CYTOTOXIC EFFECT OF DILLAPIOLE ON HUMAN BREAST CANCER MCF-7 CELLS

INDAH  MOHD AMIN; NUR BATRISYIA RUSLAN; ZULINDA AYU  ZULKIPLI; NURUL AILI  ZAKARIA; MOHD TAUFIQ MAT JALIL; FARIZAN ARIS

doi:10.55230/mabjournal.v51i4.08

Authors

INDAH MOHD AMIN Department of Preclinical Sciences, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buloh 47000, Selangor, Malaysia.
NUR BATRISYIA RUSLAN Department of Preclinical Sciences, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buloh 47000, Selangor, Malaysia.
ZULINDA AYU ZULKIPLI Faculty of Education, Universiti Teknologi MARA, Puncak Alam 42300, Selangor, Malaysia,
NURUL AILI ZAKARIA School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam 40450, Selangor, Malaysia.
MOHD TAUFIQ MAT JALIL School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam 40450, Selangor, Malaysia.
FARIZAN ARIS
farizan@uitm.edu.my
School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, Shah Alam 40450, Selangor, Malaysia. https://orcid.org/0000-0002-7973-4845

Keywords:

anti-cancer, cytotoxic, Dillapiole, MCF-7 cells, WST-1 assay

Abstract

Plant secondary metabolites and their derivatives play a significant role in anticancer drug therapy since they are effective against specific characteristics while reducing side effects. Dillapiole is a phenylpropanoid that holds several bioactivities like anti-fungal, anti-inflammatory, anti-leishmanial, and anti-cancer. This study aims to investigate the possible cytotoxic effect of dillapiole on human breast cancer, MCF-7 cells. Cells were cultured in complete RPMI media and incubated at standard culture conditions. After the cells reached 80% confluency, cells were treated with various concentrations (ranging from 0 µM to 150 µM) of dillapiole and tamoxifen as a positive control. Cells were later incubated at 48 and 72 hours. Using WST-1 assay, the cytotoxic effect was determined for both incubation times. Results show tamoxifen inhibited the MCF-7 cells with the IC50 at 75.9 µM and 39.8 µM for 48 and 72 hours respectively. Parallel with the positive control results, there was a significant cytotoxic effect of dillapiole against MCF-7 cells at 48- and 72-hours incubation with the IC50 at 92.1 µM and 63.1 µM respectively. Based on these results, dillapiole was cytotoxic against MCF-7 cells and its cytotoxic activity was both in a time and dose-dependent manner (<0.05). The morphological analysis supported the WST-1 assay. Our preliminary finding is in agreement with other previous studies, suggesting that dillapiole appears to be a promising anti-cancer agent and opens a wider possibility of downstream analysis on its underlying cytotoxicity mechanism.

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