Screening of Native Fungi For Biodegradation of High-Density Polyethylene (HDPE) Plastic in Mangroves Ecosystem

Ghim Hock Ong; Li Ming Liew; Kok Kee Wong; Rui Rui Wong; Jayanthi  Barasarathi; Khye Er Loh; Tawatchai Tanee

doi:10.55230/mabjournal.v53i6.12

Authors

Ghim Hock Ong
ghimhock.ong@newinti.edu.my
Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia
Li Ming Liew Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia
Kok Kee Wong Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia
Rui Rui Wong Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia
Jayanthi Barasarathi Faculty of Health and Life Sciences, INTI International University, Persiaran Perdana BBN, Putra Nilai, 71800 Nilai, Negeri Sembilan, Malaysia
Khye Er Loh Department of Bioscience, Faculty of Applied Sciences, Tunku Abdul Rahman University of Management and Technology, Jalan Genting Kelang, 53300 Setapak, Kuala Lumpur, Malaysia
Tawatchai Tanee Faculty of Environment and Resource Studies, Mahasarakham University, Thailand

Keywords:

Environmental pollution, Plastic degradation, High-density polyethylene, Sustainable land management

Abstract

Accumulation of high-density polyethylene (HDPE) plastic in the environment has become a global issue. A substantial amount of HDPE wastes ends up in the mangroves posing a significant menace to the ecology. Mitigation techniques using mycoremediation to treat the HDPE are gaining ground due to its sustainable approach. This study aims to screen and identify fungi isolated from a mangrove located in Kampung Kuala Lukut, Malaysia, that can degrade HDPE. From this study, eight fungi species were shown able to grow on HDPE as a sole carbon source in a Bushnell-Hass Broth culture. The identity of these isolates was confirmed using morphological observation and molecular identification using ITS1 and ITS4 primers. The ability of these eight fungi species to degrade plastic was assessed based on the percentage of weight loss of HDPE and the increment of fungal biomass. Results from this study show Aspergillus niger has the highest biomass increment, but Leptobacillium sp. shows the highest percentage of weight loss of HDPE. Thus, Leptobacillium sp. has the best potential to be developed as an efficient agent to degrade HDPE in an integrated plastic waste management system.

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