., Verma, R.S., Philip, L., Marathe, R.R., Sahu, L.K., Sudheer, K.P. & Gunthe, S.S. 2017. Terrestrial macrofungal diversity from the tropical dry evergreen biome of Southern India and its potential role in aerobiology. PLoS ONE, 12(1): e0169333. DOI: https://doi.org/10.1371/journal.pone.0169333
Putra, I.P., Amelya, M.P., Veronica, S. & Kurnianto, M.S. 2020. Fantastic fungi around us: a case study of IPB university campus forest. Jurnal Pena Sains, 7(2): 68-82. DOI: https://doi.org/10.21107/jps.v7i2.6753
Ren, Y., Wang, C. & Li, H. 2021. The effect of different climatic conditions on the decomposition of wood by fungi and the interaction between fungal populations. IOP Conference Series: Earth and Environment, 804(4): 042029. DOI: https://doi.org/10.1088/1755-1315/804/4/042029
Rudawska, M., Leski, T., Stasińska, M., Karliński, L., Wilgan, R. & Kujawska, M. 2022. The contribution of forest reserves and managed forests to the diversity of macrofungi of different trophic groups in European mixed coniferous forest ecosystem. Forest Ecology and Management, 518: 120274. DOI: https://doi.org/10.1016/j.foreco.2022.120274
Samsudin, N.I.P. & Abdullah, N. 2019. Edible mushrooms from Malaysia; a literature review on their nutritional and medicinal properties. International Food Research Journal, 26(1):11-31.
Santamaria, B., Verbeken, A. & Haelewaters, D. 2023. Mycophagy: A Global Review of Interactions between invertebrates and fungi. Journal of Fungi, 9(2): 163. DOI: https://doi.org/10.3390/jof9020163
Senanayake, P.D., Mohotti, K. & Paranagama, P. 2016. Identification and substrate utilisation of fungi associated with low country live wood termite, Glyptotermes dilatatus Bugnion & Popoff and the host plant, Camellia sinensis L.O. Kuntze. Journal of The National Science Foundation of Sri Lanka, 44(2): 175. DOI: https://doi.org/10.4038/jnsfsr.v44i2.7998
Senn-Irlet, B. & Bieri, G. 1999. Sporocarp succession of soil-inhabiting macrofungi in an autochthonous subalpine Norway spruce forest of Switzerland. Forest Ecology Management, 124:169-175. DOI: https://doi.org/10.1016/S0378-1127(99)00064-X
Shokrollahi, N., Ho, C.L., Zainudin, N.A.I.M., Wahab, M.A.W.B.A. & Wong, M.Y. 2021. Identification of non-ribosomal peptide synthetase in Ganoderma boninense Pat. that was expressed during the interaction with oil palm. Scientific Reports, 11(1):1-16. DOI: https://doi.org/10.1038/s41598-021-95549-8
Shuhada, S.N., Salim, S., Nobilly, F., Lechner, A.M. & Azhar, B. 2020. Conversion of peat swamp forest to oil palm cultivation reduces the diversity and abundance of macrofungi. Global Ecology and Conservation, 23(2020): e01122. DOI: https://doi.org/10.1016/j.gecco.2020.e01122
Sridhar, K.R., & Karun, N.C. 2019. Diversity and Ecology of Ectomycorrhizal Fungi in the Western Ghats. In: Microbial Interventions in Agriculture and Environment. D. Singh, V. Gupta and R. Prabha (Eds.). Springer, Singapore. pp. 479-507. DOI: https://doi.org/10.1007/978-981-13-8391-5_18
Stallman, J.K. & Robinson, K. 2022. Importance of seasonal variation in Hawaiian mushroom (Agaricomycetes) basidiomata production for biodiversity discovery and conservation. Frontiers Fungal Biology, 3: 869689. DOI: https://doi.org/10.3389/ffunb.2022.869689
Tapwal, A. 2013. Diversity and frequency of macrofungi associated with wet ever green tropical forest in Assam, India. Biodiversitas Journal of Biological Diversity, 14(2):73-78. DOI: https://doi.org/10.13057/biodiv/d140204
Tedersoo, L., May, T.W. & Smith, M.E. 2010. Ectomycorrhizal lifestyle in fungi: Global diversity, distribution, and evolution of phylogenetic lineages. Mycorrhiza, 20:217-263. DOI: https://doi.org/10.1007/s00572-009-0274-x
Thomaes, A., De Keersmaeker, L., De Schrijver, A., Baeten, L., Vandekerkhove, K., Verstraeten, G. & Verheyen, K., 2013. Can soil acidity and light help to explain tree species effects on forest herb layer performance in post-agricultural forests? Plant Soil, 373:183-199. DOI: https://doi.org/10.1007/s11104-013-1786-x
Tomao, A., Bonet, J.A., Pera, J., Castaño, C. & de-Miguel, S. 2020. How does forest management affect fungal diversity and community composition? Current knowledge and future perspectives for the conservation of forest fungi. Forest Ecology and Management, 457:117678. DOI: https://doi.org/10.1016/j.foreco.2019.117678
White, T.J., Bruns, T., Lee, S. & Taylor, J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A guide to Methods and Applications. M. A. Innis, D.H. Gelfand, J.J. Sninsky, T.J. White (Eds.). Academic Press: San Diego, U. pp. 3151322. DOI: https://doi.org/10.1016/B978-0-12-372180-8.50042-1
Ye, L., Li, H., Mortimer, P.E., Xu, J., Gui, H., Karunarathna, S.C., Kumar, A., Hyde, K.D. & Shi, L. 2019. Substrate preference determines macrofungal biogeography in the greater Mekong Sub-Region. Forests, 10(10):824. DOI: https://doi.org/10.3390/f10100824
Yusran, Y., Erniwati, E., Khumaidi, A., Pitopang, R., Rahmawati, R., Korja, I.N., Labiro, E., Rukmi, R., Muthmainnah, M., Maksum, H. & Setyawati, R. 2022. Diversity of macro fungus species in several types of land use around Lore Lindu National Park, Central Sulawesi, Indonesia. International Journal of Design and Nature and Ecodynamics, 17(3):341-351. DOI: https://doi.org/10.18280/ijdne.170303
Zhu, Z., Liu, X., Hsiang, T., Ji, R.Q. & Liu, S. 2023. Forest type and climate outweigh soil bank in shaping dynamic changes in macrofungal diversity in the ancient tree park of Northeast China. Journal of Fungi, 9(8):856-856. DOI: https://doi.org/10.3390/jof9080856
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Universiti Putra Malaysia
Grant numbers GP-IPS/2022/9729200
