Goniothalamin Berkesan Secara In Vitro Terhadap Virus Herpes Manusia Jenis 1 Rintang-Acyclovir

Ainulkhir  Hussin; Norefrina Shafinaz  Md Nor; Adibah Ahamad  Bahtiar; Bohari Mohd  Yamin; Nazlina Ibrahim

doi:10.55230/mabjournal.v51i5.2369

Authors

Ainulkhir Hussin Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia; Pathology Department, Queen Elizabeth Hospital, 88596 Kota Kinabalu, Sabah, Malaysia
Norefrina Shafinaz Md Nor Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia https://orcid.org/0000-0001-7681-4949
Adibah Ahamad Bahtiar Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia
Bohari Mohd Yamin Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia
Nazlina Ibrahim
nazlina@ukm.edu.my
Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor, Malaysia

Keywords:

Aktiviti antivirus, goniothalamin, HHV-1 rintang acyclovir, sebatian terbitan stirilpiron

Abstract

Permasalahan besar yang dihadapi dalam rawatan jangkitan virus herpes manusia jenis I (Human Herpes virus type 1, HHV-1) adalah kehadiran virus mutan yang rintang ubat antivirus iaitu acyclovir. Pencarian ubatan dengan kebolehan mengatasi HHV-1 rintang-acyclovir diperlukan bagi mengatasi masalah ini. Maka, kajian ini bertujuan menentukan konfigurasi Goniothalamin (GTN) yang dipencilkan daripada Goniothalamus umbrosus seterusnya mengenalpasti kebolehannya secara in vitro merencat pencilan HHV-1 mutan yang rintang-acyclovir. Kristal yang terhasil selepas pengekstrakan bahagian akar dan batang G. umbrosus disahkan melalui GC-MS, FTIR dan NMR sebagai GTN. Kiraan puncak gandingan J (J coupling peak calculation) menunjukkan bahawa sebatian aktif ini dalam konfigurasi-cis. Sebelum ujian antivirus dilakukan, nilai kepekatan sitotoksik GTN yang membunuh 50% populasi sel Vero (CC50) ditentukan iaitu 8.747 µg/mL. Kepekatan GTN lebih rendah daripada nilai CC50 digunakan untuk penentuan kesan aktiviti antivirus. Sebanyak sebelas pencilan mutan digunakan bagi menentukan kesan aktiviti antivirus GTN dalam asai pasca-rawatan. Nilai kepekatan berkesan menurunkan 50% pembentukan plak (EC50) adalah antara 0.47 µg/mL (2.35 µM) hingga 1.42 µg/mL (6.42 µM). Nilai indeks pemilihan (SI) iaitu nisbah keberkesanan aktiviti antivirus berbanding kesitotoksikan adalah antara 6 hingga 17 yang menunjukkan bahawa GTN mempunyai potensi baik untuk bertindak sebagai agen antivirus. Kesimpulannya, GTN dalam konfigurasi cis berkesan merencat pencilan virus mutan rintang-acyclovir. Potensi tindakan tersebut penting dalam menangani kehadiran virus rintang-acyclovir terutama yang mempunyai mutasi pada timidin kinase atau polimerase DNA.

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Author Biography

Norefrina Shafinaz Md Nor, Department of Biological Science and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Bangi, 43600, Selangor, Malaysia

Jabatan Sains Biologi dan Bioteknologi,

Fakulti Sains dan Teknologi
Universiti Kebangsaan Malaysia
43600 Bangi
Selangor

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