Profiling The Growth Conditions and Persistent Organic Pollutants (POPS) Tolerance of Phenoliferia glacialis USM-PSY62

Ain Nur Afifah  Azman; Shazilah  Kamaruddin; Doris Huai Xia  Quay; Farah Diba Abu Bakar; Noor Haza Fazlin  Hashim; Abdul Munir Abdul Murad; Izwan  Bharudin

doi:10.55230/mabjournal.v53i4.3118

Authors

Ain Nur Afifah Azman Department of Biological Sciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Shazilah Kamaruddin Department of Biological Sciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Doris Huai Xia Quay Department of Applied Physics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Farah Diba Abu Bakar Department of Biological Sciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Noor Haza Fazlin Hashim Water Quality Laboratory, National Water Research Institute Malaysia (NAHRIM), Ministry of Environment and Water, Jalan Putra Permai, Seri Kembangan 43300, Selangor, Malaysia
Abdul Munir Abdul Murad Department of Biological Sciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia
Izwan Bharudin
ibb@ukm.edu.my
Department of Biological Sciences & Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

Keywords:

Antarctica, cold environment, pollutants, psychrotolerant, yeast

Abstract

Antarctica is characterized by extreme cold, isolated, and unique ecosystems. Nevertheless, Antarctica harbors diverse species of microorganisms, particularly in its ice-covered lakes and subglacial environments. These microorganisms have special adaptations to extreme cold and low-nutrient conditions. Some extremophiles, like psychrophiles can thrive in these harsh environments. Phenoliferia glacialis USM-PSY62, previously identified as Rhodotorula sp. USM-PSY62 is a psychrophilic yeast isolated from the ice brine of Antarctica. However, there is very little information on this psychrophilic yeast. This study aims to characterize the P. glacialis USM-PSY62 through the identification of the optimum growth parameters in different media (Yeast Peptone Dextrose, YPD & Yeast Malt, YM), temperature (4°C, 15°C, 20°C) and pH (6, 7, 8, 9) as well as their ability in carbon assimilation and extracellular enzyme production. It has an optimal growth in YPD compared to YM broth media. P. glacialis USM-PSY62 grows optimally at 15°C and pH 7.0. This Antarctic yeast enters the stationary phase on day six of incubation under optimum conditions. It appeared mainly as elongated-shape and oval-shaped with budding formation and was found to produce extracellular enzymes such as protease and amylase in the presence of 2% glucose concentration in YM media. P. glacialis USM-PSY62 also can assimilate various types of carbon sources including raffinose, arabinose, and maltose. Interestingly, the psychrophilic yeast presented growth in media supplemented with Persistent Organic Pollutants (POPs) such as dichlorophenyldichloroethylene (DDE) and polychlorinated biphenyl (PCB). These preliminary findings suggest that P. glacialis USM-PSY62 has tremendous potential for bioremediation application in polluted cold regions, as well as deepening our knowledge of its optimal growth conditions.

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