Improving The Sporulation of Eimeria tenella Oocysts  Purified From Chicken Faeces

Nurfazila Latif; Aravind Marcus; Paul Bura Thlama; Suhaili Mustafa; Muhamad Hakim Mohd Ali Hanafiah; Lirong Yu Abit; Sharifah Salmah Syed Hussain; Nor Azlina Abdul Aziz; Shu-San Loo; Kiew Lian  Wan; Sarah A Altwaim; Juriah Kamaludeen

doi:10.55230/mabjournal.v54i1.3060

Authors

Nurfazila Latif Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu 97008, Sarawak, Malaysia
Aravind Marcus Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu 97008, Sarawak, Malaysia
Paul Bura Thlama Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu 97008, Sarawak, Malaysia; Veterinary Teaching Hospital, Faculty of Veterinary Medicine, University of Maiduguri, 600230 Bama Road Maiduguri, Borno Nigeria
Suhaili Mustafa Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu 97008, Sarawak, Malaysia
Muhamad Hakim Mohd Ali Hanafiah Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu 97008, Sarawak, Malaysia
Lirong Yu Abit Faculty of Engineering and Applied Science, Curtin Aquaculture Research Laboratories (CARL), Curtin University Malaysia 250, 98009 Miri, Sarawak, Malaysia
Sharifah Salmah Syed Hussain Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
Nor Azlina Abdul Aziz Department of Veterinary Pathology & Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia
Shu-San Loo School of Biosciences, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Semenyih, Selangor Darul Ehsan, Malaysia
Kiew Lian Wan Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia
Sarah A Altwaim Department of Microbiology and Medical Parasitology, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi Arabia
Juriah Kamaludeen
juriahk@upm.edu.my
Department of Animal Science and Fishery, Faculty of Agricultural and Forestry Sciences, Universiti Putra Malaysia Bintulu Sarawak Campus, Bintulu 97008, Sarawak, Malaysia; Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia

Keywords:

Eimeria tenella, Magneto base method, Oocysts, Sporulation

Abstract

Coccidiosis is a major and recurrent intestinal disease in the chicken industry caused mainly by Eimeria tenella and it is the best-known and most economically significant species in poultry. Oocyst purification is a common and critically important method used for the study of Eimeria species. Various methods have been used for the purification of coccidia oocysts. However, no study has outlined the most effective method for increasing the sporulation of Eimeria tenella oocysts. Thus, the study compared and validated the applications of the three common oocyst sporulation methods, viz: (i) forced aeration without a shaker, (ii) forced aeration in combination with a shaker, and (iii) magneto base without forced aeration. A total of 9 individual 21-day-old broiler chickens were inoculated individually with a single 1 mL dose containing 2000 Eimeria tenella oocysts via oral gavage. Water and food were provided ad libitum throughout the experimental period. Pooled group faecal samples were collected on the 7 day post-infection. The average (range) number of sporulated oocysts counts for the forced aeration without shaking was 2250 (1000-2000), forced aeration in combination with shaking was 2000 (1000-1500), and the magneto base without forced aeration was 5500 (2000-3500). The results from the study indicated that the magneto base without forced aeration was the most effective method for enhancing the sporulation of Eimeria tenella oocysts.

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