Effects of Duckweed and Pigments on the Chemical Composition, Physicochemical Properties, and Sensory Attributes of Plant-Based Patties

https://doi.org/10.55230/mabjournal.v55i2.3653

Authors

  • Weini Wong Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia
  • Nur Raudhatul Syahindah Mohd Radzi Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia
  • Wan Mohd Fadli Wan Mokhtar Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia
  • Abdul Manab Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, East Java, Indonesia
  • Ria Dewi Andriani Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, East Java, Indonesia
  • Nurul Huda Postgraduate School, Universitas Brawijaya, Malang 65145, East Java, Indonesia
  • Ishamri Ismail Department of Food Industry, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut Campus, 22200 Besut, Terengganu, Malaysia; Faculty of Animal Science, Universitas Brawijaya, Malang, 65145, East Java, Indonesia

Keywords:

Beet, Leghaemoglobin, Meat analogue, PCA, Protein alternative, Sensory evaluation

Abstract

The present study evaluated the influence of duckweed incorporation (0%, 1%, and 2%) and two pigment types (beet and soy leghaemoglobin, 2%) on the chemical composition, physicochemical properties, visible appearance, and sensory attributes of plant-based patties (PBPs). Increasing duckweed levels improved protein content and moisture retention while reducing cooking loss. Soy leghaemoglobin enhanced redness (a*), whereas beet pigment intensified yellowness (b*). This was also evident in the visible appearance, with leghaemoglobin formulations appearing noticeably redder. Higher duckweed concentrations decreased hardness but lowered sensory acceptance, whereas 1% duckweed, particularly with beet pigment, improved flavour, colour, and overall liking. These results indicate that strategic duckweed inclusion combined with natural pigments can enhance the nutritional value and physicochemical properties of PBPs, although excessive duckweed may compromise sensory quality. Thus, this work offers a new approach to developing PBPs by replacing common ingredients and pigments, supporting advances in future food product development.

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References

Ahmad, M.I., Farooq, S., Alhamoud, Y., Li, C. & Zhang, H. 2023. Soy Leghemoglobin: A review of its structure, production, safety aspects, and food applications. Trends in Food Science & Technology, 141: 104199. DOI: https://doi.org/10.1016/j.tifs.2023.104199

AOAC. 2002. Official methods of analysis. Association of Official Analytical Chemists. 17th Ed. Arlington, United State. 452 pp.

Appenroth, K.-J., Sree, K.S., Bog, M., Ecker, J., Seeliger, C., Böhm, V., Lorkowski, S., Sommer, K., Vetter, W., Tolzin-Banasch, K., Kirmse, R., Leiterer, M., Dawczynski, C., Liebisch, G. & Jahreis, G. 2018. Nutritional value of the duckweed species of the genus Wolffia (Lemnaceae) as human food. Frontiers in Chemistry, 6: 483. DOI: https://doi.org/10.3389/fchem.2018.00483

Bakhsh, A., Cho, C., Baritugo, K.A., Kim, B., Ullah, Q., Rahman, A. & Park, S. 2023a. Characterization of plant-based meat alternatives blended with anthocyanins, chlorophyll, and various edible natural pigments. International Journal of Food Properties, 26(1): 1546-1565. DOI: https://doi.org/10.1080/10942912.2023.2224533

Bakhsh, A., Lee, S.-J., Lee, E.-Y., Sabikun, N., Hwang, Y.-H. & Joo, S.-T. 2021. A novel approach for tuning the physicochemical, textural, and sensory characteristics of plant-based meat analogs with different levels of methylcellulose concentration. Foods, 10(3): 560. DOI: https://doi.org/10.3390/foods10030560

Bakhsh, A., Park, J., Baritugo, K.A., Kim, B., Sil Moon, S., Rahman, A. & Park, S. 2023b. A holistic approach toward development of plant-based meat alternatives through incorporation of novel microalgae-based ingredients. Frontiers in Nutrition, 10: 1110613. DOI: https://doi.org/10.3389/fnut.2023.1110613

Chaudhary, S. & Singh, N. 2020. Coloring of food by the use of natural color extracted by beetroot (Beta vulgaris), betalain pigment. Sustainability, Agri, Food and Environmental Research, 9(1): 1-6. DOI: https://doi.org/10.7770/safer-V0N0-art2017

Dhamaratana, S., Methacanon, P. & Charoensiddhi, S. 2025. Chemical composition and in vitro digestibility of duckweed (Wolffia globosa) and its polysaccharide and protein fractions. Food Chemistry Advances, 6: 100867. DOI: https://doi.org/10.1016/j.focha.2024.100867

Furuhashi, M., Morimoto, Y., Shima, A., Nakamura, F., Ishikawa, H. & Takeuchi, S. 2021. Formation of contractile 3D bovine muscle tissue for construction of millimetre-thick cultured steak. npj Science of Food, 5(1): 6. DOI: https://doi.org/10.1038/s41538-021-00090-7

Guo, L., Fang, Y., Jin, Y., He, K. & Zhao, H. 2023. High starch duckweed biomass production and its highly-efficient conversion to bioethanol. Environmental Technology & Innovation, 32: 103296. DOI: https://doi.org/10.1016/j.eti.2023.103296

Ismail, I. & Huda, N. 2022. Chapter 20 - Meat alternatives. In: Future Foods. R. Bhat (Ed.). Academic Press, London. pp. 351-373. DOI: https://doi.org/10.1016/B978-0-323-91001-9.00004-9

Ismail, I., Hwang, Y.-H. & Joo, S.-T. 2019. Interventions of two-stage thermal sous-vide cooking on the toughness of beef semitendinosus. Meat Science, 157: 107882. DOI: https://doi.org/10.1016/j.meatsci.2019.107882

Ismail, I., Hwang, Y.-H. & Joo, S.-T. 2020. Meat analog as future food: A review. Journal of Animal Science and Technology, 62(2): 111-120. DOI: https://doi.org/10.5187/jast.2020.62.2.111

Kumari, S., Alam, A.N., Hossain, M.J., Lee, E.-Y., Hwang, Y.-H. & Joo, S.-T. 2024. Sensory evaluation of plant-based meat: Bridging the gap with animal meat, challenges and future prospects. Foods, 13(1): 108. DOI: https://doi.org/10.3390/foods13010108

Kyriakopoulou, K., Keppler, J.K. & van der Goot, A.J. 2021. Functionality of ingredients and additives in plant-based meat analogues. Foods, 10(3): 600. DOI: https://doi.org/10.3390/foods10030600

Miguel, M.G. 2018. Betalains in some species of the amaranthaceae family: A review. Antioxidants, 7(4): 53. DOI: https://doi.org/10.3390/antiox7040053

Mohd Radzi, N.R.S., Zulkifli, N.A., Huda, N. & Ismail, I. 2025. Assessing the impact of transglutaminase and methylcellulose on physical properties of seitan-infused plant-based meat analog patties compared to beef patties. Indonesian Food Science and Technology Journal, 8(2): 149-157. DOI: https://doi.org/10.22437/ifstj.v8i2.37001

Monego, M.A., Kipper, D.K., de Pellegrini, L.G., Roman, S.S., Kubota, E.H., Prestes, R.C. & de Oliveira Mello, R. 2018. Physicochemical, technological and sensory properties of hamburger made with meat from lambs fed on whole cottonseed. Journal of Food Science and Technology, 55(6): 2230-2239. DOI: https://doi.org/10.1007/s13197-018-3140-z

Nora'zizi, N.F.F., Radzi, N.R.S.M., Zulkifli, N.A., Huda, N., Ismail-Fitry, M.R. & Ismail, I. 2025. Physicochemical, microstructural, and sensory attributes of frozen Buffalo loins treated with sodium tripolyphosphate and sous-vide cooking. Meat Science, 226: 109844. DOI: https://doi.org/10.1016/j.meatsci.2025.109844

Ofoedu, C.E., Bozkurt, H. & Mortimer, J.C. 2025. Towards sustainable food security: Exploring the potential of duckweed (Lemnaceae) in diversifying food systems. Trends in Food Science & Technology, 161: 105073. DOI: https://doi.org/10.1016/j.tifs.2025.105073

On-Nom, N., Promdang, P., Inthachat, W., Kanoongon, P., Sahasakul, Y., Chupeerach, C., Suttisansanee, U. & Temviriyanukul, P. 2023. Wolffia globosa-based nutritious snack formulation with high protein and dietary fiber contents. Foods, 12(14): 2647. DOI: https://doi.org/10.3390/foods12142647

Samard, S. & Ryu, G.-H. 2019. A comparison of physicochemical characteristics, texture, and structure of meat analogue and meats. Journal of the Science of Food and Agriculture, 99(6): 2708-2715. DOI: https://doi.org/10.1002/jsfa.9438

Smith, K.E., Schäfer, M., Lim, M., Robles-Zazueta, C.A., Cowan, L., Fisk, I.D., Xu, S. & Murchie, E.H. 2024. Aroma and metabolite profiling in duckweeds: Exploring species and ecotypic variation to enable wider adoption as a food crop. Journal of Agriculture and Food Research, 18: 101263. DOI: https://doi.org/10.1016/j.jafr.2024.101263

Song, Y., Hu, Z., Liu, S., Luo, S., He, R., Yang, X., Li, S., Yang, X., An, Y. & Lu, Y. 2025. Utilization of Microalgae and Duckweed as Sustainable Protein Sources for Food and Feed: Nutritional Potential and Functional Applications. Journal of Agricultural and Food Chemistry, 73(8): 4466-4482. DOI: https://doi.org/10.1021/acs.jafc.4c11610

Stewart, J.J., Adams, W.W., Escobar, C.M., López-Pozo, M. & Demmig-Adams, B. 2020. Growth and essential carotenoid micronutrients in Lemna gibba as a function of growth light intensity. Frontiers in Plant Science, 11: 480. DOI: https://doi.org/10.3389/fpls.2020.00480

Takács, K., Végh, R., Mednyánszky, Z., Haddad, J., Allaf, K., Du, M., Chen, K., Kan, J., Cai, T., Molnár, P., Bársony, P., Maczó, A., Zalán, Z. & Dalmadi, I. 2025. New insights into duckweed as an alternative source of food and feed: Key components and potential technological solutions to increase their digestibility and bioaccessibility. Applied Sciences, 15(2): 884. DOI: https://doi.org/10.3390/app15020884

Vu, G., Zhou, H. & McClements, D.J. 2022. Impact of cooking method on properties of beef and plant-based burgers: Appearance, texture, thermal properties, and shrinkage. Journal of Agriculture and Food Research, 9: 100355. DOI: https://doi.org/10.1016/j.jafr.2022.100355

Wu, H., Sakai, K., Zhang, J. & McClements, D.J. 2024. Plant-based meat analogs: Color challenges and coloring agents. Food, Nutrition and Health, 1(1): 4. DOI: https://doi.org/10.1007/s44403-024-00005-w

Xia, S., Song, J., Ma, C., Hao, T., Hou, Y., Shen, S., Li, K., Ma, L., Xue, Y., Xue, C. & Jiang, X. 2023. Effects of moisture content and processing temperature on the strength and orientation regulation of fibrous structures in meat analogues. Food Hydrocolloids, 145: 109113. DOI: https://doi.org/10.1016/j.foodhyd.2023.109113

Xu, J., Shen, Y., Zheng, Y., Smith, G., Sun, X.S., Wang, D., Zhao, Y., Zhang, W. & Li, Y. 2023. Duckweed (Lemnaceae) for potentially nutritious human food: A review. Food Reviews International, 39(7): 3620-3634. DOI: https://doi.org/10.1080/87559129.2021.2012800

Zeece, M. 2020. Food colorants. In: Introduction to the Chemistry of Food. M. Zeece (Ed.). Academic Press, London. pp. 313-344. DOI: https://doi.org/10.1016/B978-0-12-809434-1.00008-6

Published

30-06-2026

How to Cite

Wong, W., Mohd Radzi , N. R. S., Wan Mokhtar, W. M. F., Manab, A., Andriani, R. D., Huda, N., & Ismail, I. (2026). Effects of Duckweed and Pigments on the Chemical Composition, Physicochemical Properties, and Sensory Attributes of Plant-Based Patties. Malaysian Applied Biology, 55(2), 64–71. https://doi.org/10.55230/mabjournal.v55i2.3653

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Research Articles

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