• YONG JUN LEE School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
  • DAHMENDRA SRISKANDA School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
  • SREERAMANAN SUBRAMANIAM School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia
  • BEE LYNN CHEW School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia


Ficus carica, organic additives, coconut water, banana extract, potato extract


Fig (Ficus carica L.) belongs to the family of Moraceae and its fruits are known to be nutritious to the human diet. Organic additives function as an alternative to replace conventional carbon sources besides containing other nutritional components required for plant growth. This study aims to evaluate the effects of organic additives incorporated in culture media for the regeneration of Ficus carica cv. Japanese BTM6. In vitro shoots were cultured in MS medium supplemented with 1 mg/L of BAP without sucrose and with different concentrations of organic additives (potato, banana and coconut water). MS medium supplemented with 20 g of sucrose and 1 mg/L of BAP (positive control) showed the highest number of shoots (2.45 ± 0.16), shoot height (4.02 ± 0.18 cm) and number of leaves (8.33 ± 0.44). However, the incorporation of coconut water was observed to also support the induction of shoots, shoot height and leaves. Treatment of 200 mL/L of coconut water was found to significantly improve the number of leaves (5.08 ± 0.81 leaves per explant) in comparison to other organic additives. The study concludes that coconut water can be a feasible alternative as carbon source in the regeneration of Ficus carica cv. Japanese BTM6.


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Aktar, S., Nasiruddin K.M. & Hossain K. 2008. Effects of different media and organic additives interaction on in vitro regeneration of Dendrobium Orchid. Journal of Agriculture & Rural Development, 6(1): 69-74. DOI:

Al-Khateeb, A.A. 2008. Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv. Khanezi by different carbon sources. Bioresource Technology, 99(14): 6550-6555. DOI:

Amini K., Amir J.B. & Alaa E.A.B. 2019. Production, application and health effects of banana pulp and peel flour in the food industry. Journal of Food Science and Technology, 56(2): 548-559. DOI:

Arditti, J. 2009. Micropropagation of Orchids. John Wiley & Sons.

Buah, J. N., Kawamitsu, Y., Yonemori, S., Hayashi, M., & Murayama, S. 2000. Effects of various carbon sources and their combinations on in vitro growth and photosynthesis of banana plantlets. Plant Production Science, 3(4): 392-397. DOI:

Gansau, J.A., Halyena I, Siti N.A., Devina D., Hartinie M. & Roslina J. 2016. Effects of organic additives and plant growth regulators on protocorm development of Dendrobium lowii. Transactions on Science and Technology, 3(3): 462-468.

Gnasekaran, P., Xavier R., Uma R.S. & Subramaniam S. 2010. A study on the use of organic additives on the protocorm-like bodies (PLBS) growth of Phalaenopsis violacea Orchid. Journal of Phytology, 2(1): 29-33.

Goh, J.F. 2016. Effects of selected additives for growth efficiency on in vitro grown Vanilla planifolia Andrews (MSc). Universiti Sains Malaysia.

Hartati, S., Retna B.A., Lili A.S., Ongko C., Arniputri R.B., Soliah L.A. & Cahyono O. 2017. Effects of organic additives and Naphthalene acetic acid (NAA): Application on the in vitro growth of black orchid hybrid (Coelogyne pandurata Lindley). Bulgarian Journal of Agricultural Science, 23(6): 951-957.

Ichihashi, S. & Obaidul M.I. 1999. Effects of complex organic additives on callus growth in three orchid genera, Phalaenopsis, Doritaenopsis, and Neofinetia. Journal of the Japanese Society for Horticultural Science, 68(2): 269-274. DOI:

Kaur, S. & Bhutani K.K. 2012. Organic growth supplement stimulants for in vitro multiplication of Cymbidium pendulum (Roxb.) Sw. Horticultural Science, 39(1): 47-52. DOI:

Lansky, E.P., Helena M.P., Alison D.P. & Robert A.N. 2008. Ficus spp. (Fig): Ethnobotany and potential as anticancer and anti-inflammatory agents. Journal of Ethnopharmacology, 119(2): 195-213. DOI:

Leghari, S.J., Niaz W., Ghulam L., Abdul L., Ghulam B., Khalid H.T., Tofique A., Safdar W. & Ayaz L. 2016. Role of nitrogen for plant growth and development: A review. Advances in Environmental Biology, 10(9): 209-218.

Manawadu, I, Nilanthi D., Senanayakage G. & Nonis S. 2014. Effects of different natural organic additives on in vitro shoot regeneration of Raphanus sativus L. var. Beeralu. Journal of Agricultural Science and Technology A, 4(2014): 219-223.

Mawa, S., Khairana H. & Ibrahim J. 2013. Ficus carica L. (Moraceae): Phytochemistry, traditional uses and biological activities. Evidence-Based Complementary and Alternative Medicine, 2013(3): 974256. DOI:

Molnár, Z., Emese V. & Vince Ö. 2011. Natural substances in tissue culture media of higher plants. Acta Biologica Szegediensis, 55(1): 123-127.

Mondal, S., Mukesh K.A., Mukesh K.S. & Singh R.P. 2015. Effect of coconut water and ascorbic acid on shoot regeneration in banana variety dwarf Cavendish. International Journal of Bioresource, Environment and Agricultural Sciences, 1(1): 65-69.

Murdad, R., Mariam L., Zaleha A. & Rimi R. 2010. Effects of carbon source and potato homogenate on in vitro growth and development of Sabah's endangered orchid: Phalaenopsis gigantea. Asia-Pacific Journal of Molecular Biology and Biotechnology, 18(1): 197-200.

Naik, M., CK, S. & Rawson, A. 2020. Tender coconut water: A review on recent advances in processing and preservation. Food Reviews International, 1-22. DOI:

Nambiar, N., Tee C.S. & Maziah M. 2012. Effects of organic additives and different carbohydrate sources on proliferation of protocorm-like bodies in Dendrobium Alya Pink. Plant Omics Journal, 5(1): 10-18.

Obsuwan, K. & Chockpisit T. 2014. An effect of organic supplements on stimulating growth of Vanda and Mokara seedlings in tissue culture. International Journal of Bioengineering and Life Sciences, 8(7): 696-698.

Prades, A., Dornier, M., Diop, N. & Pain, J.P. 2012. Coconut water uses, composition and properties: A review. Fruits, 67(2): 87-107. DOI:

Smeekens, S., Ma, J., Hanson, J. & Rolland, F. 2010. Sugar signals and molecular networks controlling plant growth. Current Opinion in Plant Biology, 13(3): 273-278. DOI:

Souza, R., Aparecida V., Francyane T.B., Tesfahun A.S., João V.N., Patrícia H.A., Virgínia H.A. & Geraldo M.A.C. 2013. Effect of coconut water on growth of olive embryos cultured in vitro. Ciência Rural, 43(2): 290-296. DOI:

Sriskanda, D., Liew, Y.X., Khor, S.P., Merican, F., Subramaniam, S. & Chew, B.L. 2021. An efficient micropropagation protocol for Ficus carica cv. Golden Orphan suitable for mass propagation. Biocatalysis and Agricultural Biotechnology, 38: 102225. DOI:

Veberic, R., Mateja C. & Franci S. 2008. Phenolic acids and flavonoids of fig fruit (Ficus carica L.) in the Northern Mediterranean region. Food Chemistry, 106(1): 153-157. DOI:

Yee, W. 2015. Feasibility of various carbon sources and plant materials in enhancing the growth and biomass productivity of the freshwater microalgae Monoraphidium griffithii NS16. Bioresource Technology, 196: 1-8. DOI:

Yong, J.W.H., Ge, L., Ng, Y.F. & Tan, S.N. 2009. The chemical composition and biological properties of coconut (Cocos nucifera L.) water. Molecules, 14(12): 5144-5164. DOI:



How to Cite

LEE, Y. J., SRISKANDA, D., SUBRAMANIAM, S., & CHEW, B. L. (2022). THE EFFECTS OF BANANA, POTATO, AND COCONUT WATER IN THE REGENERATION OF Ficus carica cv. Japanese BTM 6. Malaysian Applied Biology, 51(1), 163–170.



Research Articles